Effects of vitamin A and C and melatonin on 3-nitrotyrosine formation in guinea pig heart under lipopolysaccharide-induced stress

Halil YAMAN; Tuncer ÇAYCI; Melik SEYREK; Emin Özgür AKGÜL; Yasemin Gülcan KURT; İbrahim AYDIN; Hakan YAREN; Erdinç ÇAKIR; Ömer ÖZCAN; Behzat ÇİMEN; Nurten TÜRKÖZKAN; Mehmet Kemal ERBİL

Makale detayları

Effects of vitamin A and C and melatonin on 3-nitrotyrosine formation in guinea pig heart under lipopolysaccharide-induced stress

Yazarlar:

Halil YAMAN | Tuncer ÇAYCI | Melik SEYREK | Emin Özgür AKGÜL | Yasemin Gülcan KURT | İbrahim AYDIN | Hakan YAREN | Erdinç ÇAKIR | Ömer ÖZCAN | Behzat ÇİMEN | Nurten TÜRKÖZKAN | Mehmet Kemal ERBİL

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2010 Vol.40 Issue 5, pp.715-721

Belge Tipi:

Makale

Yayın Tarihi:

4/2010

Belge Dili:

Türkçe

Document ID (Org No.):

2000754945

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Anahtar kelimeler:

Özet:

 

Bacterial lipopolysaccharide (LPS) triggers synthesis of nitric oxide (NO) and superoxide (O2.-). O2.- can react with NO to produce the powerful oxidant peroxynitrite (ONOO-). The presence of 3-nitrotyrosine (3-NT) in tissues is often used as a marker of ONOO- production. Vitamin A, vitamin C, and melatonin are potent antioxidant molecules. We sought to clarify this issue by examining the effects of vitamin A (15,000 IU/kg/day), vitamin C (600 mg/kg/day), and melatonin (25 mg/kg) on 3-NT formation in heart tissue in a guinea pig model of LPS-induced endotoxemia.   Materials and methods: A total of 75 animals were randomly divided into 5 groups (n = 15 animals for each group). 3-NT levels in the heart tissue were determined by high pressure liquid chromatography with a diode array detector.    Results: In the group given LPS, 3-NT levels were significantly increased compared with the control, vitamin A + LPS-treated, vitamin C + LPS-treated, and melatonin + LPS-treated groups. In the vitamin A + LPS-treated group, vitamin C + LPS-treated group, and melatonin + LPS-treated group, 3-NT levels were similar to those of the control group.   Conclusion: Vitamin A, vitamin C, and melatonin pretreatment significantly prevented 3-NT formation. These agents may offer an advantage in that they could improve the hemodynamics as well as reduce the formation of ONOO-.

 

Bacterial lipopolysaccharide (LPS) triggers synthesis of nitric oxide (NO) and superoxide (O2.-). O2.- can react with NO to produce the powerful oxidant peroxynitrite (ONOO-). The presence of 3-nitrotyrosine (3-NT) in tissues is often used as a marker of ONOO- production. Vitamin A, vitamin C, and melatonin are potent antioxidant molecules. We sought to clarify this issue by examining the effects of vitamin A (15,000 IU/kg/day), vitamin C (600 mg/kg/day), and melatonin (25 mg/kg) on 3-NT formation in heart tissue in a guinea pig model of LPS-induced endotoxemia.   Materials and methods: A total of 75 animals were randomly divided into 5 groups (n = 15 animals for each group). 3-NT levels in the heart tissue were determined by high pressure liquid chromatography with a diode array detector.    Results: In the group given LPS, 3-NT levels were significantly increased compared with the control, vitamin A + LPS-treated, vitamin C + LPS-treated, and melatonin + LPS-treated groups. In the vitamin A + LPS-treated group, vitamin C + LPS-treated group, and melatonin + LPS-treated group, 3-NT levels were similar to those of the control group.   Conclusion: Vitamin A, vitamin C, and melatonin pretreatment significantly prevented 3-NT formation. These agents may offer an advantage in that they could improve the hemodynamics as well as reduce the formation of ONOO-.

Makale detayları

Effects of vitamin A and C and melatonin on 3-nitrotyrosine formation in guinea pig heart under lipopolysaccharide-induced stress

Yazarlar:

Halil YAMAN | Tuncer ÇAYCI | Melik SEYREK | Emin Özgür AKGÜL | Yasemin Gülcan KURT | İbrahim AYDIN | Hakan YAREN | Erdinç ÇAKIR | Ömer ÖZCAN | Behzat ÇİMEN | Nurten TÜRKÖZKAN | Mehmet Kemal ERBİL

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2010 Vol.40 Issue 5, pp.715-721

Belge Tipi:

Makale

Yayın Tarihi:

4/2010

Belge Dili:

Türkçe

Document ID (Org No.):

2000754945

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Key words: Lipopolysaccharide

peroxynitrite

3-nitrotyrosine

vitamin A

vitamin C

melatonin

Sayı

Makale detayları

Effects of vitamin A and C and melatonin on 3-nitrotyrosine formation in guinea pig heart under lipopolysaccharide-induced stress

Yazarlar: Halil YAMAN | Tuncer ÇAYCI | Melik SEYREK | Emin Özgür AKGÜL | Yasemin Gülcan KURT | İbrahim AYDIN | Hakan YAREN | Erdinç ÇAKIR | Ömer ÖZCAN | Behzat ÇİMEN | Nurten TÜRKÖZKAN | Mehmet Kemal ERBİL

Yayıncı: TUBITAK

Dergi: Turkish Journal of Medical Sciences 2010 Vol.40 Issue 5, pp.715-721

Belge Tipi: Makale

Yayın Tarihi: 4/2010

Belge Dili: Türkçe

Document ID (Org No.): 2000754945

Sıklık: İki Aylık

eISSN: 1303-6165

pISSN: 1300-0144

Key words: Lipopolysaccharide

peroxynitrite

3-nitrotyrosine

vitamin A

vitamin C

melatonin

Sayı

Yazarlar:

Halil YAMAN | Tuncer ÇAYCI | Melik SEYREK | Emin Özgür AKGÜL | Yasemin Gülcan KURT | İbrahim AYDIN | Hakan YAREN | Erdinç ÇAKIR | Ömer ÖZCAN | Behzat ÇİMEN | Nurten TÜRKÖZKAN | Mehmet Kemal ERBİL

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2010 Vol.40 Issue 5, pp.715-721

Belge Tipi:

Makale

Yayın Tarihi:

4/2010

Belge Dili:

Türkçe

Document ID (Org No.):

2000754945

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144