Histopathological effects of Montivipera xanthina venom on rats

Hüseyin TOPYILDIZ; Sibel HAYRETDAĞ

Makale detayları

Histopathological effects of Montivipera xanthina venom on rats

Yazarlar:

Hüseyin TOPYILDIZ | Sibel HAYRETDAĞ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Zoology 2012 Vol.36 Issue 4, pp.517-525

Belge Tipi:

Makale

Yayın Tarihi:

3/2012

Belge Dili:

Türkçe

Document ID (Org No.):

2000776347

Sıklık:

İki Aylık

eISSN:

1303-6114

pISSN:

1300-0179

Anahtar kelimeler:

Özet:

 

In this study, the histopathological changes caused by Montivipera xanthina venom to rat skin, skeletal muscle, and liver tissue were analysed by light microscope. The venom (approximately 2.85 mg/kg) was injected intramuscularly into the gastrocnemius muscle of the rats. The animals were dissected 1, 3, and 6 h after the injection time, and tissue samples were taken. Sections 5 µm thick were taken from paraffin blocks of tissue samples, and the sections were stained with haematoxylin and eosin in order to facilitate examination by light microscope. Examinations showed no damage to the epidermis layer of skin. However, damage to the dermis layer, including oedema, haemorrhage, local bleeding, rare inflammatory cells, strong cell infiltration with mixed characteristics, inflammation around the veins, and fat necrosis, was detected. The damage observed in muscular tissue was myonecrosis, mixed character cell infiltration, haemorrhage, and the formation of inclusion on myofibrils. The liver tissue revealed sinusoidal congestion and hepatocellular degeneration.

 

In this study, the histopathological changes caused by Montivipera xanthina venom to rat skin, skeletal muscle, and liver tissue were analysed by light microscope. The venom (approximately 2.85 mg/kg) was injected intramuscularly into the gastrocnemius muscle of the rats. The animals were dissected 1, 3, and 6 h after the injection time, and tissue samples were taken. Sections 5 µm thick were taken from paraffin blocks of tissue samples, and the sections were stained with haematoxylin and eosin in order to facilitate examination by light microscope. Examinations showed no damage to the epidermis layer of skin. However, damage to the dermis layer, including oedema, haemorrhage, local bleeding, rare inflammatory cells, strong cell infiltration with mixed characteristics, inflammation around the veins, and fat necrosis, was detected. The damage observed in muscular tissue was myonecrosis, mixed character cell infiltration, haemorrhage, and the formation of inclusion on myofibrils. The liver tissue revealed sinusoidal congestion and hepatocellular degeneration.

Makale detayları

Histopathological effects of Montivipera xanthina venom on rats

Yazarlar:

Hüseyin TOPYILDIZ | Sibel HAYRETDAĞ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Zoology 2012 Vol.36 Issue 4, pp.517-525

Belge Tipi:

Makale

Yayın Tarihi:

3/2012

Belge Dili:

Türkçe

Document ID (Org No.):

2000776347

Sıklık:

İki Aylık

eISSN:

1303-6114

pISSN:

1300-0179

Key words: Montivipera xanthina

histopathology

venom

skin

skeletal muscle

liver

Sayı

Makale detayları

Histopathological effects of Montivipera xanthina venom on rats

Yazarlar: Hüseyin TOPYILDIZ | Sibel HAYRETDAĞ

Yayıncı: TUBITAK

Dergi: Turkish Journal of Zoology 2012 Vol.36 Issue 4, pp.517-525

Belge Tipi: Makale

Yayın Tarihi: 3/2012

Belge Dili: Türkçe

Document ID (Org No.): 2000776347

Sıklık: İki Aylık

eISSN: 1303-6114

pISSN: 1300-0179

Key words: Montivipera xanthina

histopathology

venom

skin

skeletal muscle

liver

Sayı

Yazarlar:

Hüseyin TOPYILDIZ | Sibel HAYRETDAĞ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Zoology 2012 Vol.36 Issue 4, pp.517-525

Belge Tipi:

Makale

Yayın Tarihi:

3/2012

Belge Dili:

Türkçe

Document ID (Org No.):

2000776347

Sıklık:

İki Aylık

eISSN:

1303-6114

pISSN:

1300-0179