THREE NEW BIS(ACYLHYDRAZONE) MONONUCLEAR TRANSITION METAL COMPLEXES: SYNTHESIS, CHARACTERIZATIONS AND DNA INTERACTION STUDIES

Sultan Kıncal; Tolga Göktürk; Cansu Topkaya; Ramazan Güp

Makale detayları

THREE NEW BIS(ACYLHYDRAZONE) MONONUCLEAR TRANSITION METAL COMPLEXES: SYNTHESIS, CHARACTERIZATIONS AND DNA INTERACTION STUDIES

Yazarlar:

Sultan Kıncal | Tolga Göktürk | Cansu Topkaya | Ramazan Güp

Yayıncı:

Mugla Sitki Kocman University

Ana Başlıklar:

Analitik Kimya (Diğer)

Dergi:

Mugla Journal of Science and Technology Vol.9 Issue 2, pp.36-45

Belge Tipi:

Makale

Yayın Tarihi:

12/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001305085

pISSN:

2149-3596

Anahtar kelimeler:

Özet:

 In recent years, studies on the design and synthesis of artificial nucleases with biological, structural and coordination properties have been increasing. With this study, mononuclear complex compounds were obtained with CuII, NiII and ZnII transition metal ion salts of bisacylhydrazone ligand, which has the potential to show biological activity and has revolving atoms such as N and O in its structure. The characteristic structures of the obtained complex compounds were elucidated using various techniques such as FTIR, UV-Visible, 1H-NMR, TGA, elemental analysis and magnetic susceptibility. The DNA-binding activities of potential artificial metallonucleases, whose structures were elucidated, were investigated using the UV-visible absorption titration method. Additionally, their DNA-cleavge activities were analyzed using the agarose gel electrophoresis method. The DNA binding mode and cleavage mechanisms were determined as part of this study.. In line with the results obtained, it was determined that the binding mode between DNA and the complexes is a noncovalent interaction. It has been demonstrated that the compounds cleavage DNA both oxidatively and hydrolytically. It has been revealed that the radicals involved in DNA cleavage activity are H2O2, superoxide and hydroxyl radicals. It was revealed that the compound with the highest cleavage and bonding interaction was the CuII complex, followed by NiII and ZnII complexes.

 In recent years, studies on the design and synthesis of artificial nucleases with biological, structural and coordination properties have been increasing. With this study, mononuclear complex compounds were obtained with CuII, NiII and ZnII transition metal ion salts of bisacylhydrazone ligand, which has the potential to show biological activity and has revolving atoms such as N and O in its structure. The characteristic structures of the obtained complex compounds were elucidated using various techniques such as FTIR, UV-Visible, 1H-NMR, TGA, elemental analysis and magnetic susceptibility. The DNA-binding activities of potential artificial metallonucleases, whose structures were elucidated, were investigated using the UV-visible absorption titration method. Additionally, their DNA-cleavge activities were analyzed using the agarose gel electrophoresis method. The DNA binding mode and cleavage mechanisms were determined as part of this study.. In line with the results obtained, it was determined that the binding mode between DNA and the complexes is a noncovalent interaction. It has been demonstrated that the compounds cleavage DNA both oxidatively and hydrolytically. It has been revealed that the radicals involved in DNA cleavage activity are H2O2, superoxide and hydroxyl radicals. It was revealed that the compound with the highest cleavage and bonding interaction was the CuII complex, followed by NiII and ZnII complexes.

 In recent years, studies on the design and synthesis of artificial nucleases with biological, structural and coordination properties have been increasing. With this study, mononuclear complex compounds were obtained with CuII, NiII and ZnII transition metal ion salts of bisacylhydrazone ligand, which has the potential to show biological activity and has revolving atoms such as N and O in its structure. The characteristic structures of the obtained complex compounds were elucidated using various techniques such as FTIR, UV-Visible, 1H-NMR, TGA, elemental analysis and magnetic susceptibility. The DNA-binding activities of potential artificial metallonucleases, whose structures were elucidated, were investigated using the UV-visible absorption titration method. Additionally, their DNA-cleavge activities were analyzed using the agarose gel electrophoresis method. The DNA binding mode and cleavage mechanisms were determined as part of this study.. In line with the results obtained, it was determined that the binding mode between DNA and the complexes is a noncovalent interaction. It has been demonstrated that the compounds cleavage DNA both oxidatively and hydrolytically. It has been revealed that the radicals involved in DNA cleavage activity are H2O2, superoxide and hydroxyl radicals. It was revealed that the compound with the highest cleavage and bonding interaction was the CuII complex, followed by NiII and ZnII complexes.

