Exogenous application of pipecolic acid induces stomatal closure in Arabidopsis thaliana L.

Sercan Pazarlar

Makale detayları

Exogenous application of pipecolic acid induces stomatal closure in Arabidopsis thaliana L.

Yazarlar:

Sercan Pazarlar

Yayıncı:

Ege University

Ana Başlıklar:

Fitopatoloji

Dergi:

Ege Üniversitesi Ziraat Fakültesi Dergisi Vol.61 Issue 2, pp.143-150

Belge Tipi:

Makale

Yayın Tarihi:

1/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001322036

pISSN:

1018-8851

Anahtar kelimeler:

Özet:

 Objective: The major objectives of this study were i to determine whether exogenous Pipecolic acid treatment triggers the stomatal closure; ii to assess how the stomatal response is influenced by the method and concentrations of Pipecolic acid treatment; iii to investigate the response of Pipecolic acid-primed plants to the foliar bacterial pathogen Pseudomonas syringae pv. tomato DC3000 that invades plants through stomata. Material and Methods: Freshly harvested Arabidopsis leaves were immersed in MES-KCl buffer supplemented with 1 mM of D,L-Pipecolic acid for 2 h. Stomatal aperture was measured in epidermal strips collected from the abaxial side of the leaves. Stomatal aperture in Pipecolic acid-treated plants was also directly quantified after Pseudomonas syringae pv. tomato DC3000 inoculation. Results: The treatment with D,L-Pipecolic acid resulted in increased stomatal closure in a concentration-dependent manner. Treatments with 0.1 mM and 1 mM of D,L-Pipecolic acid led to a reduction in stomatal aperture by 32.5 and 54.7, respectively. Leaves treated with either 1 mM of D,L-Pipecolic acid or L-Pipecolic acid demonstrated similar stomatal apertures corresponding to 2.67 and 2.49 μm, respectively. The stomatal apertures did not exhibit a significant difference between the treatments following the Pseudomonas syringae pv. tomato DC3000 infection. Pipecolic acid-mediated enhanced defense is independent of stomatal immunity. Conclusion: Exogenous Pipecolic acid triggers preinvasion stomatal closure in Arabidopsis. There is no difference between pipecolic acid application methods soil drenching or foliar spray in terms of affecting stoma closure.

 Objective: The major objectives of this study were i to determine whether exogenous Pipecolic acid treatment triggers the stomatal closure; ii to assess how the stomatal response is influenced by the method and concentrations of Pipecolic acid treatment; iii to investigate the response of Pipecolic acid-primed plants to the foliar bacterial pathogen Pseudomonas syringae pv. tomato DC3000 that invades plants through stomata. Material and Methods: Freshly harvested Arabidopsis leaves were immersed in MES-KCl buffer supplemented with 1 mM of D,L-Pipecolic acid for 2 h. Stomatal aperture was measured in epidermal strips collected from the abaxial side of the leaves. Stomatal aperture in Pipecolic acid-treated plants was also directly quantified after Pseudomonas syringae pv. tomato DC3000 inoculation. Results: The treatment with D,L-Pipecolic acid resulted in increased stomatal closure in a concentration-dependent manner. Treatments with 0.1 mM and 1 mM of D,L-Pipecolic acid led to a reduction in stomatal aperture by 32.5 and 54.7, respectively. Leaves treated with either 1 mM of D,L-Pipecolic acid or L-Pipecolic acid demonstrated similar stomatal apertures corresponding to 2.67 and 2.49 μm, respectively. The stomatal apertures did not exhibit a significant difference between the treatments following the Pseudomonas syringae pv. tomato DC3000 infection. Pipecolic acid-mediated enhanced defense is independent of stomatal immunity. Conclusion: Exogenous Pipecolic acid triggers preinvasion stomatal closure in Arabidopsis. There is no difference between pipecolic acid application methods soil drenching or foliar spray in terms of affecting stoma closure.

