Anti-proliferative impact of three Schiff base platinum (II) complexes against human breast cancer cell line

Samaneh Zolghadri

Makale detayları

Anti-proliferative impact of three Schiff base platinum (II) complexes against human breast cancer cell line

Yazarlar:

Samaneh Zolghadri

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmaceutical Chemistry

Dergi:

Journal of Research in Pharmacy Vol.26 Issue 6, pp.1665-1675

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001347776

pISSN:

2630-6344

Anahtar kelimeler:

Özet:

 Treatment approaches for breast cancer BC are challenging in terms of tumor recurrence and drug resistance. We investigated the antitumor activity of three platinum II complexes bearing Schiff base ligands against breast cancer cells MCF-7. MCF-7 cells were treated with three platinum complexes and drug cytotoxicity was evaluated using an MTT assay. To quantify apoptosis and to confirm the mode of cell death, flowcytometry analysis using annexin V-FITC-PI kit and DNA fragmentation assay was used, respectively. The possible cytotoxic mechanism of action of these platinum compounds was also determined through mRNA expression analysis by the Real time PCR method. All complexes show a dose-dependent inhibitory effect on cell viability. flowcytometry analysis shows that the average percentage of apoptosis in 50 µM concentration of all complexes is 233.5 and DNA fragmentation assay confirmed apoptosis. Treatment of cells with complexes significantly decreases mRNA expression of topoisomerase 1 and 2 and Bcl-2 as compared to untreated control cells. Also, our results show that both caspase 6 and 9 genes expression levels were induced at high dose 50 µM of complexes. Our findings indicate that three Schiff base platinum II complexes have potent antiproliferative effect against breast cancer cells and induce apoptosis via the mitochondrial pathway. Also, our data provide evidence for a possible role of Schiff base platinum complexes in inhibition of breast tumor progression through downregulation of HIF-1α, topoisomerase 1 and 2 genes. These data support the idea that these complexes would be promising anti-breast cancer drugs.

 Treatment approaches for breast cancer BC are challenging in terms of tumor recurrence and drug resistance. We investigated the antitumor activity of three platinum II complexes bearing Schiff base ligands against breast cancer cells MCF-7. MCF-7 cells were treated with three platinum complexes and drug cytotoxicity was evaluated using an MTT assay. To quantify apoptosis and to confirm the mode of cell death, flowcytometry analysis using annexin V-FITC-PI kit and DNA fragmentation assay was used, respectively. The possible cytotoxic mechanism of action of these platinum compounds was also determined through mRNA expression analysis by the Real time PCR method. All complexes show a dose-dependent inhibitory effect on cell viability. flowcytometry analysis shows that the average percentage of apoptosis in 50 µM concentration of all complexes is 233.5 and DNA fragmentation assay confirmed apoptosis. Treatment of cells with complexes significantly decreases mRNA expression of topoisomerase 1 and 2 and Bcl-2 as compared to untreated control cells. Also, our results show that both caspase 6 and 9 genes expression levels were induced at high dose 50 µM of complexes. Our findings indicate that three Schiff base platinum II complexes have potent antiproliferative effect against breast cancer cells and induce apoptosis via the mitochondrial pathway. Also, our data provide evidence for a possible role of Schiff base platinum complexes in inhibition of breast tumor progression through downregulation of HIF-1α, topoisomerase 1 and 2 genes. These data support the idea that these complexes would be promising anti-breast cancer drugs.

 Treatment approaches for breast cancer BC are challenging in terms of tumor recurrence and drug resistance. We investigated the antitumor activity of three platinum II complexes bearing Schiff base ligands against breast cancer cells MCF-7. MCF-7 cells were treated with three platinum complexes and drug cytotoxicity was evaluated using an MTT assay. To quantify apoptosis and to confirm the mode of cell death, flowcytometry analysis using annexin V-FITC-PI kit and DNA fragmentation assay was used, respectively. The possible cytotoxic mechanism of action of these platinum compounds was also determined through mRNA expression analysis by the Real time PCR method. All complexes show a dose-dependent inhibitory effect on cell viability. flowcytometry analysis shows that the average percentage of apoptosis in 50 µM concentration of all complexes is 233.5 and DNA fragmentation assay confirmed apoptosis. Treatment of cells with complexes significantly decreases mRNA expression of topoisomerase 1 and 2 and Bcl-2 as compared to untreated control cells. Also, our results show that both caspase 6 and 9 genes expression levels were induced at high dose 50 µM of complexes. Our findings indicate that three Schiff base platinum II complexes have potent antiproliferative effect against breast cancer cells and induce apoptosis via the mitochondrial pathway. Also, our data provide evidence for a possible role of Schiff base platinum complexes in inhibition of breast tumor progression through downregulation of HIF-1α, topoisomerase 1 and 2 genes. These data support the idea that these complexes would be promising anti-breast cancer drugs.

 Treatment approaches for breast cancer BC are challenging in terms of tumor recurrence and drug resistance. We investigated the antitumor activity of three platinum II complexes bearing Schiff base ligands against breast cancer cells MCF-7. MCF-7 cells were treated with three platinum complexes and drug cytotoxicity was evaluated using an MTT assay. To quantify apoptosis and to confirm the mode of cell death, flowcytometry analysis using annexin V-FITC-PI kit and DNA fragmentation assay was used, respectively. The possible cytotoxic mechanism of action of these platinum compounds was also determined through mRNA expression analysis by the Real time PCR method. All complexes show a dose-dependent inhibitory effect on cell viability. flowcytometry analysis shows that the average percentage of apoptosis in 50 µM concentration of all complexes is 233.5 and DNA fragmentation assay confirmed apoptosis. Treatment of cells with complexes significantly decreases mRNA expression of topoisomerase 1 and 2 and Bcl-2 as compared to untreated control cells. Also, our results show that both caspase 6 and 9 genes expression levels were induced at high dose 50 µM of complexes. Our findings indicate that three Schiff base platinum II complexes have potent antiproliferative effect against breast cancer cells and induce apoptosis via the mitochondrial pathway. Also, our data provide evidence for a possible role of Schiff base platinum complexes in inhibition of breast tumor progression through downregulation of HIF-1α, topoisomerase 1 and 2 genes. These data support the idea that these complexes would be promising anti-breast cancer drugs.

Makale detayları

Anti-proliferative impact of three Schiff base platinum (II) complexes against human breast cancer cell line

Yazarlar:

Samaneh Zolghadri

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmaceutical Chemistry

Dergi:

Journal of Research in Pharmacy Vol.26 Issue 6, pp.1665-1675

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001347776

pISSN:

2630-6344

Platinum (II) complexes

schiff base ligands

apoptosis

MCF-7 cell line

Sayı

Makale detayları

Anti-proliferative impact of three Schiff base platinum (II) complexes against human breast cancer cell line

Yazarlar: Samaneh Zolghadri

Yayıncı: Marmara University

Ana Başlıklar: Pharmaceutical Chemistry

Dergi: Journal of Research in Pharmacy Vol.26 Issue 6, pp.1665-1675

Belge Tipi: Makale

Yayın Tarihi: 6/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001347776

pISSN: 2630-6344

Platinum (II) complexes

schiff base ligands

apoptosis

MCF-7 cell line

Sayı

Yazarlar:

Samaneh Zolghadri

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmaceutical Chemistry

Dergi:

Journal of Research in Pharmacy Vol.26 Issue 6, pp.1665-1675

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001347776

pISSN:

2630-6344