Antioxidant capacity, total phenolic content, and in vitro cytotoxic and oxidative effects of the methanol extract of Dunaliella sp. against breast cancer cells

Muazzez Derya Andeden; Pınar Altın Çelik; Enver Ersoy Andeden; Şahlan Öztürk; Hamiyet Altuntaş

Makale detayları

Antioxidant capacity, total phenolic content, and in vitro cytotoxic and oxidative effects of the methanol extract of Dunaliella sp. against breast cancer cells

Yazarlar:

Muazzez Derya Andeden | Pınar Altın Çelik | Enver Ersoy Andeden | Şahlan Öztürk | Hamiyet Altuntaş

Yayıncı:

İzzet KARA

Ana Başlıklar:

Biochemistry and Cell Biology (Other) | Pharmaceutical Toxicology | Natural Products and Bioactive Compounds

Dergi:

International Journal of Secondary Metabolite Vol.12 Issue 3, pp.537-547

Belge Tipi:

Makale

Yayın Tarihi:

4/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001350179

pISSN:

2148-6905

Anahtar kelimeler:

Özet:

 Microalgae are rich sources of essential biomolecules, including proteins, pigments, and a diverse array of secondary metabolites such as carotenoids, polyphenols, sterols, polyunsaturated fatty acids, and polysaccharides, which they secrete at various growth stages. Owing to this property, microalgae are gaining significant research interest as natural product producers with cell-protective potential and minimal or no side effects compared to synthetic drugs. This study explores the potential of the methanol extract of Dunaliella sp. mD as an in vitro therapeutic agent for breast cancer. The antioxidant activity and total phenolic content of mD were assessed using the 2,2-diphenyl-1-picrylhydrazyl DPPH and Folin-Ciocalteu methods. Additionally, its cytotoxic and oxidative effects were evaluated in vitro. mD exhibited a radical scavenging activity RSA of 40.27, 1.8 below the expected value, and a total phenolic content of 25.4 mg GAEg dry extract. The MTT cell viability assay further indicated that this microalgal extract has cytotoxic potential against breast cancer cells. The IC50 values were 768 and 728 µgmL for MCF-7 and MDA-MB-231 cells, respectively. While the use of H2O2 alone led to an increase in ROS production in the cells, treatment with mD reduced ROS production. According to the current study, mD could be an interesting candidate for the synthesis of drugs and nutraceuticals for the treatment of breast cancer.

 Microalgae are rich sources of essential biomolecules, including proteins, pigments, and a diverse array of secondary metabolites such as carotenoids, polyphenols, sterols, polyunsaturated fatty acids, and polysaccharides, which they secrete at various growth stages. Owing to this property, microalgae are gaining significant research interest as natural product producers with cell-protective potential and minimal or no side effects compared to synthetic drugs. This study explores the potential of the methanol extract of Dunaliella sp. mD as an in vitro therapeutic agent for breast cancer. The antioxidant activity and total phenolic content of mD were assessed using the 2,2-diphenyl-1-picrylhydrazyl DPPH and Folin-Ciocalteu methods. Additionally, its cytotoxic and oxidative effects were evaluated in vitro. mD exhibited a radical scavenging activity RSA of 40.27, 1.8 below the expected value, and a total phenolic content of 25.4 mg GAEg dry extract. The MTT cell viability assay further indicated that this microalgal extract has cytotoxic potential against breast cancer cells. The IC50 values were 768 and 728 µgmL for MCF-7 and MDA-MB-231 cells, respectively. While the use of H2O2 alone led to an increase in ROS production in the cells, treatment with mD reduced ROS production. According to the current study, mD could be an interesting candidate for the synthesis of drugs and nutraceuticals for the treatment of breast cancer.

