Yaprağı Yenen Sebzelerdeki Mikroorganizma Türlerinin MALDI-TOF MS (Matris Destekli Lazer Desorpsiyon/İyonizasyon Uçuş Süresi Kütle Spektrometresi) Tekniği Kullanılarak Tanılanması

Aysun UYSUL; Şener KURT; Soner SOYLU; Emine Mine SOYLU; Merve KARA

Makale detayları

Yaprağı Yenen Sebzelerdeki Mikroorganizma Türlerinin MALDI-TOF MS (Matris Destekli Lazer Desorpsiyon/İyonizasyon Uçuş Süresi Kütle Spektrometresi) Tekniği Kullanılarak Tanılanması

Yazarlar:

Aysun UYSUL | Şener KURT | Soner SOYLU | Emine Mine SOYLU | Merve KARA

Yayıncı:

Van Yuzuncu Yıl University

Ana Başlıklar:

Üretim

Dergi:

Yuzuncu Yıl University Journal of Agricultural Sciences 2019 Vol.29 Issue 4, pp.595-603

Belge Tipi:

Makale

Yayın Tarihi:

12/2019

Belge Dili:

Türkçe

Document ID (Org No.):

2000651044

Sıklık:

Üç Aylık

eISSN:

1308-7584

pISSN:

1308-7576

Özet:

 


The
foodborne fungal and bacterial pathogens are increasingly associated with fresh
fruit and vegetables. Bacterial colonization of fresh produce plants can occur
to high levels on the external tissue but bacteria have also been detected
within plant tissue. Leafy vegetables such as lettuce, spinach, cabbage,
rocket, mint, parsley, dill, and leek were collected from different vegetable
markets in the counties of Defne and Antakya of Hatay province. Growing media potato
dextrose agar, nutrient agar and blood agar media were used for microbiological
analysis. Their identification were performed based on phenotypic characters
and matrix-assisted laser desorption/ionization time-of-flight mass
spectrometry (MALDI-TOF MS) technique. According to the results of MALDI-TOF MS
analysis, when the distribution of microorganisms were examined, the most
bacterial species were identified with 60 species at the species level,
followed by 4 fungi and 1 yeast.   The
most microorganism species were obtained from lettuce samples.   When the distribution of species level is
examined; firstly, 9 species with Pseudomonas,
5 species with Lactobacillus, 4
species with Acinetobacter and 2
species with Klebsiella, Citrobacter,
Enterobacter, Lysinibacillus were obtained and Pantoea one species Bacillus,
one species Escherichia coli, one
species followed by Exiguobacterium genera.
When the colony density of microorganism species were examined, the most
intensive colony was determined in the species belonging to the genus Pseudomonas and Acinetobacter. At least colony density was observed in fungi. In
result, Salmonella sp, Klebsiella pneumoniae, Escherichia coli,
Bacillus cereus, Klebsiella oxytoca species were found to be very dangerous
species for human health.

.

Makale detayları

Yaprağı Yenen Sebzelerdeki Mikroorganizma Türlerinin MALDI-TOF MS (Matris Destekli Lazer Desorpsiyon/İyonizasyon Uçuş Süresi Kütle Spektrometresi) Tekniği Kullanılarak Tanılanması

Yazarlar: Aysun UYSUL | Şener KURT | Soner SOYLU | Emine Mine SOYLU | Merve KARA

Yayıncı: Van Yuzuncu Yıl University

Ana Başlıklar: Üretim

Dergi: Yuzuncu Yıl University Journal of Agricultural Sciences 2019 Vol.29 Issue 4, pp.595-603

Belge Tipi: Makale

Yayın Tarihi: 12/2019

Belge Dili: Türkçe

Document ID (Org No.): 2000651044

Sıklık: Üç Aylık

eISSN: 1308-7584

pISSN: 1308-7576

Sayı

Yazarlar:

Aysun UYSUL | Şener KURT | Soner SOYLU | Emine Mine SOYLU | Merve KARA

Yayıncı:

Van Yuzuncu Yıl University

Ana Başlıklar:

Üretim

Dergi:

Yuzuncu Yıl University Journal of Agricultural Sciences 2019 Vol.29 Issue 4, pp.595-603

Belge Tipi:

Makale

Yayın Tarihi:

12/2019

Belge Dili:

Türkçe

Document ID (Org No.):

2000651044

Sıklık:

Üç Aylık

eISSN:

1308-7584

pISSN:

1308-7576