The effect of multiflora honey from Southeastern Anatolia on pro-inflammatory and pro-apoptotic pathways in MDA-MB-231 cell line

Dilan Erbaş; Aslı Özkızılcık Koçyiğit; Fikriye Fulya Kavak; Melek Pehlivan; Tülay Kılıçaslan Ayna

Makale detayları

The effect of multiflora honey from Southeastern Anatolia on pro-inflammatory and pro-apoptotic pathways in MDA-MB-231 cell line

Yazarlar:

Dilan Erbaş | Aslı Özkızılcık Koçyiğit | Fikriye Fulya Kavak | Melek Pehlivan | Tülay Kılıçaslan Ayna

Yayıncı:

Cukurova University

Ana Başlıklar:

Hücresel İmmünoloji | Kanser Hücre Biyolojisi | Kanser Tedavisi (Kemoterapi ve Radyoterapi hariç)

Dergi:

Cukurova Medical Journal Vol.50 Issue 3, pp.635-644

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001357833

pISSN:

2602-3032

Anahtar kelimeler:

Özet:

 Purpose: Honey is increasingly recognized for its medicinal and nutritional benefits, including its anti-bacterial, anti-inflammatory, antioxidant, anti-ulcer, and anti-tumor properties. This study aimed to evaluate the effects of Anatolian honey Southeast Anatolia on the expression of pro-apoptotic genes and chemotactic molecules in breast cancer cells. Materials and Methods: The IC50 concentration of Anatolian honey, determined to be 2.7 wv at 48 hours, was used for treatment. Gene expression analysis was performed using the SYBR Green quantitative reverse transcription polymerase chain reaction qRT-PCR technique. Results: The expression levels of the pro-apoptotic genes Bax and Caspase-3 increased by 6.05-fold and 2.01-fold, respectively, while the chemotactic molecule CCL2 exhibited a 6.45-fold upregulation. In contrast, CCL5 and CX3CR1 showed minimal changes in expression, with fold changes of 1.04 and 1.11, respectively. Anatolian honey promoted apoptosis by increasing Bax and Cas3 expression. Additionally, the elevated CCL2 expression suggests a potential role in recruiting monocytes and facilitating their differentiation into macrophages within the tumor microenvironment. Conclusion: Anatolian honey exhibits pro-apoptotic and immunomodulatory effects in breast cancer cells by upregulating Bax, Cas3, and CCL2 expression. These findings highlight its potential as a complementary therapeutic agent.

 Purpose: Honey is increasingly recognized for its medicinal and nutritional benefits, including its anti-bacterial, anti-inflammatory, antioxidant, anti-ulcer, and anti-tumor properties. This study aimed to evaluate the effects of Anatolian honey Southeast Anatolia on the expression of pro-apoptotic genes and chemotactic molecules in breast cancer cells. Materials and Methods: The IC50 concentration of Anatolian honey, determined to be 2.7 wv at 48 hours, was used for treatment. Gene expression analysis was performed using the SYBR Green quantitative reverse transcription polymerase chain reaction qRT-PCR technique. Results: The expression levels of the pro-apoptotic genes Bax and Caspase-3 increased by 6.05-fold and 2.01-fold, respectively, while the chemotactic molecule CCL2 exhibited a 6.45-fold upregulation. In contrast, CCL5 and CX3CR1 showed minimal changes in expression, with fold changes of 1.04 and 1.11, respectively. Anatolian honey promoted apoptosis by increasing Bax and Cas3 expression. Additionally, the elevated CCL2 expression suggests a potential role in recruiting monocytes and facilitating their differentiation into macrophages within the tumor microenvironment. Conclusion: Anatolian honey exhibits pro-apoptotic and immunomodulatory effects in breast cancer cells by upregulating Bax, Cas3, and CCL2 expression. These findings highlight its potential as a complementary therapeutic agent.

