Metformin Alleviates Doxorubicin-induced Liver Injury in Rats via Reducing Oxidative Stress, inflammation and Excessive Cell Death

Cevat Gençer; Alpaslan Gökçimen; Erhan Bayraktar; Fatih Birtekocak

Makale detayları

Metformin Alleviates Doxorubicin-induced Liver Injury in Rats via Reducing Oxidative Stress, inflammation and Excessive Cell Death

Yazarlar:

Cevat Gençer | Alpaslan Gökçimen | Erhan Bayraktar | Fatih Birtekocak

Yayıncı:

Aydin Adnan Menderes University

Ana Başlıklar:

Biochemistry and Cell Biology (Other)

Dergi:

Meandros Medical And Dental Journal Vol.24 Issue 2, pp.148-154

Belge Tipi:

Makale

Yayın Tarihi:

6/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001348388

pISSN:

2149-9063

Anahtar kelimeler:

Özet:

 Objective: The liver is one of the most important internal organs in the human body and has high regenerative properties. It performs the functions of protein synthesis, intake, storage, and distribution of nutrients and vitamins from the blood. In this study, we investigated the curative effect of metformin Met on doxorubicin DOX-induced liver damage. Materials and Methods: A total of 32 Wistar-albino rats were divided into four groups: control, Met, DOX, and DOX  Met groups. The DOX and DOX  Met groups received four doses of DOX. Met was gavaged daily for 15 days in the DOX  Met and Met groups. Structural liver injury was evaluated with hematoxylin-eosin, picro-sirius, TUNEL, and nuclear factor kB NF-kB antibody staining. Alanine aminotransferase ALT, aspartate aminotransferase AST, total antioxidative status TAS, total oxidative status TOS, and hydroxyproline levels were measured as biochemical parameters. Results: The DOX group was found to have a significant structural liver injury characterized by hyperchromatic nuclei in hepatocytes, widespread sinusoidal dilatations, and granular and vacuolar degeneration. Increased NF-kB staining and the apoptotic index were also detected in the DOX group. Biochemical tests revealed an increase in ALT, AST, and TOS levels and a decrease in TAS levels in the DOX group. Met administration provided a significant improvement in the structural changes caused by DOX. In addition, the DOX  Met group had lower NF-kB staining, apoptotic index, ALT, and TOS levels and a higher TAS level compared to the DOX group. Conclusion: Our findings indicate that Met alleviates DOX-induced structural liver injury by reducing oxidative stress, inflammation, and excessive cell death.

 Objective: The liver is one of the most important internal organs in the human body and has high regenerative properties. It performs the functions of protein synthesis, intake, storage, and distribution of nutrients and vitamins from the blood. In this study, we investigated the curative effect of metformin Met on doxorubicin DOX-induced liver damage. Materials and Methods: A total of 32 Wistar-albino rats were divided into four groups: control, Met, DOX, and DOX  Met groups. The DOX and DOX  Met groups received four doses of DOX. Met was gavaged daily for 15 days in the DOX  Met and Met groups. Structural liver injury was evaluated with hematoxylin-eosin, picro-sirius, TUNEL, and nuclear factor kB NF-kB antibody staining. Alanine aminotransferase ALT, aspartate aminotransferase AST, total antioxidative status TAS, total oxidative status TOS, and hydroxyproline levels were measured as biochemical parameters. Results: The DOX group was found to have a significant structural liver injury characterized by hyperchromatic nuclei in hepatocytes, widespread sinusoidal dilatations, and granular and vacuolar degeneration. Increased NF-kB staining and the apoptotic index were also detected in the DOX group. Biochemical tests revealed an increase in ALT, AST, and TOS levels and a decrease in TAS levels in the DOX group. Met administration provided a significant improvement in the structural changes caused by DOX. In addition, the DOX  Met group had lower NF-kB staining, apoptotic index, ALT, and TOS levels and a higher TAS level compared to the DOX group. Conclusion: Our findings indicate that Met alleviates DOX-induced structural liver injury by reducing oxidative stress, inflammation, and excessive cell death.

