PROTECTIVE ROLE OF SELENIUM ON THE HEART OF RATS EXPOSED ACRYLAMIDE

Nasıf Fatih Karakuyu; Halil Aşcı; Mehtap Savran; Kanat Gülle

Makale detayları

PROTECTIVE ROLE OF SELENIUM ON THE HEART OF RATS EXPOSED ACRYLAMIDE

Yazarlar:

Nasıf Fatih Karakuyu | Halil Aşcı | Mehtap Savran | Kanat Gülle

Yayıncı:

Süleyman Demirel University

Ana Başlıklar:

Pharmacology and Pharmaceutical Sciences | Clinical Sciences

Dergi:

Medical Journal of Süleyman Demirel University Vol.30 Issue 1, pp.9-17

Belge Tipi:

Makale

Yayın Tarihi:

3/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001285075

pISSN:

1300-7416

Anahtar kelimeler:

Özet:

 Objective Acrylamide ACR is a harmful organic reactive compound widely used in different industrial fields. Sele- nium SEL is a trace element that takes part in some chemical and enzymatic reactions in both animals and human organisms and can cause some disorders in many systems such as the cardiovascular system in case of deficiency. The aim of this study is to investigate whether SEL has a protective effect on cardiac tissue in experimentally induced ACR toxicity in rats. Material and Method A total of 28 rats were randomly and equally divided into four groups: Control, SEL, ACR, SEL  ACR. At the end of the study, cardiac markers such as creatine kinase MB CKMB, lactate dehydrogenase LDH and ischemia-modified albumin IMA from blood samples were measured. Oxidative stress markers such as total oxidant status TOS, total antioxidant status TAS and oxidative stress index OSI in heart tissue were analyzed. In addition, inducible nitric oxide synthase iNOS activities were determined in heart sections using immunohistochemical methods. Results The ACR  SEL group showed a significant decrease in TOS, OSI, CKMB, LDH, IMA levels and significant increase in TAS level compared to the ACR group. In histopathological and immunohistochemical evaluation of the heart sections; Disorganization in cardiac muscle fibers, increased connective tissue between muscle fibers and irregular eosinophilia in muscle fibers were detected in ACR group. A decrease in histopathological findings was observed in the SEL treated groups compared to the ACR group. iNOS immunoreactivities; moderate marking was observed in the ACR group sections, while poor marking was observed in the ACR  SEL group. Conclusion We determined that ACR caused damage to the heart tissue of rats through various metabolic pathways and that these damages were reversed in the groups given the SEL.

 Objective Acrylamide ACR is a harmful organic reactive compound widely used in different industrial fields. Sele- nium SEL is a trace element that takes part in some chemical and enzymatic reactions in both animals and human organisms and can cause some disorders in many systems such as the cardiovascular system in case of deficiency. The aim of this study is to investigate whether SEL has a protective effect on cardiac tissue in experimentally induced ACR toxicity in rats. Material and Method A total of 28 rats were randomly and equally divided into four groups: Control, SEL, ACR, SEL  ACR. At the end of the study, cardiac markers such as creatine kinase MB CKMB, lactate dehydrogenase LDH and ischemia-modified albumin IMA from blood samples were measured. Oxidative stress markers such as total oxidant status TOS, total antioxidant status TAS and oxidative stress index OSI in heart tissue were analyzed. In addition, inducible nitric oxide synthase iNOS activities were determined in heart sections using immunohistochemical methods. Results The ACR  SEL group showed a significant decrease in TOS, OSI, CKMB, LDH, IMA levels and significant increase in TAS level compared to the ACR group. In histopathological and immunohistochemical evaluation of the heart sections; Disorganization in cardiac muscle fibers, increased connective tissue between muscle fibers and irregular eosinophilia in muscle fibers were detected in ACR group. A decrease in histopathological findings was observed in the SEL treated groups compared to the ACR group. iNOS immunoreactivities; moderate marking was observed in the ACR group sections, while poor marking was observed in the ACR  SEL group. Conclusion We determined that ACR caused damage to the heart tissue of rats through various metabolic pathways and that these damages were reversed in the groups given the SEL.

 Objective Acrylamide ACR is a harmful organic reactive compound widely used in different industrial fields. Sele- nium SEL is a trace element that takes part in some chemical and enzymatic reactions in both animals and human organisms and can cause some disorders in many systems such as the cardiovascular system in case of deficiency. The aim of this study is to investigate whether SEL has a protective effect on cardiac tissue in experimentally induced ACR toxicity in rats. Material and Method A total of 28 rats were randomly and equally divided into four groups: Control, SEL, ACR, SEL  ACR. At the end of the study, cardiac markers such as creatine kinase MB CKMB, lactate dehydrogenase LDH and ischemia-modified albumin IMA from blood samples were measured. Oxidative stress markers such as total oxidant status TOS, total antioxidant status TAS and oxidative stress index OSI in heart tissue were analyzed. In addition, inducible nitric oxide synthase iNOS activities were determined in heart sections using immunohistochemical methods. Results The ACR  SEL group showed a significant decrease in TOS, OSI, CKMB, LDH, IMA levels and significant increase in TAS level compared to the ACR group. In histopathological and immunohistochemical evaluation of the heart sections; Disorganization in cardiac muscle fibers, increased connective tissue between muscle fibers and irregular eosinophilia in muscle fibers were detected in ACR group. A decrease in histopathological findings was observed in the SEL treated groups compared to the ACR group. iNOS immunoreactivities; moderate marking was observed in the ACR group sections, while poor marking was observed in the ACR  SEL group. Conclusion We determined that ACR caused damage to the heart tissue of rats through various metabolic pathways and that these damages were reversed in the groups given the SEL.