 Purpose: Honey is increasingly recognized for its medicinal and nutritional benefits, including its anti-bacterial, anti-inflammatory, antioxidant, anti-ulcer, and anti-tumor properties. This study aimed to evaluate the effects of Anatolian honey Southeast Anatolia on the expression of pro-apoptotic genes and chemotactic molecules in breast cancer cells. Materials and Methods: The IC50 concentration of Anatolian honey, determined to be 2.7 wv at 48 hours, was used for treatment. Gene expression analysis was performed using the SYBR Green quantitative reverse transcription polymerase chain reaction qRT-PCR technique. Results: The expression levels of the pro-apoptotic genes Bax and Caspase-3 increased by 6.05-fold and 2.01-fold, respectively, while the chemotactic molecule CCL2 exhibited a 6.45-fold upregulation. In contrast, CCL5 and CX3CR1 showed minimal changes in expression, with fold changes of 1.04 and 1.11, respectively. Anatolian honey promoted apoptosis by increasing Bax and Cas3 expression. Additionally, the elevated CCL2 expression suggests a potential role in recruiting monocytes and facilitating their differentiation into macrophages within the tumor microenvironment. Conclusion: Anatolian honey exhibits pro-apoptotic and immunomodulatory effects in breast cancer cells by upregulating Bax, Cas3, and CCL2 expression. These findings highlight its potential as a complementary therapeutic agent.

 Amaç: Bal, anti-bakteriyel, anti-enflamatuvar, antioksidan, anti-ülser ve anti-tümör özellikleri gibi tıbbi ve besinsel faydalarıyla giderek daha fazla ilgi görmektedir. Bu çalışma, Anadolu balının meme kanserinde pro-apoptotik genler ve kemotaktik moleküller üzerindeki etkisini değerlendirmeyi amaçlamıştır. Gereç ve Yöntem: Anadolu balının IC50 konsantrasyonu, 48. saatte 2.7 ağırlıkhacim olarak belirlenmiştir. Gen ekspresyon analizleri, SYBR Green yöntemi ile kantitatif gerçek zamanlı polimeraz zincir reaksiyonu qRT-PCR kullanılarak gerçekleştirilmiştir. Bulgular: Pro-apoptotik genler olan Bax ve Kaspaz 3 Cas3 ile kemotaktik molekül CCL2nin ekspresyon seviyelerinde anlamlı bir artış gözlenmiştir. Buna karşın, CCL5 ve CX3CR1ekspresyon seviyelerinde herhangi bir değişiklik tespit edilmemiştir. Anadolu balı, Bax ve Cas3 gen ekspresyonunu artırarak apoptozu teşvik etmiştir. Ayrıca, CCL2 ekspresyonundaki artış, monositlerin tümör mikroçevresine toplanmasını ve makrofajlara farklılaşmasını kolaylaştırabileceğini göstermektedir. Sonuç: Anadolu balı, meme kanserinde Bax, Cas3 ve CCL2 ekspresyonunu artırarak pro-apoptotik ve immün düzenleyici etkiler göstermektedir. Bu bulgular, Anadolu balının tamamlayıcı bir tedavi ajanı olarak potansiyelini ortaya koymaktadır.

Makale detayları

The effect of multiflora honey from Southeastern Anatolia on pro-inflammatory and pro-apoptotic pathways in MDA-MB-231 cell line

Yazarlar:

Dilan Erbaş | Aslı Özkızılcık Koçyiğit | Fikriye Fulya Kavak | Melek Pehlivan | Tülay Kılıçaslan Ayna

Yayıncı:

Cukurova University

Ana Başlıklar:

Hücresel İmmünoloji | Kanser Hücre Biyolojisi | Kanser Tedavisi (Kemoterapi ve Radyoterapi hariç)

Dergi:

Cukurova Medical Journal Vol.50 Issue 3, pp.635-644

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001357833

pISSN:

2602-3032

honey

chemokine

breast cancer

apoptosis

cell culturebal

kemokin

apoptoz

meme kanseri

hücre kültürü

Sayı

Makale detayları

Yazarlar: Dilan Erbaş | Aslı Özkızılcık Koçyiğit | Fikriye Fulya Kavak | Melek Pehlivan | Tülay Kılıçaslan Ayna

Yayıncı: Cukurova University

Ana Başlıklar: Hücresel İmmünoloji | Kanser Hücre Biyolojisi | Kanser Tedavisi (Kemoterapi ve Radyoterapi hariç)

Dergi: Cukurova Medical Journal Vol.50 Issue 3, pp.635-644

Belge Tipi: Makale

Yayın Tarihi: 9/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001357833

pISSN: 2602-3032

Sayı

Yazarlar:

Dilan Erbaş | Aslı Özkızılcık Koçyiğit | Fikriye Fulya Kavak | Melek Pehlivan | Tülay Kılıçaslan Ayna

Yayıncı:

Cukurova University

Ana Başlıklar:

Hücresel İmmünoloji | Kanser Hücre Biyolojisi | Kanser Tedavisi (Kemoterapi ve Radyoterapi hariç)

Dergi:

Cukurova Medical Journal Vol.50 Issue 3, pp.635-644

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001357833

pISSN:

2602-3032