Investigation of Ototoxicity of Intratympanic 5-fluorouracil in Rats

Meltem Demirdağ Çevikkan; Asım Aslan; Elgin Türköz Uluer; Damla Demirkaya Tas

Makale detayları

Investigation of Ototoxicity of Intratympanic 5-fluorouracil in Rats

Yazarlar:

Meltem Demirdağ Çevikkan | Asım Aslan | Elgin Türköz Uluer | Damla Demirkaya Tas

Yayıncı:

Istanbul University

Ana Başlıklar:

Otorhinolaryngology

Dergi:

The Turkish Journal of Ear Nose and Throat Vol.32 Issue 4, pp.115-120

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001283894

pISSN:

2602-4837

Anahtar kelimeler:

Özet:

 Objective: Although 5-fluorouracil is beneficial in treating cholesteatoma in people, it is unknown if this drug harms the inner ear. We are planning to contribute to the literature by investigating the effects of the intratympanic administration of 5-fluorouracil solution form to the middle ear on the inner ear in rats. Materials and Methods: The study was conducted on 11 Wistar albino male rats: a positive control group was treated with amikacin, a study group was treated with 5-FU, and a negative control group received no treatment. One week after intratympanic drug administration, the rats were sacrificed, and ototoxicity was histopathologically examined by light microscopy and TUNEL Terminal deoxynucleotidyl transferasemediated dUTP Nick End Labeling method. Results: There was a significant difference between the two groups treated with amikacin and 5-fluorouracil in terms of apoptosis p0.05. The difference in stria vascularis thicknesses was significant between the amikacin group and the 5-fluorouracil group and the negative control group p0.05. Conclusion: The intratympanic administration of 5-fluorouracil to rats did not have any ototoxic effects, according to the results of the histological analysis that looked at apoptosis.

 Objective: Although 5-fluorouracil is beneficial in treating cholesteatoma in people, it is unknown if this drug harms the inner ear. We are planning to contribute to the literature by investigating the effects of the intratympanic administration of 5-fluorouracil solution form to the middle ear on the inner ear in rats. Materials and Methods: The study was conducted on 11 Wistar albino male rats: a positive control group was treated with amikacin, a study group was treated with 5-FU, and a negative control group received no treatment. One week after intratympanic drug administration, the rats were sacrificed, and ototoxicity was histopathologically examined by light microscopy and TUNEL Terminal deoxynucleotidyl transferasemediated dUTP Nick End Labeling method. Results: There was a significant difference between the two groups treated with amikacin and 5-fluorouracil in terms of apoptosis p0.05. The difference in stria vascularis thicknesses was significant between the amikacin group and the 5-fluorouracil group and the negative control group p0.05. Conclusion: The intratympanic administration of 5-fluorouracil to rats did not have any ototoxic effects, according to the results of the histological analysis that looked at apoptosis.

 Objective: Although 5-fluorouracil is beneficial in treating cholesteatoma in people, it is unknown if this drug harms the inner ear. We are planning to contribute to the literature by investigating the effects of the intratympanic administration of 5-fluorouracil solution form to the middle ear on the inner ear in rats. Materials and Methods: The study was conducted on 11 Wistar albino male rats: a positive control group was treated with amikacin, a study group was treated with 5-FU, and a negative control group received no treatment. One week after intratympanic drug administration, the rats were sacrificed, and ototoxicity was histopathologically examined by light microscopy and TUNEL Terminal deoxynucleotidyl transferasemediated dUTP Nick End Labeling method. Results: There was a significant difference between the two groups treated with amikacin and 5-fluorouracil in terms of apoptosis p0.05. The difference in stria vascularis thicknesses was significant between the amikacin group and the 5-fluorouracil group and the negative control group p0.05. Conclusion: The intratympanic administration of 5-fluorouracil to rats did not have any ototoxic effects, according to the results of the histological analysis that looked at apoptosis.

 Objective: Although 5-fluorouracil is beneficial in treating cholesteatoma in people, it is unknown if this drug harms the inner ear. We are planning to contribute to the literature by investigating the effects of the intratympanic administration of 5-fluorouracil solution form to the middle ear on the inner ear in rats. Materials and Methods: The study was conducted on 11 Wistar albino male rats: a positive control group was treated with amikacin, a study group was treated with 5-FU, and a negative control group received no treatment. One week after intratympanic drug administration, the rats were sacrificed, and ototoxicity was histopathologically examined by light microscopy and TUNEL Terminal deoxynucleotidyl transferasemediated dUTP Nick End Labeling method. Results: There was a significant difference between the two groups treated with amikacin and 5-fluorouracil in terms of apoptosis p0.05. The difference in stria vascularis thicknesses was significant between the amikacin group and the 5-fluorouracil group and the negative control group p0.05. Conclusion: The intratympanic administration of 5-fluorouracil to rats did not have any ototoxic effects, according to the results of the histological analysis that looked at apoptosis.

Makale detayları

Investigation of Ototoxicity of Intratympanic 5-fluorouracil in Rats

Yazarlar:

Meltem Demirdağ Çevikkan | Asım Aslan | Elgin Türköz Uluer | Damla Demirkaya Tas

Yayıncı:

Istanbul University

Ana Başlıklar:

Otorhinolaryngology

Dergi:

The Turkish Journal of Ear Nose and Throat Vol.32 Issue 4, pp.115-120

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001283894

pISSN:

2602-4837

5-fluorouracil

ototoxicity

intratympanic injection

animal model

5-fluorouracil

ototoxicity

intratympanic injection

animal model.

5-fluorouracil

ototoxicity

intratympanic injection

animal model

Sayı

Makale detayları

Yazarlar: Meltem Demirdağ Çevikkan | Asım Aslan | Elgin Türköz Uluer | Damla Demirkaya Tas

Yayıncı: Istanbul University

Ana Başlıklar: Otorhinolaryngology

Dergi: The Turkish Journal of Ear Nose and Throat Vol.32 Issue 4, pp.115-120

Belge Tipi: Makale

Yayın Tarihi: 1/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001283894

pISSN: 2602-4837

Sayı

Yazarlar:

Meltem Demirdağ Çevikkan | Asım Aslan | Elgin Türköz Uluer | Damla Demirkaya Tas

Yayıncı:

Istanbul University

Ana Başlıklar:

Otorhinolaryngology

Dergi:

The Turkish Journal of Ear Nose and Throat Vol.32 Issue 4, pp.115-120

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001283894

pISSN:

2602-4837