DETERMINATION OF THE EFFICACY OF ENTOMOPATHOGENIC FUNGAL CULTURE FILTRATES ON TETRANYCHUS URTICAE KOCH

Asiye Uzun Yiğit; Şerife Evrim Arıcı; Ozan Demirözer

Makale detayları

DETERMINATION OF THE EFFICACY OF ENTOMOPATHOGENIC FUNGAL CULTURE FILTRATES ON TETRANYCHUS URTICAE KOCH

Yazarlar:

Asiye Uzun Yiğit | Şerife Evrim Arıcı | Ozan Demirözer

Yayıncı:

Mevlüt AKÇURA

Ana Başlıklar:

Agricultural | Veterinary and Food Sciences

Dergi:

Turkish Journal of Agricultural and Natural Sciences Vol.10 Issue 2, pp.292-298

Belge Tipi:

Makale

Yayın Tarihi:

4/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001288347

pISSN:

2148-3647

Anahtar kelimeler:

Özet:

 In this study, lethal effects of pure culture filtrates of 5 different isolates of entomopathogenic fungi Beauveria varroe, Metarhizium robertsii, Purpureocillium lilacinum, Fusarium subglutinans 8A ve F. subglutinans 12A on adult females of Tetranychus urticae Koch were determined. 20 new adult individuals were transferred to bean leaves 4 cm prepared according to the leaf disc method in plastic petri dishes 9 cm. Pure culture filtrates of each entomopathogenic fungus isolate were applied by spraying 4 bar on leaf discs containing adult individuals for 10 seconds. Observations were started 24 hours after the application and continued until the 7th day. Experiments were carried out with 5 replications for the application dose of each entomopathogen isolate. It was found that there was no difference between the mortality rates of T. urticae adults 24 and 96 hours after the culture filtrates were applied P 0.05. 5 days after the application, there was no difference between B. varroe, F. subglutinans 8A, F. subglutinans 12A, and P. lilacinum applications, and M. robertsii 363.67 were statistically different from other entomopathogenic fungus isolates 593.31-663.67 P0.05. On the 7th day, the last observation time, it was determined that B. varroe culture filtrate caused the highest mortality 803.53 on T. urticae adults, and mortality rates in F. subglutinans 12A, F. subglutinans 8A, P. lilacinum and M. robertsii applications were 787.17, 766.0, 734.06, and 462.91, respectively. Mortality rates on T. urticae adults in M. robertsii culture filtrate application were different from other entomopathogenic fungus culture filtrate applications P0.05.

 In this study, lethal effects of pure culture filtrates of 5 different isolates of entomopathogenic fungi Beauveria varroe, Metarhizium robertsii, Purpureocillium lilacinum, Fusarium subglutinans 8A ve F. subglutinans 12A on adult females of Tetranychus urticae Koch were determined. 20 new adult individuals were transferred to bean leaves 4 cm prepared according to the leaf disc method in plastic petri dishes 9 cm. Pure culture filtrates of each entomopathogenic fungus isolate were applied by spraying 4 bar on leaf discs containing adult individuals for 10 seconds. Observations were started 24 hours after the application and continued until the 7th day. Experiments were carried out with 5 replications for the application dose of each entomopathogen isolate. It was found that there was no difference between the mortality rates of T. urticae adults 24 and 96 hours after the culture filtrates were applied P 0.05. 5 days after the application, there was no difference between B. varroe, F. subglutinans 8A, F. subglutinans 12A, and P. lilacinum applications, and M. robertsii 363.67 were statistically different from other entomopathogenic fungus isolates 593.31-663.67 P0.05. On the 7th day, the last observation time, it was determined that B. varroe culture filtrate caused the highest mortality 803.53 on T. urticae adults, and mortality rates in F. subglutinans 12A, F. subglutinans 8A, P. lilacinum and M. robertsii applications were 787.17, 766.0, 734.06, and 462.91, respectively. Mortality rates on T. urticae adults in M. robertsii culture filtrate application were different from other entomopathogenic fungus culture filtrate applications P0.05.

 In this study, lethal effects of pure culture filtrates of 5 different isolates of entomopathogenic fungi Beauveria varroe, Metarhizium robertsii, Purpureocillium lilacinum, Fusarium subglutinans 8A ve F. subglutinans 12A on adult females of Tetranychus urticae Koch were determined. 20 new adult individuals were transferred to bean leaves 4 cm prepared according to the leaf disc method in plastic petri dishes 9 cm. Pure culture filtrates of each entomopathogenic fungus isolate were applied by spraying 4 bar on leaf discs containing adult individuals for 10 seconds. Observations were started 24 hours after the application and continued until the 7th day. Experiments were carried out with 5 replications for the application dose of each entomopathogen isolate. It was found that there was no difference between the mortality rates of T. urticae adults 24 and 96 hours after the culture filtrates were applied P 0.05. 5 days after the application, there was no difference between B. varroe, F. subglutinans 8A, F. subglutinans 12A, and P. lilacinum applications, and M. robertsii 363.67 were statistically different from other entomopathogenic fungus isolates 593.31-663.67 P0.05. On the 7th day, the last observation time, it was determined that B. varroe culture filtrate caused the highest mortality 803.53 on T. urticae adults, and mortality rates in F. subglutinans 12A, F. subglutinans 8A, P. lilacinum and M. robertsii applications were 787.17, 766.0, 734.06, and 462.91, respectively. Mortality rates on T. urticae adults in M. robertsii culture filtrate application were different from other entomopathogenic fungus culture filtrate applications P0.05.

