Cloning the homologue of gene encoding full-length CBF1 transcription factor from Olea europaea L. leaves

Maide Şeker; Şenay Vural Korkut

Makale detayları

Cloning the homologue of gene encoding full-length CBF1 transcription factor from Olea europaea L. leaves

Yazarlar:

Maide Şeker | Şenay Vural Korkut

Yayıncı:

Afyon Kocatepe University

Ana Başlıklar:

Yapısal Biyoloji

Dergi:

Afyon Kocatepe Üniversitesi Fen Ve Mühendislik Bilimleri Dergisi Vol.23 Issue 2, pp.292-299

Belge Tipi:

Makale

Yayın Tarihi:

3/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001288788

pISSN:

2149-3367

Anahtar kelimeler:

Özet:

 One of the most prevalent environmental stresses that affects plants physically and physiologically is the low temperature. Since low temperature adversely affects plant productivity Researchers investigated the molecular processes that regulate the effects of cold stress on plants and discovered many cold responsive genes as well as the control mechanisms that regulate them. CBFDREB C repeat binding factordehydration response element binding factor transcription factors induces the expression of corresponding genes in plants in response to abiotic stress conditions such as cold, drought and salinity. These transcription factors contribute to plant resistance to stress by activating genes in various pathways. Genes encoding CBF transcription factors were first discovered in Arabidopsis thaliana L.. Later, Gene homologs were isolated and cloned from a variety of plants. They belong to the AP2EREBP protein family. In the present study, the full-length cDNA encoding homologue of the transcription factor CBF1 was cloned from Olea europaea L. cv. Gemlik leaves. A 748 bp long cDNA encodes a hypothetical protein of 224 amino acids. BLAST analysis revealed that the CBF1 sequences of Gemlik cultivar and wild olive were nearly identical. A phylogenetic tree was built using Olea europea L. cv. Gemlik CBF1 sequence.

 One of the most prevalent environmental stresses that affects plants physically and physiologically is the low temperature. Since low temperature adversely affects plant productivity Researchers investigated the molecular processes that regulate the effects of cold stress on plants and discovered many cold responsive genes as well as the control mechanisms that regulate them. CBFDREB C repeat binding factordehydration response element binding factor transcription factors induces the expression of corresponding genes in plants in response to abiotic stress conditions such as cold, drought and salinity. These transcription factors contribute to plant resistance to stress by activating genes in various pathways. Genes encoding CBF transcription factors were first discovered in Arabidopsis thaliana L.. Later, Gene homologs were isolated and cloned from a variety of plants. They belong to the AP2EREBP protein family. In the present study, the full-length cDNA encoding homologue of the transcription factor CBF1 was cloned from Olea europaea L. cv. Gemlik leaves. A 748 bp long cDNA encodes a hypothetical protein of 224 amino acids. BLAST analysis revealed that the CBF1 sequences of Gemlik cultivar and wild olive were nearly identical. A phylogenetic tree was built using Olea europea L. cv. Gemlik CBF1 sequence.

 One of the most prevalent environmental stresses that affects plants physically and physiologically is the low temperature. Since low temperature adversely affects plant productivity Researchers investigated the molecular processes that regulate the effects of cold stress on plants and discovered many cold responsive genes as well as the control mechanisms that regulate them. CBFDREB C repeat binding factordehydration response element binding factor transcription factors induces the expression of corresponding genes in plants in response to abiotic stress conditions such as cold, drought and salinity. These transcription factors contribute to plant resistance to stress by activating genes in various pathways. Genes encoding CBF transcription factors were first discovered in Arabidopsis thaliana L.. Later, Gene homologs were isolated and cloned from a variety of plants. They belong to the AP2EREBP protein family. In the present study, the full-length cDNA encoding homologue of the transcription factor CBF1 was cloned from Olea europaea L. cv. Gemlik leaves. A 748 bp long cDNA encodes a hypothetical protein of 224 amino acids. BLAST analysis revealed that the CBF1 sequences of Gemlik cultivar and wild olive were nearly identical. A phylogenetic tree was built using Olea europea L. cv. Gemlik CBF1 sequence.

 Bitkileri fiziksel ve fizyolojik olarak etkileyen en yaygın çevresel streslerden biri düşük sıcaklıktır. Düşük sıcaklık bitki verimliliğini olumsuz etkilediğinden, araştırmacılar soğuk stresinin bitkiler üzerindeki etkilerini düzenleyen moleküler süreçleri araştırmışlar ve soğuğa duyarlı genlerle birlikte bunların kontrol mekanizmalarını da ortaya çıkarmışlardır. CBFDREB C tekrar bağlama faktörüdehidrasyon yanıt elemanı bağlama faktörü transkripsiyon faktörleri, bitkilerde soğuk, kuraklık ve tuzluluk gibi abiyotik stres koşullarına yanıt olarak ifade edilen genlerin ekspresyonunu indüklemektedir. Bu transkripsiyon faktörleri, çeşitli yolaklardaki genleri aktive ederek strese karşı bitki direncine katkıda bulunurlar. CBF transkripsiyon faktörlerini kodlayan genler ilk olarak Arabidopsis thalianada L. keşfedildi. Daha sonra çeşitli bitkilerden gen homologları klonlandı. CBFDREB transkripsiyon faktörleri AP2EREBP protein ailesi içinde yer alırlar. Bu çalışmada, transkripsiyon faktörü CBF1in homologunu kodlayan tam uzunluktaki cDNA, Olea europaea L. cv. Gemlik yapraklarından klonlanmıştır. 748 bp uzunluğunda cDNA, 224 amino asitlik varsayımsal bir proteini kodlar. BLAST analizi, Gemlik çeşidinin ve yabani zeytinin CBF1 dizilerinin neredeyse aynı olduğunu ortaya çıkardı. Olea europea L. cv Gemlik CBF1 dizisi kullanılarak bir filogenetik ağaç oluşturuldu.

Makale detayları

Cloning the homologue of gene encoding full-length CBF1 transcription factor from Olea europaea L. leaves

Yazarlar:

Maide Şeker | Şenay Vural Korkut

Yayıncı:

Afyon Kocatepe University

Ana Başlıklar:

Yapısal Biyoloji

Dergi:

Afyon Kocatepe Üniversitesi Fen Ve Mühendislik Bilimleri Dergisi Vol.23 Issue 2, pp.292-299

Belge Tipi:

Makale

Yayın Tarihi:

3/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001288788

pISSN:

2149-3367

CBF/DREB

Olea europaea L

Gene Cloning

DNA Sequencing

Phylogenetic analysisCBF/DREB

Olea europaea L

Gen Klonlama

DNA Dizileme

Filogenetik Analiz

Sayı

Makale detayları

Yazarlar: Maide Şeker | Şenay Vural Korkut

Yayıncı: Afyon Kocatepe University

Ana Başlıklar: Yapısal Biyoloji

Dergi: Afyon Kocatepe Üniversitesi Fen Ve Mühendislik Bilimleri Dergisi Vol.23 Issue 2, pp.292-299

Belge Tipi: Makale

Yayın Tarihi: 3/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001288788

pISSN: 2149-3367

Sayı

Yazarlar:

Maide Şeker | Şenay Vural Korkut

Yayıncı:

Afyon Kocatepe University

Ana Başlıklar:

Yapısal Biyoloji

Dergi:

Afyon Kocatepe Üniversitesi Fen Ve Mühendislik Bilimleri Dergisi Vol.23 Issue 2, pp.292-299

Belge Tipi:

Makale

Yayın Tarihi:

3/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001288788

pISSN:

2149-3367