Determination of Cortisol Hormone from Sweat Samples and Interpretation with Microcontroller

Muhammed Ertuğrul Çapan; Ebru Cingöz; Hasan Uğur Öncel; Ercan Arıcan

Makale detayları

Determination of Cortisol Hormone from Sweat Samples and Interpretation with Microcontroller

Yazarlar:

Muhammed Ertuğrul Çapan | Ebru Cingöz | Hasan Uğur Öncel | Ercan Arıcan

Yayıncı:

Bitlis Eren University

Ana Başlıklar:

Instrumental Methods | Circuits and Systems | Digital Electronic Devices | Electronic Sensors

Dergi:

Bitlis Eren Üniversitesi Fen Bilimleri Dergisi Vol.13 Issue 3, pp.646-658

Belge Tipi:

Makale

Yayın Tarihi:

9/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001327649

pISSN:

2147-3129

Anahtar kelimeler:

Özet:

 Cortisol, known as the bodys stress hormone, regulates metabolism, inflammation, and the immune system. It affects various bodily systems such as the nervous, immune, cardiovascular, respiratory, reproductive, and musculoskeletal systems. Normal sweat cortisol levels range from 8 to 142 ngml, with elevated levels indicating stress 200 ngml in women, 500 ngml in men. This study focuses on colorimetric cortisol analysis using a microfluidic layer to collect sweat samples. Samples are mixed with a specific color reagent. Cortisol levels are quantitatively analyzed using Z-Score values and RGB channel density via photographs taken with the ESP 32 module. The aim is to determine cortisol levels, aiding in diagnosing conditions like Cushings syndrome and Addisons Disease, leveraging wearable technology. The developed system integrates a microcontroller into the wearable microfluidic system for cortisol measurements throughout the day. Data is displayed and recorded on a computer for streamlined cortisol monitoring. The blue tetrazolium method used offers stable colors and rapid reactions 10 minutes. The Limit of Detection LOD is 0.3 ugmL, with a detection limit of 1.1 ugmL. Combined with the ESP 32 Cam module, the system detects cortisol concentrations from 0.8  60 ugmL.

 Cortisol, known as the bodys stress hormone, regulates metabolism, inflammation, and the immune system. It affects various bodily systems such as the nervous, immune, cardiovascular, respiratory, reproductive, and musculoskeletal systems. Normal sweat cortisol levels range from 8 to 142 ngml, with elevated levels indicating stress 200 ngml in women, 500 ngml in men. This study focuses on colorimetric cortisol analysis using a microfluidic layer to collect sweat samples. Samples are mixed with a specific color reagent. Cortisol levels are quantitatively analyzed using Z-Score values and RGB channel density via photographs taken with the ESP 32 module. The aim is to determine cortisol levels, aiding in diagnosing conditions like Cushings syndrome and Addisons Disease, leveraging wearable technology. The developed system integrates a microcontroller into the wearable microfluidic system for cortisol measurements throughout the day. Data is displayed and recorded on a computer for streamlined cortisol monitoring. The blue tetrazolium method used offers stable colors and rapid reactions 10 minutes. The Limit of Detection LOD is 0.3 ugmL, with a detection limit of 1.1 ugmL. Combined with the ESP 32 Cam module, the system detects cortisol concentrations from 0.8  60 ugmL.

