Investigation the Role of miR-506 in Metformin-Induced Cell Death Mechanism in MCF-7 and MDA-MB-231 Breast Cancer Cells

Özge Rencuzoğulları; Suraya Qayoumi

Makale detayları

Investigation the Role of miR-506 in Metformin-Induced Cell Death Mechanism in MCF-7 and MDA-MB-231 Breast Cancer Cells

Yazarlar:

Özge Rencuzoğulları | Suraya Qayoumi

Yayıncı:

Manisa Celal Bayar University

Ana Başlıklar:

Nanokimya

Dergi:

Celal Bayar Üniversitesi Fen Bilimleri Dergisi Vol.20 Issue 3, pp.1-9

Belge Tipi:

Makale

Yayın Tarihi:

9/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001328592

pISSN:

1305-130X

Anahtar kelimeler:

Özet:

 Micro RNAs miRNA play a role in basic cellular processes such as cell growth, development, cell cycle and apoptosis by affecting gene expression. Abnormal regulation or changes in expression of miRNAs can be observed in many diseases, especially cancer. Therefore, miRNAs are also being investigated as potential therapeutic targets. It has been suggested that miR-506 may be expressed at low levels in various types of cancer and this may contribute to cancer development. In this study, the effect of miR-506 on the sensitivity of breast cancer cells to metformin was evaluated in terms of its effect on cell survival and apoptotic mechanism. Metformin caused a dose-dependent decrease in cell viability and induced loss of mitochondrial membrane potential in MCF-7 cells while MDA-MB-231 cells were more resistant. The colony formation and migration potential of both cell lines with increased miR-506 expression were significantly suppressed after metformin treatment. Additionally, apoptotic cell death triggered by metformin was induced in both cell lines when miR-506 expression was increased. In conclusion, miR-506 acts as a tumor suppressor in MCF-7 and MDA-MB-231 cells and increases their sensitivity to metformin, indicating the potential of miR-506 to be an important therapeutic target in future studies.

 Micro RNAs miRNA play a role in basic cellular processes such as cell growth, development, cell cycle and apoptosis by affecting gene expression. Abnormal regulation or changes in expression of miRNAs can be observed in many diseases, especially cancer. Therefore, miRNAs are also being investigated as potential therapeutic targets. It has been suggested that miR-506 may be expressed at low levels in various types of cancer and this may contribute to cancer development. In this study, the effect of miR-506 on the sensitivity of breast cancer cells to metformin was evaluated in terms of its effect on cell survival and apoptotic mechanism. Metformin caused a dose-dependent decrease in cell viability and induced loss of mitochondrial membrane potential in MCF-7 cells while MDA-MB-231 cells were more resistant. The colony formation and migration potential of both cell lines with increased miR-506 expression were significantly suppressed after metformin treatment. Additionally, apoptotic cell death triggered by metformin was induced in both cell lines when miR-506 expression was increased. In conclusion, miR-506 acts as a tumor suppressor in MCF-7 and MDA-MB-231 cells and increases their sensitivity to metformin, indicating the potential of miR-506 to be an important therapeutic target in future studies.

 Micro RNAs miRNA play a role in basic cellular processes such as cell growth, development, cell cycle and apoptosis by affecting gene expression. Abnormal regulation or changes in expression of miRNAs can be observed in many diseases, especially cancer. Therefore, miRNAs are also being investigated as potential therapeutic targets. It has been suggested that miR-506 may be expressed at low levels in various types of cancer and this may contribute to cancer development. In this study, the effect of miR-506 on the sensitivity of breast cancer cells to metformin was evaluated in terms of its effect on cell survival and apoptotic mechanism. Metformin caused a dose-dependent decrease in cell viability and induced loss of mitochondrial membrane potential in MCF-7 cells while MDA-MB-231 cells were more resistant. The colony formation and migration potential of both cell lines with increased miR-506 expression were significantly suppressed after metformin treatment. Additionally, apoptotic cell death triggered by metformin was induced in both cell lines when miR-506 expression was increased. In conclusion, miR-506 acts as a tumor suppressor in MCF-7 and MDA-MB-231 cells and increases their sensitivity to metformin, indicating the potential of miR-506 to be an important therapeutic target in future studies.

 Micro RNAs miRNA play a role in basic cellular processes such as cell growth, development, cell cycle and apoptosis by affecting gene expression. Abnormal regulation or changes in expression of miRNAs can be observed in many diseases, especially cancer. Therefore, miRNAs are also being investigated as potential therapeutic targets. It has been suggested that miR-506 may be expressed at low levels in various types of cancer and this may contribute to cancer development. In this study, the effect of miR-506 on the sensitivity of breast cancer cells to metformin was evaluated in terms of its effect on cell survival and apoptotic mechanism. Metformin caused a dose-dependent decrease in cell viability and induced loss of mitochondrial membrane potential in MCF-7 cells while MDA-MB-231 cells were more resistant. The colony formation and migration potential of both cell lines with increased miR-506 expression were significantly suppressed after metformin treatment. Additionally, apoptotic cell death triggered by metformin was induced in both cell lines when miR-506 expression was increased. In conclusion, miR-506 acts as a tumor suppressor in MCF-7 and MDA-MB-231 cells and increases their sensitivity to metformin, indicating the potential of miR-506 to be an important therapeutic target in future studies.

Makale detayları

Investigation the Role of miR-506 in Metformin-Induced Cell Death Mechanism in MCF-7 and MDA-MB-231 Breast Cancer Cells

Yazarlar:

Özge Rencuzoğulları | Suraya Qayoumi

Yayıncı:

Manisa Celal Bayar University

Ana Başlıklar:

Nanokimya

Dergi:

Celal Bayar Üniversitesi Fen Bilimleri Dergisi Vol.20 Issue 3, pp.1-9

Belge Tipi:

Makale

Yayın Tarihi:

9/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001328592

pISSN:

1305-130X

Apoptotic cell death

Breast cancer

Metformin

miR-506

Sayı

Makale detayları

Investigation the Role of miR-506 in Metformin-Induced Cell Death Mechanism in MCF-7 and MDA-MB-231 Breast Cancer Cells

Yazarlar: Özge Rencuzoğulları | Suraya Qayoumi

Yayıncı: Manisa Celal Bayar University

Ana Başlıklar: Nanokimya

Dergi: Celal Bayar Üniversitesi Fen Bilimleri Dergisi Vol.20 Issue 3, pp.1-9

Belge Tipi: Makale

Yayın Tarihi: 9/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001328592

pISSN: 1305-130X

Apoptotic cell death

Breast cancer

Metformin

miR-506

Sayı

Yazarlar:

Özge Rencuzoğulları | Suraya Qayoumi

Yayıncı:

Manisa Celal Bayar University

Ana Başlıklar:

Nanokimya

Dergi:

Celal Bayar Üniversitesi Fen Bilimleri Dergisi Vol.20 Issue 3, pp.1-9

Belge Tipi:

Makale

Yayın Tarihi:

9/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001328592

pISSN:

1305-130X