 Son yıllarda biyolojik açıdan aktif ve yapısında metal içeren yapay nükleazların tasarlanması ve sentezine yönelik çalışmalara giderek çoğalmaktadır. Gerçekleştirilen bu çalışma ile biyolojik aktivite gösterme potansiyeli olan ve yapısında N ve O gibi dönor atomlar bulunduran bisaçilhidrazonligandın CuII, NiII ve ZnII geçiş metal iyon tuzlarıyla mononükleer kompleks bileşikleri elde edilmiştir. Elde edilen kompleks bileşiklerin karakteristik yapıları, FTIR, UV-Görünür, 1H-NMR, TGA, elemental analiz ve magnetic süssebilite gibi çeşitli teknikler kullanılarak aydınlatılmıştır. Yapıları aydınlatılan potansiyel yapay metallonükleazların UV-vis. adsorbsiyon titrasyon metodu ile DNA-bağlanma, agaroz jel elektroforez yöntemi ile de DNA-kesme aktiviteleri incelenmiştir. DNA bağlanma modu ve kesme mekanizmaları belirlenmiştir. Elde edilen sonuçlar doğrultusunda, DNA ile kompleksler arasındaki bağlanma modunun nonkovalent bir etkileşim olduğu belirlenmiştir. Bileşiklerin hem oksidadif hem de hidrolitik olarak DNA yı kestiği ortaya koyulmuştur. DNA kesme aktivitesinde rol alan radikallerin H2O2, superoksit ve hidroksil radikalleri olduğu ortaya çıkarılmıştır. Kesme ve bağlanma etkileşiminin en yüksek olduğu bileşiğin CuII kompleksi olduğu ve bu sıralamayı NiII ve ZnII komplekslerinin izlediği ortaya konmuştur.

Makale detayları

THREE NEW BIS(ACYLHYDRAZONE) MONONUCLEAR TRANSITION METAL COMPLEXES: SYNTHESIS, CHARACTERIZATIONS AND DNA INTERACTION STUDIES

Yazarlar:

Sultan Kıncal | Tolga Göktürk | Cansu Topkaya | Ramazan Güp

Yayıncı:

Mugla Sitki Kocman University

Ana Başlıklar:

Analitik Kimya (Diğer)

Dergi:

Mugla Journal of Science and Technology Vol.9 Issue 2, pp.36-45

Belge Tipi:

Makale

Yayın Tarihi:

12/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001305085

pISSN:

2149-3596

bis(acylhydrazones)

metal complexes

DNA interactionsbis(açilhidrazon)lar

metal kompleksler

DNA etkileşim

Sayı

Makale detayları

THREE NEW BIS(ACYLHYDRAZONE) MONONUCLEAR TRANSITION METAL COMPLEXES: SYNTHESIS, CHARACTERIZATIONS AND DNA INTERACTION STUDIES

Yazarlar: Sultan Kıncal | Tolga Göktürk | Cansu Topkaya | Ramazan Güp

Yayıncı: Mugla Sitki Kocman University

Ana Başlıklar: Analitik Kimya (Diğer)

Dergi: Mugla Journal of Science and Technology Vol.9 Issue 2, pp.36-45

Belge Tipi: Makale

Yayın Tarihi: 12/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001305085

pISSN: 2149-3596

bis(acylhydrazones)

metal complexes

DNA interactionsbis(açilhidrazon)lar

metal kompleksler

DNA etkileşim

Sayı

Yazarlar:

Sultan Kıncal | Tolga Göktürk | Cansu Topkaya | Ramazan Güp

Yayıncı:

Mugla Sitki Kocman University

Ana Başlıklar:

Analitik Kimya (Diğer)

Dergi:

Mugla Journal of Science and Technology Vol.9 Issue 2, pp.36-45

Belge Tipi:

Makale

Yayın Tarihi:

12/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001305085

pISSN:

2149-3596