Characterization of Rubidium-Based Nanoparticles by Green Synthesis and Their Effect on Colorectal Cancer Cells

Dilşad Özerkan; İshak Afşin Kariper

Makale detayları

Characterization of Rubidium-Based Nanoparticles by Green Synthesis and Their Effect on Colorectal Cancer Cells

Yazarlar:

Dilşad Özerkan | İshak Afşin Kariper

Yayıncı:

Istanbul University

Ana Başlıklar:

Clinical Sciences (Other)

Dergi:

Experimed Vol.13 Issue 2, pp.97-102

Belge Tipi:

Makale

Yayın Tarihi:

9/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001298773

pISSN:

2667-5846

Anahtar kelimeler:

Özet:

 Objective: Colorectal cancers pose a major threat along with increasing morbidity and mortality to human health worldwide. Therefore, it is crucial to develop effective and safe methods for tumor therapy. In recent years, nanoparticles have emerged as successful candidates for drug delivery into tumor tissue. The particle size of nanoparticles NPs is of great importance for passive tumor targeting. Therefore, in this study, we aimed to synthesize and characterize rubidium-based nanoparticles RbNPs from the moss Abietinella abietina AA and determine their anticancer effects on colorectal carcinoma cell line HCT116. Materials and Methods: A field emission scanning electron microscope FESEM, dynamic light scattering DLS, energy dispersive X-ray analysis EDX, UVVIS and fourier transform infrared FTIR spectrophotometers were used to characterize the RbNPs. To study the cytotoxicity, a sulforhodamine B SRB assay was performed in colorectal carcinoma cell cultures. Results: As a result, RbNPs- AA developed with an average particle size of about 70 nm. RbNPs- AA proved to be cytotoxic at lower doses than free AA, as it decreased cell viability at half the amount of free AA 14.25 µgmL. Conclusion: The availability of RbNPs, particularly for the treatment of colorectal cancer, is evidenced by the fact that all the data collected are highly relevant.

 Objective: Colorectal cancers pose a major threat along with increasing morbidity and mortality to human health worldwide. Therefore, it is crucial to develop effective and safe methods for tumor therapy. In recent years, nanoparticles have emerged as successful candidates for drug delivery into tumor tissue. The particle size of nanoparticles NPs is of great importance for passive tumor targeting. Therefore, in this study, we aimed to synthesize and characterize rubidium-based nanoparticles RbNPs from the moss Abietinella abietina AA and determine their anticancer effects on colorectal carcinoma cell line HCT116. Materials and Methods: A field emission scanning electron microscope FESEM, dynamic light scattering DLS, energy dispersive X-ray analysis EDX, UVVIS and fourier transform infrared FTIR spectrophotometers were used to characterize the RbNPs. To study the cytotoxicity, a sulforhodamine B SRB assay was performed in colorectal carcinoma cell cultures. Results: As a result, RbNPs- AA developed with an average particle size of about 70 nm. RbNPs- AA proved to be cytotoxic at lower doses than free AA, as it decreased cell viability at half the amount of free AA 14.25 µgmL. Conclusion: The availability of RbNPs, particularly for the treatment of colorectal cancer, is evidenced by the fact that all the data collected are highly relevant.

 Objective: Colorectal cancers pose a major threat along with increasing morbidity and mortality to human health worldwide. Therefore, it is crucial to develop effective and safe methods for tumor therapy. In recent years, nanoparticles have emerged as successful candidates for drug delivery into tumor tissue. The particle size of nanoparticles NPs is of great importance for passive tumor targeting. Therefore, in this study, we aimed to synthesize and characterize rubidium-based nanoparticles RbNPs from the moss Abietinella abietina AA and determine their anticancer effects on colorectal carcinoma cell line HCT116. Materials and Methods: A field emission scanning electron microscope FESEM, dynamic light scattering DLS, energy dispersive X-ray analysis EDX, UVVIS and fourier transform infrared FTIR spectrophotometers were used to characterize the RbNPs. To study the cytotoxicity, a sulforhodamine B SRB assay was performed in colorectal carcinoma cell cultures. Results: As a result, RbNPs- AA developed with an average particle size of about 70 nm. RbNPs- AA proved to be cytotoxic at lower doses than free AA, as it decreased cell viability at half the amount of free AA 14.25 µgmL. Conclusion: The availability of RbNPs, particularly for the treatment of colorectal cancer, is evidenced by the fact that all the data collected are highly relevant.

 Objective: Colorectal cancers pose a major threat along with increasing morbidity and mortality to human health worldwide. Therefore, it is crucial to develop effective and safe methods for tumor therapy. In recent years, nanoparticles have emerged as successful candidates for drug delivery into tumor tissue. The particle size of nanoparticles NPs is of great importance for passive tumor targeting. Therefore, in this study, we aimed to synthesize and characterize rubidium-based nanoparticles RbNPs from the moss Abietinella abietina AA and determine their anticancer effects on colorectal carcinoma cell line HCT116. Materials and Methods: A field emission scanning electron microscope FESEM, dynamic light scattering DLS, energy dispersive X-ray analysis EDX, UVVIS and fourier transform infrared FTIR spectrophotometers were used to characterize the RbNPs. To study the cytotoxicity, a sulforhodamine B SRB assay was performed in colorectal carcinoma cell cultures. Results: As a result, RbNPs- AA developed with an average particle size of about 70 nm. RbNPs- AA proved to be cytotoxic at lower doses than free AA, as it decreased cell viability at half the amount of free AA 14.25 µgmL. Conclusion: The availability of RbNPs, particularly for the treatment of colorectal cancer, is evidenced by the fact that all the data collected are highly relevant.

Makale detayları

Characterization of Rubidium-Based Nanoparticles by Green Synthesis and Their Effect on Colorectal Cancer Cells

Yazarlar:

Dilşad Özerkan | İshak Afşin Kariper

Yayıncı:

Istanbul University

Ana Başlıklar:

Clinical Sciences (Other)

Dergi:

Experimed Vol.13 Issue 2, pp.97-102

Belge Tipi:

Makale

Yayın Tarihi:

9/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001298773

pISSN:

2667-5846

Colorectal cancer

rubidium nanoparticles

green synthesis

Sayı

Makale detayları

Characterization of Rubidium-Based Nanoparticles by Green Synthesis and Their Effect on Colorectal Cancer Cells

Yazarlar: Dilşad Özerkan | İshak Afşin Kariper

Yayıncı: Istanbul University

Ana Başlıklar: Clinical Sciences (Other)

Dergi: Experimed Vol.13 Issue 2, pp.97-102

Belge Tipi: Makale

Yayın Tarihi: 9/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001298773

pISSN: 2667-5846

Colorectal cancer

rubidium nanoparticles

green synthesis

Sayı

Yazarlar:

Dilşad Özerkan | İshak Afşin Kariper

Yayıncı:

Istanbul University

Ana Başlıklar:

Clinical Sciences (Other)

Dergi:

Experimed Vol.13 Issue 2, pp.97-102

Belge Tipi:

Makale

Yayın Tarihi:

9/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001298773

pISSN:

2667-5846