 Amaç Akrilamid ACR, farklı endüstriyel alanlarda yaygın olarak kullanılan zararlı organik reaktif bir bileşiktir. Selenyum SEL, hem hayvanlarda hem de insan organizmalarında meydana gelen bazı kimyasal ve enzimatik reaksiyonlarda yer alan ve eksikliği durumunda kardiyovasküler sistem gibi birçok sistemde bazı rahatsızlıklara neden olabilen bir eser elementtir. Bu çalışmanın amacı, sıçanlarda deneysel olarak oluşturulan ACR toksisitesinde SELin kalp dokusu üzerinde koruyucu bir etkisinin olup olmadığını araştırmaktır. Gereç ve Yöntem Toplam 28 sıçan rastgele ve eşit olarak dört gruba ayrıldı: Kontrol, SEL, ACR, SEL  ACR. Çalışma sonunda kan örneklerinden kreatin kinaz MB CKMB, laktat dehidrojenaz LDH ve iskemi modifiye albümin IMA gibi kardiyak belirteçler ile kalp dokusundan toplam oksidan durumu TOS, toplam antioksidan durumu TAS ve oksidatif stres indeksi OSI gibi oksidatif stres belirteçlerinin analizleri yapıldı. Ayıca kalp kesitlerinde immünohistokimyasal yöntemler kullanılarak indüklenebilir nitrik oksit sentaz iNOS aktiviteleri belirlendi. Bulgular ACR  SEL grubunda, ACR grubuna göre TOS, OSI, CKMB, LDH, IMA düzeylerinde anlamlı düşüş ve TAS düzeyinde önemli artış gözlemlendi. Kalp dokusu kesitlerinin histopatolojik ve immünhistokimyasal değerlendirmesinde; ACR grubunda kalp kası liflerinde düzensizlik, kas lifleri arasında bağ dokusu artışı ve kas liflerinde düzensiz eozinofili tespit edildi. SEL uygulanan gruplarda ACR grubuna göre histopatolojik bulgularda azalma gözlendi. iNOS immünreaktiviteleri; ACR grubunda orta düzeyde ekspresyon gözlenirken, ACR  SEL grubunda daha zayıf bir ekspresyon gözlendi. Sonuç ACRnin sıçanların kalp dokusunda çeşitli metabolik yollarla hasara yol açtığını ve bu hasarın SEL verilen gruplarda tersine döndürüldüğünü belirledik.

Makale detayları

PROTECTIVE ROLE OF SELENIUM ON THE HEART OF RATS EXPOSED ACRYLAMIDE

Yazarlar:

Nasıf Fatih Karakuyu | Halil Aşcı | Mehtap Savran | Kanat Gülle

Yayıncı:

Süleyman Demirel University

Ana Başlıklar:

Pharmacology and Pharmaceutical Sciences | Clinical Sciences

Dergi:

Medical Journal of Süleyman Demirel University Vol.30 Issue 1, pp.9-17

Belge Tipi:

Makale

Yayın Tarihi:

3/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001285075

pISSN:

1300-7416

Selenium

acrylamide

antioxidant

rat

Selenyum

akrilamid

antioksidan

sıçan

Sayı

Makale detayları

Yazarlar: Nasıf Fatih Karakuyu | Halil Aşcı | Mehtap Savran | Kanat Gülle

Yayıncı: Süleyman Demirel University

Ana Başlıklar: Pharmacology and Pharmaceutical Sciences | Clinical Sciences

Dergi: Medical Journal of Süleyman Demirel University Vol.30 Issue 1, pp.9-17

Belge Tipi: Makale

Yayın Tarihi: 3/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001285075

pISSN: 1300-7416

Sayı

Yazarlar:

Nasıf Fatih Karakuyu | Halil Aşcı | Mehtap Savran | Kanat Gülle

Yayıncı:

Süleyman Demirel University

Ana Başlıklar:

Pharmacology and Pharmaceutical Sciences | Clinical Sciences

Dergi:

Medical Journal of Süleyman Demirel University Vol.30 Issue 1, pp.9-17

Belge Tipi:

Makale

Yayın Tarihi:

3/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001285075

pISSN:

1300-7416