 Objective: The major objectives of this study were i to determine whether exogenous Pipecolic acid treatment triggers the stomatal closure; ii to assess how the stomatal response is influenced by the method and concentrations of Pipecolic acid treatment; iii to investigate the response of Pipecolic acid-primed plants to the foliar bacterial pathogen Pseudomonas syringae pv. tomato DC3000 that invades plants through stomata. Material and Methods: Freshly harvested Arabidopsis leaves were immersed in MES-KCl buffer supplemented with 1 mM of D,L-Pipecolic acid for 2 h. Stomatal aperture was measured in epidermal strips collected from the abaxial side of the leaves. Stomatal aperture in Pipecolic acid-treated plants was also directly quantified after Pseudomonas syringae pv. tomato DC3000 inoculation. Results: The treatment with D,L-Pipecolic acid resulted in increased stomatal closure in a concentration-dependent manner. Treatments with 0.1 mM and 1 mM of D,L-Pipecolic acid led to a reduction in stomatal aperture by 32.5 and 54.7, respectively. Leaves treated with either 1 mM of D,L-Pipecolic acid or L-Pipecolic acid demonstrated similar stomatal apertures corresponding to 2.67 and 2.49 μm, respectively. The stomatal apertures did not exhibit a significant difference between the treatments following the Pseudomonas syringae pv. tomato DC3000 infection. Pipecolic acid-mediated enhanced defense is independent of stomatal immunity. Conclusion: Exogenous Pipecolic acid triggers preinvasion stomatal closure in Arabidopsis. There is no difference between pipecolic acid application methods soil drenching or foliar spray in terms of affecting stoma closure.

 Amaç: Bu çalışmanın ana amaçları şu şekildedir: i Ekzojen Pipekolik asit uygulamasının stoma kapanmasını tetikleyip tetiklemediğini belirlemek; ii Stoma tepkisinin Pipekolik asit uygulama yöntemi ve konsantrasyonlarından nasıl etkilendiğini saptamak; iii Pipekolik asitle uyarılmış bitkilerin, stomalar yoluyla bitkiye giriş yapan bakteriyel patojen Pseudomonas syringae pv. tomato DC3000e karşı tepkisini araştırmak. Materyal ve Yöntem: Taze koparılmış Arabidopsis yaprakları, 2 saat boyunca 1 mM D,L-Pipekolik asit içeren MES-KCl çözeltisine daldırılmıştır. Stoma açıklığı, yaprakların üst yüzeyinden toplanan epidermal şeritlerde ölçülmüştür. Pipekolik asit uygulanmış Arabidopsis bitkilerindeki stoma açıklığı da Pseudomonas syringae pv. tomato DC3000 inokulasyonundan 0, 2 ve 4 saat sonra alınan epidermal şeritlerde ölçülmüştür. Araştırma Bulguları: D,L-Pipekolik asit uygulaması, konsantrasyona bağlı bir şekilde stoma kapanmasının artmasına neden olmuştur. 0.1 mM ve 1 mM D,L-Pipekolik asit ile yapılan uygulamalar stoma açıklığında sırasıyla 32.5 ve 54.7 oranında bir azalmaya yol açmıştır. 1 mM D,L-Pipekolik asit veya L-Pipekolik asit ile muamele edilen yapraklar, sırasıyla 2,67 ve 2,49 μmye karşılık gelen benzer stoma açıklıkları göstermiştir. Bakteriyel enfeksiyonun ardından stoma açıklıkları uygulamalar arasında önemli bir fark sergilememiştir. Pipekolik asit aracılı gelişmiş savunma, stoma bağışıklığından bağımsızdır. Sonuç: Eksojen Pipekolik asit, Arabidopsiste enfeksiyon öncesi stoma kapanmasını tetikler. Pipekolik asit uygulama metotları arasında toprağı ıslatma ya da yaprağa püskürtme stoma kapanmasını etkilemesi bakımından fark yoktur.

Makale detayları

Exogenous application of pipecolic acid induces stomatal closure in Arabidopsis thaliana L.

Yazarlar:

Sercan Pazarlar

Yayıncı:

Ege University

Ana Başlıklar:

Fitopatoloji

Dergi:

Ege Üniversitesi Ziraat Fakültesi Dergisi Vol.61 Issue 2, pp.143-150

Belge Tipi:

Makale

Yayın Tarihi:

1/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001322036

pISSN:

1018-8851

L-Pip

plant defense responses

Pseudomonas syringae pv. tomato DC3000

systemic acquired resistance

stomatal defenseL-Pip

bitki savunma cevapları

Pseudomonas syringae pv. tomato DC3000

sistemik kazanılmış dayanıklılık

stoma savunması

Sayı

Makale detayları

Yazarlar: Sercan Pazarlar

Yayıncı: Ege University

Ana Başlıklar: Fitopatoloji

Dergi: Ege Üniversitesi Ziraat Fakültesi Dergisi Vol.61 Issue 2, pp.143-150

Belge Tipi: Makale

Yayın Tarihi: 1/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001322036

pISSN: 1018-8851

Sayı

Yazarlar:

Sercan Pazarlar

Yayıncı:

Ege University

Ana Başlıklar:

Fitopatoloji

Dergi:

Ege Üniversitesi Ziraat Fakültesi Dergisi Vol.61 Issue 2, pp.143-150

Belge Tipi:

Makale

Yayın Tarihi:

1/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001322036

pISSN:

1018-8851