Purification of Buchnericin LB Produced by Lactobacillus buchneri LB

Purification Of Buchnericin Lb Produced BY

Makale detayları

Purification of Buchnericin LB Produced by Lactobacillus buchneri LB

Yazarlar:

Purification Of Buchnericin Lb Produced BY

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2001 Vol.25 Issue 1, pp.59-65

Belge Tipi:

Makale

Yayın Tarihi:

12/2000

Belge Dili:

Türkçe

Document ID (Org No.):

2000813899

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Anahtar kelimeler:

Özet:

 

Buchnericin LB produced by Lactobacillus buchneri LB was purified to homogeneity by a  rapid and simple three-step purification procedure including freeze drying, silicic acid  adsorption/desorption and cation-exchange chromatography. After the silicic acid and cation-exchange  chromatography steps, the activity of buchnericin LB was recovered by 85 and 25%, and its purity  increased about 111 and 2,500 fold, respectively. It was determined that the adsorption of  buchnericin LB to silicic acid and cation-exchange chromatography was dependent on the pH of the  suspending environment. The molecular weight of buchnericin LB was determined to be about 4.0 kDa  by tricine SDS-PAGE.

 

Buchnericin LB produced by Lactobacillus buchneri LB was purified to homogeneity by a  rapid and simple three-step purification procedure including freeze drying, silicic acid  adsorption/desorption and cation-exchange chromatography. After the silicic acid and cation-exchange  chromatography steps, the activity of buchnericin LB was recovered by 85 and 25%, and its purity  increased about 111 and 2,500 fold, respectively. It was determined that the adsorption of  buchnericin LB to silicic acid and cation-exchange chromatography was dependent on the pH of the  suspending environment. The molecular weight of buchnericin LB was determined to be about 4.0 kDa  by tricine SDS-PAGE.

Makale detayları

Purification of Buchnericin LB Produced by Lactobacillus buchneri LB

Yazarlar:

Purification Of Buchnericin Lb Produced BY

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2001 Vol.25 Issue 1, pp.59-65

Belge Tipi:

Makale

Yayın Tarihi:

12/2000

Belge Dili:

Türkçe

Document ID (Org No.):

2000813899

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Bacteriocin

buchnericin LB

Lactobacillus bunchneri LB

purification

silicic acid

cation- exchange chromatography.

Sayı

Makale detayları

Purification of Buchnericin LB Produced by Lactobacillus buchneri LB

Yazarlar: Purification Of Buchnericin Lb Produced BY

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2001 Vol.25 Issue 1, pp.59-65

Belge Tipi: Makale

Yayın Tarihi: 12/2000

Belge Dili: Türkçe

Document ID (Org No.): 2000813899

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Bacteriocin

buchnericin LB

Lactobacillus bunchneri LB

purification

silicic acid

cation- exchange chromatography.

Sayı

Yazarlar:

Purification Of Buchnericin Lb Produced BY

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2001 Vol.25 Issue 1, pp.59-65

Belge Tipi:

Makale

Yayın Tarihi:

12/2000

Belge Dili:

Türkçe

Document ID (Org No.):

2000813899

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152