 Cortisol, known as the bodys stress hormone, regulates metabolism, inflammation, and the immune system. It affects various bodily systems such as the nervous, immune, cardiovascular, respiratory, reproductive, and musculoskeletal systems. Normal sweat cortisol levels range from 8 to 142 ngml, with elevated levels indicating stress 200 ngml in women, 500 ngml in men. This study focuses on colorimetric cortisol analysis using a microfluidic layer to collect sweat samples. Samples are mixed with a specific color reagent. Cortisol levels are quantitatively analyzed using Z-Score values and RGB channel density via photographs taken with the ESP 32 module. The aim is to determine cortisol levels, aiding in diagnosing conditions like Cushings syndrome and Addisons Disease, leveraging wearable technology. The developed system integrates a microcontroller into the wearable microfluidic system for cortisol measurements throughout the day. Data is displayed and recorded on a computer for streamlined cortisol monitoring. The blue tetrazolium method used offers stable colors and rapid reactions 10 minutes. The Limit of Detection LOD is 0.3 ugmL, with a detection limit of 1.1 ugmL. Combined with the ESP 32 Cam module, the system detects cortisol concentrations from 0.8  60 ugmL.

 Cortisol, known as the bodys stress hormone, regulates metabolism, inflammation, and the immune system. It affects various bodily systems such as the nervous, immune, cardiovascular, respiratory, reproductive, and musculoskeletal systems. Normal sweat cortisol levels range from 8 to 142 ngml, with elevated levels indicating stress 200 ngml in women, 500 ngml in men. This study focuses on colorimetric cortisol analysis using a microfluidic layer to collect sweat samples. Samples are mixed with a specific color reagent. Cortisol levels are quantitatively analyzed using Z-Score values and RGB channel density via photographs taken with the ESP 32 module. The aim is to determine cortisol levels, aiding in diagnosing conditions like Cushings syndrome and Addisons Disease, leveraging wearable technology. The developed system integrates a microcontroller into the wearable microfluidic system for cortisol measurements throughout the day. Data is displayed and recorded on a computer for streamlined cortisol monitoring. The blue tetrazolium method used offers stable colors and rapid reactions 10 minutes. The Limit of Detection LOD is 0.3 ugmL, with a detection limit of 1.1 ugmL. Combined with the ESP 32 Cam module, the system detects cortisol concentrations from 0.8  60 ugmL.

Makale detayları

Determination of Cortisol Hormone from Sweat Samples and Interpretation with Microcontroller

Yazarlar:

Muhammed Ertuğrul Çapan | Ebru Cingöz | Hasan Uğur Öncel | Ercan Arıcan

Yayıncı:

Bitlis Eren University

Ana Başlıklar:

Instrumental Methods | Circuits and Systems | Digital Electronic Devices | Electronic Sensors

Dergi:

Bitlis Eren Üniversitesi Fen Bilimleri Dergisi Vol.13 Issue 3, pp.646-658

Belge Tipi:

Makale

Yayın Tarihi:

9/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001327649

pISSN:

2147-3129

Microfluidic

Cortisol Hormone

Microcontrollers

Colorimetirc Analaysis

Sayı

Makale detayları

Determination of Cortisol Hormone from Sweat Samples and Interpretation with Microcontroller

Yazarlar: Muhammed Ertuğrul Çapan | Ebru Cingöz | Hasan Uğur Öncel | Ercan Arıcan

Yayıncı: Bitlis Eren University

Ana Başlıklar: Instrumental Methods | Circuits and Systems | Digital Electronic Devices | Electronic Sensors

Dergi: Bitlis Eren Üniversitesi Fen Bilimleri Dergisi Vol.13 Issue 3, pp.646-658

Belge Tipi: Makale

Yayın Tarihi: 9/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001327649

pISSN: 2147-3129

Microfluidic

Cortisol Hormone

Microcontrollers

Colorimetirc Analaysis

Sayı

Yazarlar:

Muhammed Ertuğrul Çapan | Ebru Cingöz | Hasan Uğur Öncel | Ercan Arıcan

Yayıncı:

Bitlis Eren University

Ana Başlıklar:

Instrumental Methods | Circuits and Systems | Digital Electronic Devices | Electronic Sensors

Dergi:

Bitlis Eren Üniversitesi Fen Bilimleri Dergisi Vol.13 Issue 3, pp.646-658

Belge Tipi:

Makale

Yayın Tarihi:

9/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001327649

pISSN:

2147-3129