Effect of Salinity Stress on Plant Growth Parameters and Antioxidant Activity In Some Cucumber Cultivars (Cucumis sativus L.) Grown Under In Vitro Conditions

Melek Demirel; Yeter Çilesiz; Ecem Kara; Gökhan Baktemur

Makale detayları

Effect of Salinity Stress on Plant Growth Parameters and Antioxidant Activity In Some Cucumber Cultivars (Cucumis sativus L.) Grown Under In Vitro Conditions

Yazarlar:

Melek Demirel | Yeter Çilesiz | Ecem Kara | Gökhan Baktemur

Yayıncı:

Mevlüt AKÇURA

Ana Başlıklar:

Agricultural Marine Biotechnology

Dergi:

Turkish Journal of Agricultural and Natural Sciences Vol.12 Issue 2, pp.531-542

Belge Tipi:

Makale

Yayın Tarihi:

4/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001345270

pISSN:

2148-3647

Anahtar kelimeler:

Özet:

 In this study, effects of salinity NaCl at different concentrations 0, 50, 100, 150, 200, 250, 300 mM on the growth of cucumber plants were determined in in vitro conditions. The study was carried out in the plant tissue culture laboratory of Agricultural Sciences and Technology Faculty of Sivas Science and Technology University Sivas, Türkiye . Experiments were conducted with 3 different cucumber varieties. Mureshige and Skoog MS was used as the basic nutrient medium. During the study, some parameters were evaluated such as germination rate , salt tolerance index , stem fresh and dry weight g, root fresh and dry weight g, actual water content , stem and root lengths and visual scale values. Also, antioxidant capacity were determined by DPPH and ABTS. When the effect of different NaCl doses on the varieties was evaluated, the highest antioxidant capacity was obtained from HD medium 173.21 μmol TE g-1 dw in the DPPH analysis and from HC medium 251.06 μmol TE g-1 dw in the ABTS analysis. In the study, the highest germination rate was obtained in the variety 1 94.36 and in the HA medium 96.05. In the actual water content, the variety average was the highest in the variety 2 91.16 and the medium average was the highest in the HA 91.71. The highest stem length was determined in the variety 1 26.40 mm and in the HA medium 24.48 mm. Considering the results of the study, although there were differences among the varieties, the increase in NaCl concentration negatively affected germination and plant growth in cucumber.

 In this study, effects of salinity NaCl at different concentrations 0, 50, 100, 150, 200, 250, 300 mM on the growth of cucumber plants were determined in in vitro conditions. The study was carried out in the plant tissue culture laboratory of Agricultural Sciences and Technology Faculty of Sivas Science and Technology University Sivas, Türkiye . Experiments were conducted with 3 different cucumber varieties. Mureshige and Skoog MS was used as the basic nutrient medium. During the study, some parameters were evaluated such as germination rate , salt tolerance index , stem fresh and dry weight g, root fresh and dry weight g, actual water content , stem and root lengths and visual scale values. Also, antioxidant capacity were determined by DPPH and ABTS. When the effect of different NaCl doses on the varieties was evaluated, the highest antioxidant capacity was obtained from HD medium 173.21 μmol TE g-1 dw in the DPPH analysis and from HC medium 251.06 μmol TE g-1 dw in the ABTS analysis. In the study, the highest germination rate was obtained in the variety 1 94.36 and in the HA medium 96.05. In the actual water content, the variety average was the highest in the variety 2 91.16 and the medium average was the highest in the HA 91.71. The highest stem length was determined in the variety 1 26.40 mm and in the HA medium 24.48 mm. Considering the results of the study, although there were differences among the varieties, the increase in NaCl concentration negatively affected germination and plant growth in cucumber.