 Objective: The liver is one of the most important internal organs in the human body and has high regenerative properties. It performs the functions of protein synthesis, intake, storage, and distribution of nutrients and vitamins from the blood. In this study, we investigated the curative effect of metformin Met on doxorubicin DOX-induced liver damage. Materials and Methods: A total of 32 Wistar-albino rats were divided into four groups: control, Met, DOX, and DOX  Met groups. The DOX and DOX  Met groups received four doses of DOX. Met was gavaged daily for 15 days in the DOX  Met and Met groups. Structural liver injury was evaluated with hematoxylin-eosin, picro-sirius, TUNEL, and nuclear factor kB NF-kB antibody staining. Alanine aminotransferase ALT, aspartate aminotransferase AST, total antioxidative status TAS, total oxidative status TOS, and hydroxyproline levels were measured as biochemical parameters. Results: The DOX group was found to have a significant structural liver injury characterized by hyperchromatic nuclei in hepatocytes, widespread sinusoidal dilatations, and granular and vacuolar degeneration. Increased NF-kB staining and the apoptotic index were also detected in the DOX group. Biochemical tests revealed an increase in ALT, AST, and TOS levels and a decrease in TAS levels in the DOX group. Met administration provided a significant improvement in the structural changes caused by DOX. In addition, the DOX  Met group had lower NF-kB staining, apoptotic index, ALT, and TOS levels and a higher TAS level compared to the DOX group. Conclusion: Our findings indicate that Met alleviates DOX-induced structural liver injury by reducing oxidative stress, inflammation, and excessive cell death.

 Objective: The liver is one of the most important internal organs in the human body and has high regenerative properties. It performs the functions of protein synthesis, intake, storage, and distribution of nutrients and vitamins from the blood. In this study, we investigated the curative effect of metformin Met on doxorubicin DOX-induced liver damage. Materials and Methods: A total of 32 Wistar-albino rats were divided into four groups: control, Met, DOX, and DOX  Met groups. The DOX and DOX  Met groups received four doses of DOX. Met was gavaged daily for 15 days in the DOX  Met and Met groups. Structural liver injury was evaluated with hematoxylin-eosin, picro-sirius, TUNEL, and nuclear factor kB NF-kB antibody staining. Alanine aminotransferase ALT, aspartate aminotransferase AST, total antioxidative status TAS, total oxidative status TOS, and hydroxyproline levels were measured as biochemical parameters. Results: The DOX group was found to have a significant structural liver injury characterized by hyperchromatic nuclei in hepatocytes, widespread sinusoidal dilatations, and granular and vacuolar degeneration. Increased NF-kB staining and the apoptotic index were also detected in the DOX group. Biochemical tests revealed an increase in ALT, AST, and TOS levels and a decrease in TAS levels in the DOX group. Met administration provided a significant improvement in the structural changes caused by DOX. In addition, the DOX  Met group had lower NF-kB staining, apoptotic index, ALT, and TOS levels and a higher TAS level compared to the DOX group. Conclusion: Our findings indicate that Met alleviates DOX-induced structural liver injury by reducing oxidative stress, inflammation, and excessive cell death.

Makale detayları

Metformin Alleviates Doxorubicin-induced Liver Injury in Rats via Reducing Oxidative Stress, inflammation and Excessive Cell Death

Yazarlar:

Cevat Gençer | Alpaslan Gökçimen | Erhan Bayraktar | Fatih Birtekocak

Yayıncı:

Aydin Adnan Menderes University

Ana Başlıklar:

Biochemistry and Cell Biology (Other)

Dergi:

Meandros Medical And Dental Journal Vol.24 Issue 2, pp.148-154

Belge Tipi:

Makale

Yayın Tarihi:

6/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001348388

pISSN:

2149-9063

Doxorubicin

metformin

liver injury

apoptosis

oxidative stress

Sayı

Makale detayları

Metformin Alleviates Doxorubicin-induced Liver Injury in Rats via Reducing Oxidative Stress, inflammation and Excessive Cell Death

Yazarlar: Cevat Gençer | Alpaslan Gökçimen | Erhan Bayraktar | Fatih Birtekocak

Yayıncı: Aydin Adnan Menderes University

Ana Başlıklar: Biochemistry and Cell Biology (Other)

Dergi: Meandros Medical And Dental Journal Vol.24 Issue 2, pp.148-154

Belge Tipi: Makale

Yayın Tarihi: 6/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001348388

pISSN: 2149-9063

Doxorubicin

metformin

liver injury

apoptosis

oxidative stress

Sayı

Yazarlar:

Cevat Gençer | Alpaslan Gökçimen | Erhan Bayraktar | Fatih Birtekocak

Yayıncı:

Aydin Adnan Menderes University

Ana Başlıklar:

Biochemistry and Cell Biology (Other)

Dergi:

Meandros Medical And Dental Journal Vol.24 Issue 2, pp.148-154

Belge Tipi:

Makale

Yayın Tarihi:

6/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001348388

pISSN:

2149-9063