Effects of Insulin Lispro on Ram Semen Cryopreservation

Nail Tekin Önder; Taygun Gökdemir; Muhammet Can Kılıç; Savaş Yıldız; Yavuz Öztürkler

Makale detayları

Effects of Insulin Lispro on Ram Semen Cryopreservation

Yazarlar:

Nail Tekin Önder | Taygun Gökdemir | Muhammet Can Kılıç | Savaş Yıldız | Yavuz Öztürkler

Yayıncı:

Afyon Kocatepe University

Ana Başlıklar:

Veteriner Bilimleri (Diğer)

Dergi:

Kocatepe Veteriner Dergisi Vol.16 Issue 3, pp.439-443

Belge Tipi:

Makale

Yayın Tarihi:

9/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001293981

pISSN:

1308-1594

Anahtar kelimeler:

Özet:

 In the present study, it was aimed to evaluate the effects of semen extender with added insulin on ram semen at the post-thaw stage. Semen was collected from 5 Tuj rams five times with an electro-ejaculator. Experimental groups were designated as control group and groups containing 15 IU and 20 IU insulin lispro added semen extenders. At the post-thaw stage, motility and plasma membrane integrity were evaluated by microscopy. Acrosome integrity Fitc-Peanut Agglutinin and mitochondrial membrane potential Rhodamine 123 were assessed by dual staining with propidium iodide PI using flow cytometry. It was found that motility and plasma membrane functional integrity were better preserved in the control group p0.05. Acrosome integrity results were similar between the control and 15 IU insulin groups p0.05, but acrosome integrity was negatively affected in the 20 IU insulin group p0.05. Mitochondrial membrane potential was higher in the group containing 20 IU insulin p0.05. As a result, it was thought that energy metabolism was stimulated in ram semen frozen with insulin-added extenders, and the semen was better preserved than the control group.

 In the present study, it was aimed to evaluate the effects of semen extender with added insulin on ram semen at the post-thaw stage. Semen was collected from 5 Tuj rams five times with an electro-ejaculator. Experimental groups were designated as control group and groups containing 15 IU and 20 IU insulin lispro added semen extenders. At the post-thaw stage, motility and plasma membrane integrity were evaluated by microscopy. Acrosome integrity Fitc-Peanut Agglutinin and mitochondrial membrane potential Rhodamine 123 were assessed by dual staining with propidium iodide PI using flow cytometry. It was found that motility and plasma membrane functional integrity were better preserved in the control group p0.05. Acrosome integrity results were similar between the control and 15 IU insulin groups p0.05, but acrosome integrity was negatively affected in the 20 IU insulin group p0.05. Mitochondrial membrane potential was higher in the group containing 20 IU insulin p0.05. As a result, it was thought that energy metabolism was stimulated in ram semen frozen with insulin-added extenders, and the semen was better preserved than the control group.

 In the present study, it was aimed to evaluate the effects of semen extender with added insulin on ram semen at the post-thaw stage. Semen was collected from 5 Tuj rams five times with an electro-ejaculator. Experimental groups were designated as control group and groups containing 15 IU and 20 IU insulin lispro added semen extenders. At the post-thaw stage, motility and plasma membrane integrity were evaluated by microscopy. Acrosome integrity Fitc-Peanut Agglutinin and mitochondrial membrane potential Rhodamine 123 were assessed by dual staining with propidium iodide PI using flow cytometry. It was found that motility and plasma membrane functional integrity were better preserved in the control group p0.05. Acrosome integrity results were similar between the control and 15 IU insulin groups p0.05, but acrosome integrity was negatively affected in the 20 IU insulin group p0.05. Mitochondrial membrane potential was higher in the group containing 20 IU insulin p0.05. As a result, it was thought that energy metabolism was stimulated in ram semen frozen with insulin-added extenders, and the semen was better preserved than the control group.

 Sunulan çalışmada, insülin ilave edilen sperma sulandırıcısının koç sperması üzerindeki etkilerininin çözdürme sonrası aşamada değerlendirilmesi amaçlanmıştır. Tuj ırkı 5 adet koçtan beş kez elektro-ejakülatör ile sperma alındı. Deneme grupları, kontrol grubu, 15 IU ve 20 IU insulin lispro ilave edilen sperma sulandırıcıları ile oluşturuldu. Çözdürme sonrası, motilite ve plazma membran bütünlüğü mikroskopi ile değerlendirildi. Akrozom bütünlüğü Fitc-Peanut Agglutinin ve mitokondrial membran potansiyeli Rhodamine 123 akış sitometrisi kullanılarak, propidium iodide PI ile ikili boyama yapılarak değerlendirildi. Motilite ve plazma membran fonksiyonel bütünlüğünün kontrol grubuna göre daha iyi korunduğu bulundu p0.05. Akrozom bütünlüğü sonuçları kontrol ve 15 IU insülin grubu arasında benzer olduğu p0.05, ancak 20 IU insulin grubunda akrozom bütünlüğünün olumsuz etkilediği bulundu p0.05. Mitokondrial membran potansiyeli, 20 IU insulin içeren grupta daha yüksek bulundu p0.05. Sonuç olarak, insulin ilave edilmiş sulandırıcılar ile dondurulan koç spermasında enerji metabolizmasının uyarıldığı ve spermanın kontrol grubuna göre daha iyi korunduğu düşünülmüştür.

Makale detayları

Effects of Insulin Lispro on Ram Semen Cryopreservation

Yazarlar:

Nail Tekin Önder | Taygun Gökdemir | Muhammet Can Kılıç | Savaş Yıldız | Yavuz Öztürkler

Yayıncı:

Afyon Kocatepe University

Ana Başlıklar:

Veteriner Bilimleri (Diğer)

Dergi:

Kocatepe Veteriner Dergisi Vol.16 Issue 3, pp.439-443

Belge Tipi:

Makale

Yayın Tarihi:

9/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001293981

pISSN:

1308-1594

Tuj

Ram semen

Insulin

CryopreservationTuj

Koç sperması

İnsülin

Kriyoprezervasyon

Sayı

Makale detayları

Yazarlar: Nail Tekin Önder | Taygun Gökdemir | Muhammet Can Kılıç | Savaş Yıldız | Yavuz Öztürkler

Yayıncı: Afyon Kocatepe University

Ana Başlıklar: Veteriner Bilimleri (Diğer)

Dergi: Kocatepe Veteriner Dergisi Vol.16 Issue 3, pp.439-443

Belge Tipi: Makale

Yayın Tarihi: 9/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001293981

pISSN: 1308-1594

Sayı

Yazarlar:

Nail Tekin Önder | Taygun Gökdemir | Muhammet Can Kılıç | Savaş Yıldız | Yavuz Öztürkler

Yayıncı:

Afyon Kocatepe University

Ana Başlıklar:

Veteriner Bilimleri (Diğer)

Dergi:

Kocatepe Veteriner Dergisi Vol.16 Issue 3, pp.439-443

Belge Tipi:

Makale

Yayın Tarihi:

9/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001293981

pISSN:

1308-1594