Iridoids and flavonoids from the aerial parts of Gentiana asclepiadea L. with anti-inflammatory and analgesic activities

Rima Konya Konuk; Rengin Reis; Hande Sipahi; Hasan Kırmızıbekmez

Makale detayları

Iridoids and flavonoids from the aerial parts of Gentiana asclepiadea L. with anti-inflammatory and analgesic activities

Yazarlar:

Rima Konya Konuk | Rengin Reis | Hande Sipahi | Hasan Kırmızıbekmez

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmacognosy

Dergi:

Journal of Research in Pharmacy Vol.27 Issue 3, pp.1202-1212

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001347895

pISSN:

2630-6344

Anahtar kelimeler:

Özet:

 Using various chromatographic methods, seven compounds, including two secoiridoid glycosides; depressine 1 and gentiopicroside 2, one iridoid glycoside; loganic acid 3, two flavone-C-glycosides; isoorientin 4 and isovitexin 5, one xanthone-C-glycoside, mangiferin 6 as well as a nucleoside; adenosine 7 were isolated from the MeOH extract prepared from the aerial parts of Gentiana asclepiadea L. Their structures were elucidated unambiguously by spectroscopic methods such as 1D and 2D NMR as well as HRESIMS. The anti-inflammatory and analgesic activities of the isolated compounds were also evaluated in vitro. Among the tested compounds, 1, 2, and 4 showed potent anti-inflammatory activity through both nitrite and IL-6 pathways at 200 µM. Besides, compound 1 exhibited the highest decrease in PGE2 level, with a higher inhibition rate compared to positive control indomethacin.

 Using various chromatographic methods, seven compounds, including two secoiridoid glycosides; depressine 1 and gentiopicroside 2, one iridoid glycoside; loganic acid 3, two flavone-C-glycosides; isoorientin 4 and isovitexin 5, one xanthone-C-glycoside, mangiferin 6 as well as a nucleoside; adenosine 7 were isolated from the MeOH extract prepared from the aerial parts of Gentiana asclepiadea L. Their structures were elucidated unambiguously by spectroscopic methods such as 1D and 2D NMR as well as HRESIMS. The anti-inflammatory and analgesic activities of the isolated compounds were also evaluated in vitro. Among the tested compounds, 1, 2, and 4 showed potent anti-inflammatory activity through both nitrite and IL-6 pathways at 200 µM. Besides, compound 1 exhibited the highest decrease in PGE2 level, with a higher inhibition rate compared to positive control indomethacin.

 Using various chromatographic methods, seven compounds, including two secoiridoid glycosides; depressine 1 and gentiopicroside 2, one iridoid glycoside; loganic acid 3, two flavone-C-glycosides; isoorientin 4 and isovitexin 5, one xanthone-C-glycoside, mangiferin 6 as well as a nucleoside; adenosine 7 were isolated from the MeOH extract prepared from the aerial parts of Gentiana asclepiadea L. Their structures were elucidated unambiguously by spectroscopic methods such as 1D and 2D NMR as well as HRESIMS. The anti-inflammatory and analgesic activities of the isolated compounds were also evaluated in vitro. Among the tested compounds, 1, 2, and 4 showed potent anti-inflammatory activity through both nitrite and IL-6 pathways at 200 µM. Besides, compound 1 exhibited the highest decrease in PGE2 level, with a higher inhibition rate compared to positive control indomethacin.

 Using various chromatographic methods, seven compounds, including two secoiridoid glycosides; depressine 1 and gentiopicroside 2, one iridoid glycoside; loganic acid 3, two flavone-C-glycosides; isoorientin 4 and isovitexin 5, one xanthone-C-glycoside, mangiferin 6 as well as a nucleoside; adenosine 7 were isolated from the MeOH extract prepared from the aerial parts of Gentiana asclepiadea L. Their structures were elucidated unambiguously by spectroscopic methods such as 1D and 2D NMR as well as HRESIMS. The anti-inflammatory and analgesic activities of the isolated compounds were also evaluated in vitro. Among the tested compounds, 1, 2, and 4 showed potent anti-inflammatory activity through both nitrite and IL-6 pathways at 200 µM. Besides, compound 1 exhibited the highest decrease in PGE2 level, with a higher inhibition rate compared to positive control indomethacin.

Makale detayları

Iridoids and flavonoids from the aerial parts of Gentiana asclepiadea L. with anti-inflammatory and analgesic activities

Yazarlar:

Rima Konya Konuk | Rengin Reis | Hande Sipahi | Hasan Kırmızıbekmez

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmacognosy

Dergi:

Journal of Research in Pharmacy Vol.27 Issue 3, pp.1202-1212

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001347895

pISSN:

2630-6344

Gentiana asclepiadea

Gentianaceae

secoiridoid

depressine

anti-inflammatory and analgesic activity

Sayı

Makale detayları

Iridoids and flavonoids from the aerial parts of Gentiana asclepiadea L. with anti-inflammatory and analgesic activities

Yazarlar: Rima Konya Konuk | Rengin Reis | Hande Sipahi | Hasan Kırmızıbekmez

Yayıncı: Marmara University

Ana Başlıklar: Pharmacognosy

Dergi: Journal of Research in Pharmacy Vol.27 Issue 3, pp.1202-1212

Belge Tipi: Makale

Yayın Tarihi: 6/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001347895

pISSN: 2630-6344

Gentiana asclepiadea

Gentianaceae

secoiridoid

depressine

anti-inflammatory and analgesic activity

Sayı

Yazarlar:

Rima Konya Konuk | Rengin Reis | Hande Sipahi | Hasan Kırmızıbekmez

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmacognosy

Dergi:

Journal of Research in Pharmacy Vol.27 Issue 3, pp.1202-1212

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001347895

pISSN:

2630-6344