 Microalgae are rich sources of essential biomolecules, including proteins, pigments, and a diverse array of secondary metabolites such as carotenoids, polyphenols, sterols, polyunsaturated fatty acids, and polysaccharides, which they secrete at various growth stages. Owing to this property, microalgae are gaining significant research interest as natural product producers with cell-protective potential and minimal or no side effects compared to synthetic drugs. This study explores the potential of the methanol extract of Dunaliella sp. mD as an in vitro therapeutic agent for breast cancer. The antioxidant activity and total phenolic content of mD were assessed using the 2,2-diphenyl-1-picrylhydrazyl DPPH and Folin-Ciocalteu methods. Additionally, its cytotoxic and oxidative effects were evaluated in vitro. mD exhibited a radical scavenging activity RSA of 40.27, 1.8 below the expected value, and a total phenolic content of 25.4 mg GAEg dry extract. The MTT cell viability assay further indicated that this microalgal extract has cytotoxic potential against breast cancer cells. The IC50 values were 768 and 728 µgmL for MCF-7 and MDA-MB-231 cells, respectively. While the use of H2O2 alone led to an increase in ROS production in the cells, treatment with mD reduced ROS production. According to the current study, mD could be an interesting candidate for the synthesis of drugs and nutraceuticals for the treatment of breast cancer.

 Microalgae are rich sources of essential biomolecules, including proteins, pigments, and a diverse array of secondary metabolites such as carotenoids, polyphenols, sterols, polyunsaturated fatty acids, and polysaccharides, which they secrete at various growth stages. Owing to this property, microalgae are gaining significant research interest as natural product producers with cell-protective potential and minimal or no side effects compared to synthetic drugs. This study explores the potential of the methanol extract of Dunaliella sp. mD as an in vitro therapeutic agent for breast cancer. The antioxidant activity and total phenolic content of mD were assessed using the 2,2-diphenyl-1-picrylhydrazyl DPPH and Folin-Ciocalteu methods. Additionally, its cytotoxic and oxidative effects were evaluated in vitro. mD exhibited a radical scavenging activity RSA of 40.27, 1.8 below the expected value, and a total phenolic content of 25.4 mg GAEg dry extract. The MTT cell viability assay further indicated that this microalgal extract has cytotoxic potential against breast cancer cells. The IC50 values were 768 and 728 µgmL for MCF-7 and MDA-MB-231 cells, respectively. While the use of H2O2 alone led to an increase in ROS production in the cells, treatment with mD reduced ROS production. According to the current study, mD could be an interesting candidate for the synthesis of drugs and nutraceuticals for the treatment of breast cancer.

Makale detayları

Antioxidant capacity, total phenolic content, and in vitro cytotoxic and oxidative effects of the methanol extract of Dunaliella sp. against breast cancer cells

Yazarlar:

Muazzez Derya Andeden | Pınar Altın Çelik | Enver Ersoy Andeden | Şahlan Öztürk | Hamiyet Altuntaş

Yayıncı:

İzzet KARA

Ana Başlıklar:

Biochemistry and Cell Biology (Other) | Pharmaceutical Toxicology | Natural Products and Bioactive Compounds

Dergi:

International Journal of Secondary Metabolite Vol.12 Issue 3, pp.537-547

Belge Tipi:

Makale

Yayın Tarihi:

4/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001350179

pISSN:

2148-6905

Anticancer

Cytotoxicity

Dunaliella

Lake Tuz

Reactive oxygen species

Secondary metabolitesAnticancer

Cytotoxicity

Dunaliella

Lake Tuz

Reactive oxygen species

Secondary metabolites

Sayı

Makale detayları

Yazarlar: Muazzez Derya Andeden | Pınar Altın Çelik | Enver Ersoy Andeden | Şahlan Öztürk | Hamiyet Altuntaş

Yayıncı: İzzet KARA

Ana Başlıklar: Biochemistry and Cell Biology (Other) | Pharmaceutical Toxicology | Natural Products and Bioactive Compounds

Dergi: International Journal of Secondary Metabolite Vol.12 Issue 3, pp.537-547

Belge Tipi: Makale

Yayın Tarihi: 4/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001350179

pISSN: 2148-6905

Sayı

Yazarlar:

Muazzez Derya Andeden | Pınar Altın Çelik | Enver Ersoy Andeden | Şahlan Öztürk | Hamiyet Altuntaş

Yayıncı:

İzzet KARA

Ana Başlıklar:

Biochemistry and Cell Biology (Other) | Pharmaceutical Toxicology | Natural Products and Bioactive Compounds

Dergi:

International Journal of Secondary Metabolite Vol.12 Issue 3, pp.537-547

Belge Tipi:

Makale

Yayın Tarihi:

4/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001350179

pISSN:

2148-6905