The Effect of Carob Aqueous Extract on Oxidative Stress, Proinflammatory Cytokines, Glucose and Lipid Concentrations in the Blood of Diabetic Rats.

Mehmet Talha Avşar; Abdullah Eryavuz

Makale detayları

The Effect of Carob Aqueous Extract on Oxidative Stress, Proinflammatory Cytokines, Glucose and Lipid Concentrations in the Blood of Diabetic Rats.

Yazarlar:

Mehmet Talha Avşar | Abdullah Eryavuz

Yayıncı:

Afyon Kocatepe University

Ana Başlıklar:

Veteriner Anatomi ve Fizyoloji

Dergi:

Kocatepe Veteriner Dergisi Vol.18 Issue 3, pp.192-199

Belge Tipi:

Makale

Yayın Tarihi:

3/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001354167

pISSN:

1308-1594

Anahtar kelimeler:

Özet:

 Herbal treatments offer potential benefits in controlling blood glucose levels and preventing diabetes-related complications. This study was conducted to determine the effects of the application of carob fruit aqueous extract prepared by ultrasound-assisted extraction UAE method on oxidative stress, glycemic level, proinflammatory cytokines and lipid profile levels in the blood of rats with experimental diabetes induced by streptozotocin-nicotinamide STZ-NA model. Forty male Wistar Albino 200-250 g live weight animals used in the study were divided into four groups as normal control, diabetic control, normal group given 200 mg.kg-1 carob aqueous extract and diabetic group given 200 mg.kg-1 carob aqueous extract. At the end of the 21-day study, plasma glucose, hemoglobin A1C HbA1c, insulin, homeostatic model assessment of insulin resistance HOMA-IR, malondialdehyde MDA, reduced glutathione GSH, proinflammatory cytokines interleukin 1-beta IL-1β, tumor necrosis factor-alpha TNF-α, interleukin-6 IL-6, total cholesterol, triglyceride, leptin and 25-hydroxy vitamin D levels were measured in blood samples taken from all animals. Carob aqueous extract treatment did not cause a significant decrease in high glucose, HbA1c and HOMA-IR values caused by diabetes. The blood insulin levels were not affected by the treatments. Diabetes increased MDA, IL-6 and triglyceride levels p0.05, while carob aqueous extract treatment to diabetic animals did not affect these parameters. However, carob aqueous extract treatment to diabetic animals increased GSH levels p0.05. The treatments had no effect on IL-1β, TNF-α, total cholesterol and leptin levels. Conclusively, the carob fruit aqueous extract treatment did not have an effect on controlling diabetes-related hyperglycemia.

 Herbal treatments offer potential benefits in controlling blood glucose levels and preventing diabetes-related complications. This study was conducted to determine the effects of the application of carob fruit aqueous extract prepared by ultrasound-assisted extraction UAE method on oxidative stress, glycemic level, proinflammatory cytokines and lipid profile levels in the blood of rats with experimental diabetes induced by streptozotocin-nicotinamide STZ-NA model. Forty male Wistar Albino 200-250 g live weight animals used in the study were divided into four groups as normal control, diabetic control, normal group given 200 mg.kg-1 carob aqueous extract and diabetic group given 200 mg.kg-1 carob aqueous extract. At the end of the 21-day study, plasma glucose, hemoglobin A1C HbA1c, insulin, homeostatic model assessment of insulin resistance HOMA-IR, malondialdehyde MDA, reduced glutathione GSH, proinflammatory cytokines interleukin 1-beta IL-1β, tumor necrosis factor-alpha TNF-α, interleukin-6 IL-6, total cholesterol, triglyceride, leptin and 25-hydroxy vitamin D levels were measured in blood samples taken from all animals. Carob aqueous extract treatment did not cause a significant decrease in high glucose, HbA1c and HOMA-IR values caused by diabetes. The blood insulin levels were not affected by the treatments. Diabetes increased MDA, IL-6 and triglyceride levels p0.05, while carob aqueous extract treatment to diabetic animals did not affect these parameters. However, carob aqueous extract treatment to diabetic animals increased GSH levels p0.05. The treatments had no effect on IL-1β, TNF-α, total cholesterol and leptin levels. Conclusively, the carob fruit aqueous extract treatment did not have an effect on controlling diabetes-related hyperglycemia.