 In this study, effects of salinity NaCl at different concentrations 0, 50, 100, 150, 200, 250, 300 mM on the growth of cucumber plants were determined in in vitro conditions. The study was carried out in the plant tissue culture laboratory of Agricultural Sciences and Technology Faculty of Sivas Science and Technology University Sivas, Türkiye . Experiments were conducted with 3 different cucumber varieties. Mureshige and Skoog MS was used as the basic nutrient medium. During the study, some parameters were evaluated such as germination rate , salt tolerance index , stem fresh and dry weight g, root fresh and dry weight g, actual water content , stem and root lengths and visual scale values. Also, antioxidant capacity were determined by DPPH and ABTS. When the effect of different NaCl doses on the varieties was evaluated, the highest antioxidant capacity was obtained from HD medium 173.21 μmol TE g-1 dw in the DPPH analysis and from HC medium 251.06 μmol TE g-1 dw in the ABTS analysis. In the study, the highest germination rate was obtained in the variety 1 94.36 and in the HA medium 96.05. In the actual water content, the variety average was the highest in the variety 2 91.16 and the medium average was the highest in the HA 91.71. The highest stem length was determined in the variety 1 26.40 mm and in the HA medium 24.48 mm. Considering the results of the study, although there were differences among the varieties, the increase in NaCl concentration negatively affected germination and plant growth in cucumber.

 In this study, effects of salinity NaCl at different concentrations 0, 50, 100, 150, 200, 250, 300 mM on the growth of cucumber plants were determined in in vitro conditions. The study was carried out in the plant tissue culture laboratory of Agricultural Sciences and Technology Faculty of Sivas Science and Technology University Sivas, Türkiye . Experiments were conducted with 3 different cucumber varieties. Mureshige and Skoog MS was used as the basic nutrient medium. During the study, some parameters were evaluated such as germination rate , salt tolerance index , stem fresh and dry weight g, root fresh and dry weight g, actual water content , stem and root lengths and visual scale values. Also, antioxidant capacity were determined by DPPH and ABTS. When the effect of different NaCl doses on the varieties was evaluated, the highest antioxidant capacity was obtained from HD medium 173.21 μmol TE g-1 dw in the DPPH analysis and from HC medium 251.06 μmol TE g-1 dw in the ABTS analysis. In the study, the highest germination rate was obtained in the variety 1 94.36 and in the HA medium 96.05. In the actual water content, the variety average was the highest in the variety 2 91.16 and the medium average was the highest in the HA 91.71. The highest stem length was determined in the variety 1 26.40 mm and in the HA medium 24.48 mm. Considering the results of the study, although there were differences among the varieties, the increase in NaCl concentration negatively affected germination and plant growth in cucumber.

Makale detayları

Effect of Salinity Stress on Plant Growth Parameters and Antioxidant Activity In Some Cucumber Cultivars (Cucumis sativus L.) Grown Under In Vitro Conditions

Yazarlar:

Melek Demirel | Yeter Çilesiz | Ecem Kara | Gökhan Baktemur

Yayıncı:

Mevlüt AKÇURA

Ana Başlıklar:

Agricultural Marine Biotechnology

Dergi:

Turkish Journal of Agricultural and Natural Sciences Vol.12 Issue 2, pp.531-542

Belge Tipi:

Makale

Yayın Tarihi:

4/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001345270

pISSN:

2148-3647

In vitro

NaCl

Cucumber

Antioxidant

Sayı

Makale detayları

Effect of Salinity Stress on Plant Growth Parameters and Antioxidant Activity In Some Cucumber Cultivars (Cucumis sativus L.) Grown Under In Vitro Conditions

Yazarlar: Melek Demirel | Yeter Çilesiz | Ecem Kara | Gökhan Baktemur

Yayıncı: Mevlüt AKÇURA

Ana Başlıklar: Agricultural Marine Biotechnology

Dergi: Turkish Journal of Agricultural and Natural Sciences Vol.12 Issue 2, pp.531-542

Belge Tipi: Makale

Yayın Tarihi: 4/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001345270

pISSN: 2148-3647

In vitro

NaCl

Cucumber

Antioxidant

Sayı

Yazarlar:

Melek Demirel | Yeter Çilesiz | Ecem Kara | Gökhan Baktemur

Yayıncı:

Mevlüt AKÇURA

Ana Başlıklar:

Agricultural Marine Biotechnology

Dergi:

Turkish Journal of Agricultural and Natural Sciences Vol.12 Issue 2, pp.531-542

Belge Tipi:

Makale

Yayın Tarihi:

4/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001345270

pISSN:

2148-3647