Evaluation of Pathogens and Antibiotic Resistance Profiles Isolated from Lower Respiratory Tract Samples

Melahat Gürbüz

Makale detayları

Evaluation of Pathogens and Antibiotic Resistance Profiles Isolated from Lower Respiratory Tract Samples

Yazarlar:

Melahat Gürbüz

Yayıncı:

Kocaeli University

Ana Başlıklar:

Tıbbi Mikrobiyoloji

Dergi:

Kocaeli Üniversitesi Sağlık Bilimleri Dergisi Vol.9 Issue 1, pp.14-19

Belge Tipi:

Makale

Yayın Tarihi:

2/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001283745

pISSN:

2149-8571

Anahtar kelimeler:

Özet:

 Objective: In this study, we aimed to guide clinicians in planning empirical treatment and contribute to regional data by presenting bacterial lower respiratory tract infection agents and antibiotic resistance profiles. Methods: All lower respiratory tract samples from patients admitted to our laboratory between January 01 and December 31, 2021 were evaluated retrospectively. Bacterial identification and susceptibility tests were performed using the VITEK 2 automated system and evaluated in accordance with the recommendations of the European Committee on Antimicrobial Susceptibility Testing EUCAST. Results: Significant growth was detected in 461 of 923 lower respiratory tract samples. Antibiotic susceptibility test results of 340 samples were evaluated after excluding the repeated samples of same patient. Gram negative bacteria was isolated in 309 90.9, Staphylococcus aureus in 17 5 and Candida albicans in 14 4.1 of 340 samples. The most frequently isolated Gram-negative bacteria were Pseudomonas aeruginosa 121, 39.2, Acinetobacter baumannii 88, 28.5, Klebsiella pneumoniae 64, 20.7 and Escherichia coli 36, 11.6, respectively. Resistance rates of A. baumannii isolates to carbapenems, piperacillintazobactam, ceftazidime and fluoroquinolones were found to be over 90, and higher resistance was observed in intensive care units ICU than in wards. Conclusion: The increased antibiotic resistance observed in lower respiratory tract infections from hospitalized patients in ICUs, is remarkable. As a result, knowing the causative agents and current resistance profiles is important, especially for hospitalized patients in ICUs, in order to initiate appropriate empirical treatment and to ensure treatment success. The data obtained from our study will guide clinicians in planning empirical treatment.

 Objective: In this study, we aimed to guide clinicians in planning empirical treatment and contribute to regional data by presenting bacterial lower respiratory tract infection agents and antibiotic resistance profiles. Methods: All lower respiratory tract samples from patients admitted to our laboratory between January 01 and December 31, 2021 were evaluated retrospectively. Bacterial identification and susceptibility tests were performed using the VITEK 2 automated system and evaluated in accordance with the recommendations of the European Committee on Antimicrobial Susceptibility Testing EUCAST. Results: Significant growth was detected in 461 of 923 lower respiratory tract samples. Antibiotic susceptibility test results of 340 samples were evaluated after excluding the repeated samples of same patient. Gram negative bacteria was isolated in 309 90.9, Staphylococcus aureus in 17 5 and Candida albicans in 14 4.1 of 340 samples. The most frequently isolated Gram-negative bacteria were Pseudomonas aeruginosa 121, 39.2, Acinetobacter baumannii 88, 28.5, Klebsiella pneumoniae 64, 20.7 and Escherichia coli 36, 11.6, respectively. Resistance rates of A. baumannii isolates to carbapenems, piperacillintazobactam, ceftazidime and fluoroquinolones were found to be over 90, and higher resistance was observed in intensive care units ICU than in wards. Conclusion: The increased antibiotic resistance observed in lower respiratory tract infections from hospitalized patients in ICUs, is remarkable. As a result, knowing the causative agents and current resistance profiles is important, especially for hospitalized patients in ICUs, in order to initiate appropriate empirical treatment and to ensure treatment success. The data obtained from our study will guide clinicians in planning empirical treatment.

 Objective: In this study, we aimed to guide clinicians in planning empirical treatment and contribute to regional data by presenting bacterial lower respiratory tract infection agents and antibiotic resistance profiles. Methods: All lower respiratory tract samples from patients admitted to our laboratory between January 01 and December 31, 2021 were evaluated retrospectively. Bacterial identification and susceptibility tests were performed using the VITEK 2 automated system and evaluated in accordance with the recommendations of the European Committee on Antimicrobial Susceptibility Testing EUCAST. Results: Significant growth was detected in 461 of 923 lower respiratory tract samples. Antibiotic susceptibility test results of 340 samples were evaluated after excluding the repeated samples of same patient. Gram negative bacteria was isolated in 309 90.9, Staphylococcus aureus in 17 5 and Candida albicans in 14 4.1 of 340 samples. The most frequently isolated Gram-negative bacteria were Pseudomonas aeruginosa 121, 39.2, Acinetobacter baumannii 88, 28.5, Klebsiella pneumoniae 64, 20.7 and Escherichia coli 36, 11.6, respectively. Resistance rates of A. baumannii isolates to carbapenems, piperacillintazobactam, ceftazidime and fluoroquinolones were found to be over 90, and higher resistance was observed in intensive care units ICU than in wards. Conclusion: The increased antibiotic resistance observed in lower respiratory tract infections from hospitalized patients in ICUs, is remarkable. As a result, knowing the causative agents and current resistance profiles is important, especially for hospitalized patients in ICUs, in order to initiate appropriate empirical treatment and to ensure treatment success. The data obtained from our study will guide clinicians in planning empirical treatment.