 Herbal treatments offer potential benefits in controlling blood glucose levels and preventing diabetes-related complications. This study was conducted to determine the effects of the application of carob fruit aqueous extract prepared by ultrasound-assisted extraction UAE method on oxidative stress, glycemic level, proinflammatory cytokines and lipid profile levels in the blood of rats with experimental diabetes induced by streptozotocin-nicotinamide STZ-NA model. Forty male Wistar Albino 200-250 g live weight animals used in the study were divided into four groups as normal control, diabetic control, normal group given 200 mg.kg-1 carob aqueous extract and diabetic group given 200 mg.kg-1 carob aqueous extract. At the end of the 21-day study, plasma glucose, hemoglobin A1C HbA1c, insulin, homeostatic model assessment of insulin resistance HOMA-IR, malondialdehyde MDA, reduced glutathione GSH, proinflammatory cytokines interleukin 1-beta IL-1β, tumor necrosis factor-alpha TNF-α, interleukin-6 IL-6, total cholesterol, triglyceride, leptin and 25-hydroxy vitamin D levels were measured in blood samples taken from all animals. Carob aqueous extract treatment did not cause a significant decrease in high glucose, HbA1c and HOMA-IR values caused by diabetes. The blood insulin levels were not affected by the treatments. Diabetes increased MDA, IL-6 and triglyceride levels p0.05, while carob aqueous extract treatment to diabetic animals did not affect these parameters. However, carob aqueous extract treatment to diabetic animals increased GSH levels p0.05. The treatments had no effect on IL-1β, TNF-α, total cholesterol and leptin levels. Conclusively, the carob fruit aqueous extract treatment did not have an effect on controlling diabetes-related hyperglycemia.

 Bitkisel tedaviler, kan glikoz seviyelerinin kontrol altına alınması ve diyabetle ilişkili komplikasyonların önlenmesinde potansiyel faydalar sunmaktadır. Bu çalışmada; streptozotosin-nikotinamid STZ-NA modelle deneysel diyabet oluşturulan sıçanlara ultrasonik-destekli ekstraksiyon UAE yöntemiyle hazırlanmış keçiboynuzu meyvesi sulu ekstraktı uygulamasının kan oksidatif stres, glisemik düzey, proinflamatuar sitokin ve lipit profil düzeylerine etkilerinin belirlenmesi amacıyla yapıldı. Çalışmada 40 adet erkek Wistar Albino 200-250g canlı ağırlık hayvan kullanıldı. Deneydeki hayvanlar normal kontrol, diyabetik kontrol, 200 mgkg keçiboynuzu ekstraktı verilen normal ve 200 mgkg keçiboynuzu ekstraktı verilen diyabetik grup olmak üzere dört gruba ayrıldı. Toplam 21 gün süren çalışmanın sonunda tüm hayvanlardan alınan kan örneklerinde; plazma glikoz, hemoglobin A1C HbA1c, insülin, insülin direncinin homeostatik model değerlendirmesi HOMA-IR, malondialdehid MDA, indirgenmiş glutatyon GSH, proinflamatuar sitokinler interlökin 1-beta IL-1β, tümör nekroz faktörü-alfa TNF-α, interlökin-6 IL-6, total kolesterol, trigliserit, leptin ve 25-hidroksi vitamin D düzeyleri ölçüldü. Keçiboynuzu ekstraktı uygulaması, diyabetin yol açtığı yüksek glikoz, HbA1c ve HOMA-IR değerlerinde önemli bir düşüşe neden olmadı. Uygulamaların kan insülin düzeylerine etkisi olmadı. Diyabet; MDA, IL-6 ve trigliserit düzeylerini artırırken p0.05, keçiboynuzu sulu ekstrakt uygulamasının bu değerlere etkisi gözlenmedi. Bununla birlikte, diyabetli hayvanlara keçiboynuzu sulu ekstraktı verilmesi, GSH düzeylerini yükseltmiştir p0.05. Uygulamaların IL-1β, TNF-α, total kolesterol ve leptin düzeylerine etkisi bulunmadı. Sonuç olarak, keçiboynuzu meyvesi sulu ekstraktının, diyabete bağlı hiperglisemiyi kontrol etmede etkinliğinin bulunmadığı kanaatine varıldı.

Makale detayları

The Effect of Carob Aqueous Extract on Oxidative Stress, Proinflammatory Cytokines, Glucose and Lipid Concentrations in the Blood of Diabetic Rats.

Yazarlar:

Mehmet Talha Avşar | Abdullah Eryavuz

Yayıncı:

Afyon Kocatepe University

Ana Başlıklar:

Veteriner Anatomi ve Fizyoloji

Dergi:

Kocatepe Veteriner Dergisi Vol.18 Issue 3, pp.192-199

Belge Tipi:

Makale

Yayın Tarihi:

3/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001354167

pISSN:

1308-1594

Diabetes

oxidative stress

hyperglycemia

dyslipidemia

Carob aqueous extractDiyabet

Keçiboynuzu sulu ekstraktı

oksidatif stres

hiperglisemi

dislipidemi

Sayı

Makale detayları

Yazarlar: Mehmet Talha Avşar | Abdullah Eryavuz

Yayıncı: Afyon Kocatepe University

Ana Başlıklar: Veteriner Anatomi ve Fizyoloji

Dergi: Kocatepe Veteriner Dergisi Vol.18 Issue 3, pp.192-199

Belge Tipi: Makale

Yayın Tarihi: 3/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001354167

pISSN: 1308-1594

Sayı

Yazarlar:

Mehmet Talha Avşar | Abdullah Eryavuz

Yayıncı:

Afyon Kocatepe University

Ana Başlıklar:

Veteriner Anatomi ve Fizyoloji

Dergi:

Kocatepe Veteriner Dergisi Vol.18 Issue 3, pp.192-199

Belge Tipi:

Makale

Yayın Tarihi:

3/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001354167

pISSN:

1308-1594