SILENCING OF G-PROTEIN -SUBUNIT IN BARLEY (Hordeum vulgare L.) BY VIGS AND ITS EFFECT ON THE PLANT GROWTH

Muhammad Qasim SHAHID; Cuneyt UCARLI; Elif KARLIK; Semian Karaer UZUNER; Filiz GUREL

doi:https://doi.org/10.17557/tjfc.75064

Makale detayları

SILENCING OF G-PROTEIN -SUBUNIT IN BARLEY (Hordeum vulgare L.) BY VIGS AND ITS EFFECT ON THE PLANT GROWTH

Yazarlar:

Muhammad Qasim SHAHID | Cuneyt UCARLI | Elif KARLIK | Semian Karaer UZUNER | Filiz GUREL

Yayıncı:

Society of Fields Crop Science

Dergi:

Turkish Journal Of Field Crops 2015 Vol.20 Issue 2, pp.259-264

Belge Tipi:

Makale

Yayın Tarihi:

11/2015

Belge Dili:

İngilizce

Document ID (Org No.):

2000636359

Sıklık:

İki Yıllık

pISSN:

1301-1111

DOI:

https://doi.org/10.17557/tjfc.75064

Özet:

 


G-proteins play important roles in a number of cellular and developmental processes in plants. In this study, we have cloned a 317-bp fragment encoding a part of barley G protein -subunit into -genome of barley stripe mosaic virus (BSMV). Barley seedlings were inoculated with this construct denoted as pSL038-1/G at 3-leaf stage, and assayed for their growth, leaf area and membrane ion leakage. Silencing was achieved after 2 weeks of inoculation and confirmed by the decrease in G subunit mRNAs in barley leaves. There was a significant decrease in plant growth in terms of plant height and leaf area following the viral infection. Leaf area in silenced plants were decreased by >10% compared to control plants (wild-type or inoculated by BSMV-PDS constructs). In conclusion, silencing resulted in etiolated phenotypes and growth retardation. Our optimized VIGS protocol in barley could be adopted to evaluate extensive physiological parameters and molecular changes in G silenced plants.

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Makale detayları

SILENCING OF G-PROTEIN -SUBUNIT IN BARLEY (Hordeum vulgare L.) BY VIGS AND ITS EFFECT ON THE PLANT GROWTH

Yazarlar: Muhammad Qasim SHAHID | Cuneyt UCARLI | Elif KARLIK | Semian Karaer UZUNER | Filiz GUREL

Yayıncı: Society of Fields Crop Science

Dergi: Turkish Journal Of Field Crops 2015 Vol.20 Issue 2, pp.259-264

Belge Tipi: Makale

Yayın Tarihi: 11/2015

Belge Dili: İngilizce

Document ID (Org No.): 2000636359

Sıklık: İki Yıllık

pISSN: 1301-1111

DOI: https://doi.org/10.17557/tjfc.75064

Sayı

Yazarlar:

Muhammad Qasim SHAHID | Cuneyt UCARLI | Elif KARLIK | Semian Karaer UZUNER | Filiz GUREL

Yayıncı:

Society of Fields Crop Science

Dergi:

Turkish Journal Of Field Crops 2015 Vol.20 Issue 2, pp.259-264

Belge Tipi:

Makale

Yayın Tarihi:

11/2015

Belge Dili:

İngilizce

Document ID (Org No.):

2000636359

Sıklık:

İki Yıllık

pISSN:

1301-1111

DOI:

https://doi.org/10.17557/tjfc.75064