 Amaç: Bu çalışmada hastanemize başvuran hastalardan izole edilen bakteriyel alt solunum yolu enfeksiyonu etkenleri ve antibiyotik direnç profilleri ortaya konularak ampirik tedavinin planlanmasında klinisyenlere yol gösterici olmak ve bölgesel verilere katkı sağlamak amaçlanmaktadır. Yöntem: Hastanemiz farklı birimlerine 01 Ocak  31 Aralık 2021 tarihleri arasında başvuran hastalardan gönderilen alt solunum yoluna ait tüm örnekler retrospektif olarak değerlendirildi. Tespit edilen etkenlerin tanımlama ve duyarlılık testleri VITEK 2 otomatize sistemi kullanılarak yapıldı. Antibiyotik duyarlılıkları ise European Committee on Antimicrobial Susceptibility Testing EUCAST önerileri doğrultusunda değerlendirildi. Bulgular: Toplam 923 alt solunum yolu örneğinden 461inde anlamlı üreme saptandı. Aynı hastaya ait tekrarlayan örnekler ve az sayıda üreyen bakterilerin olduğu örnekler çıkarıldığında kalan 340 örneğe ait antibiyotik duyarlılık test sonuçları değerlendirildi. 340 örnekten 309unda 90,9 Gram negatif bakteriler izole edilirken 17sinde 5 Staphylococcus aureus, 14ünde 4,1 Candida albicans üredi. En sık izole edilen Gram negatif bakteriler sırasıyla Pseudomonas aeruginosa 121, 39,2, Acinetobacter baumannii 88, 28,5, Klebsiella pneumoniae 64, 20,7 ve Escherichia coli 36, 11,6 idi. A. baumannii izolatlarının karbapenem, piperasilintazobaktam, seftazidim ve florokinolon direnç oranları 90ın üzerinde saptandı ve yoğun bakım ünitelerinde YBÜ servislere göre daha yüksek direnç izlendi. Sonuç: Özellikle YBÜlerinde yatan hatan hastalardan izole edilen alt solunum yolu enfeksiyonu etkenlerinde gözlenen artmış antibiyotik direnci dikkat çekicidir. Sonuç olarak solunum yolu enfeksiyonu etkenlerinin ve güncel direnç profillerinin bilinmesi özellikle YBÜleri gibi riskli birimlerde yatan hastalarda, hızla uygun ampirik tedaviye başlanması ve tedavi başarısının sağlanması açısından önemlidir. Çalışmamızda elde ettiğimiz verilerin ampirik tedavinin planlanmasında klinisyenlere yol gösterici olacağı düşünülmektedir.

Makale detayları

Evaluation of Pathogens and Antibiotic Resistance Profiles Isolated from Lower Respiratory Tract Samples

Yazarlar:

Melahat Gürbüz

Yayıncı:

Kocaeli University

Ana Başlıklar:

Tıbbi Mikrobiyoloji

Dergi:

Kocaeli Üniversitesi Sağlık Bilimleri Dergisi Vol.9 Issue 1, pp.14-19

Belge Tipi:

Makale

Yayın Tarihi:

2/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001283745

pISSN:

2149-8571

Lower respiratory tract infections

Antibiotic resistance

Acinetobacter baumannii

Pseudomonas aeruginosa

Alt solunum yolu enfeksiyonları

Antibiyotik direnci

Acinetobacter baumannii

Pseudomonas aeruginosa

Sayı

Makale detayları

Yazarlar: Melahat Gürbüz

Yayıncı: Kocaeli University

Ana Başlıklar: Tıbbi Mikrobiyoloji

Dergi: Kocaeli Üniversitesi Sağlık Bilimleri Dergisi Vol.9 Issue 1, pp.14-19

Belge Tipi: Makale

Yayın Tarihi: 2/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001283745

pISSN: 2149-8571

Sayı

Yazarlar:

Melahat Gürbüz

Yayıncı:

Kocaeli University

Ana Başlıklar:

Tıbbi Mikrobiyoloji

Dergi:

Kocaeli Üniversitesi Sağlık Bilimleri Dergisi Vol.9 Issue 1, pp.14-19

Belge Tipi:

Makale

Yayın Tarihi:

2/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001283745

pISSN:

2149-8571