 Bu çalışmada, 5 farklı entomopatojen fungus izolatına Beauveria varroe, Metarhizium robertsii, Purpureocillium lilacinum, Fusarium subglutinans 8A ve F. subglutinans 12A ait saf kültür filtratlarının Tetranychus urticae Kochnin ergin dişileri üzerinde lethal etkileri belirlenmiştir. Plastik petrilerde 9 cm yaprak disk yöntemine göre hazırlanmış fasulye yapraklarına 4 cm 20 yeni ergin birey aktarılmıştır. Her bir entomopatojen fungus izolatına ait saf kültür filtratları püskürtme yöntemiyle 4 bar 10 sn süre ile ergin bireyleri içeren yaprak disklerin üzerine uygulanmıştır. Gözlemlere uygulamadan 24 saat sonra başlanarak 7. güne kadar devam edilmiştir. Denemeler her bir entomopatojen izolatına ait uygulama dozu için 5 tekerrürlü olarak yürütülmüştür. Kültür filtratları uygulandıktan 24 ve 96 saat sonra T. urticae erginlerinde meydana gelen ölüm oranları arasında fark olmadığı bulunmuştur P 0.05. Uygulamadan 5 gün sonra ise B. varroe, F. subglutinans 8A, F. subglutinans 12A ve P. lilacinum uygulamaları arasında fark olmadığı ve M. robertsiinin 363.67 diğer entomopatojen fungus izolatlarından 593.31-663.67 istatistiki olarak farklı olduğu saptanmıştır P0.05. Son gözlem zamanı olan 7. günde, B. varroe kültür filtratının T. urticae erginleri üzerinde en yüksek ölüme 803.53 neden olduğu, ayrıca F. subglutinans 12A, F. subglutinans 8A, P. lilacinum ve M. robertsii uygulamalarında belirlenen ölüm yüzdeleri ise sırasıyla 787.17, 766.00, 734.06, 462.91 olarak saptanmıştır. Metarhizium robertsii kültür filtratı uygulamasındaki T. urticae erginleri üzerindeki ölüm oranları, diğer entomopatojen fungus kültür filtratı uygulamalarından farklı bulunmuştur P0.05.

Makale detayları

DETERMINATION OF THE EFFICACY OF ENTOMOPATHOGENIC FUNGAL CULTURE FILTRATES ON TETRANYCHUS URTICAE KOCH

Yazarlar:

Asiye Uzun Yiğit | Şerife Evrim Arıcı | Ozan Demirözer

Yayıncı:

Mevlüt AKÇURA

Ana Başlıklar:

Agricultural | Veterinary and Food Sciences

Dergi:

Turkish Journal of Agricultural and Natural Sciences Vol.10 Issue 2, pp.292-298

Belge Tipi:

Makale

Yayın Tarihi:

4/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001288347

pISSN:

2148-3647

Agricultural pest, Microbial control, Two-spotted spider miteTarımsal zararlı

Mikrobiyal mücadele

İki noktalı kırmızıörümcek

Sayı

Makale detayları

DETERMINATION OF THE EFFICACY OF ENTOMOPATHOGENIC FUNGAL CULTURE FILTRATES ON TETRANYCHUS URTICAE KOCH

Yazarlar: Asiye Uzun Yiğit | Şerife Evrim Arıcı | Ozan Demirözer

Yayıncı: Mevlüt AKÇURA

Ana Başlıklar: Agricultural | Veterinary and Food Sciences

Dergi: Turkish Journal of Agricultural and Natural Sciences Vol.10 Issue 2, pp.292-298

Belge Tipi: Makale

Yayın Tarihi: 4/2023

Belge Dili: Türkçe

Document ID (Org No.): 2001288347

pISSN: 2148-3647

Agricultural pest, Microbial control, Two-spotted spider miteTarımsal zararlı

Mikrobiyal mücadele

İki noktalı kırmızıörümcek

Sayı

Yazarlar:

Asiye Uzun Yiğit | Şerife Evrim Arıcı | Ozan Demirözer

Yayıncı:

Mevlüt AKÇURA

Ana Başlıklar:

Agricultural | Veterinary and Food Sciences

Dergi:

Turkish Journal of Agricultural and Natural Sciences Vol.10 Issue 2, pp.292-298

Belge Tipi:

Makale

Yayın Tarihi:

4/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001288347

pISSN:

2148-3647