Study of Paraoxonase1 Enzyme for Women with Breast Cancer (Biochemical and Molecular Genetics Study)

Luma Abdalmunim BAKER; Hazha Jamal HIDAYET; Nahida S. AL-CHALABI

Makale detayları

Study of Paraoxonase1 Enzyme for Women with Breast Cancer (Biochemical and Molecular Genetics Study)

Yazarlar:

Luma Abdalmunim BAKER | Hazha Jamal HIDAYET | Nahida S. AL-CHALABI

Dergi:

The Eurasia Proceedings of Science Technology Engineering and Mathematics 2018 Issue 3, pp.56-71

Belge Tipi:

Makale

Yayın Tarihi:

11/2018

Belge Dili:

İngilizce

Document ID (Org No.):

2000661020

Özet:

 


The
research include studying biochemical and genetic molecular study for
paraoxonase1 enzyme and identify some biochemical parameters for serum blood
samples 68 women with breast cancer and follow-up after take them three doses
of chemotherapy and comparative with 50 normal female as control group.The
result showed for women with breast cancer a significant increase in (TC,MDA
,Total protein, U,A , calcium and BMI) when compared with normal women, the
results also showed for women who have taken chemical doses and women with
breast cancer significant decreased in (PON1, GSH) compared with normal women,
the results showed for women who have taken 
the first dose significant increase in (MDA,TP, Calcium, U.A , BMI),
while second dose and the third dose chemotherapy found significant increase in
(TP, U.A ,Calcium ) and significant decreased for (PON1). The results included
sequential analysis for PON1 enzyme ,it has shown results for genetic analyzed
selected exon (Exon4), where DNA extraction for normal women and breast cancer
women and taken their doses of chemotherapy and measure DNA concentration and
purity using Nano-Drop Spectrophotometric Analysis where the result show the
DNA concentration ranged between (5.2-68.4) ng/ml where concentration mean for
women with breast cancer (26.97) ng/ml and (17.49) ng/ml for normal women while
the first dose 24.74 ng/ml , (16.16-9.70)ng/ml for second and third dose
respectively . It has also been used PCR technique amplification  and test products PCR analysis of sequential
technique by using gel electrophoresis where packet appeared clear without
deformation and tested PCR product sequencing for exon4 by capillary automated
sequencer and  comparing the  result with the normal sequence of the oxon.,
the results shows for women have taken first dose deletion mutation called
(Remove) frame shift mutation clear nitrogen base one lead to change all codons
after deletion site so that all amino acid change in poly protien chain which
led to the loss of amino acid (Tyr.) . and mutation type of  silent mutation third mutation type of
substitution mutation (Replace amino acid (Thr.) by amino acid (Ser.).

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Makale detayları

Study of Paraoxonase1 Enzyme for Women with Breast Cancer (Biochemical and Molecular Genetics Study)

Yazarlar: Luma Abdalmunim BAKER | Hazha Jamal HIDAYET | Nahida S. AL-CHALABI

Dergi: The Eurasia Proceedings of Science Technology Engineering and Mathematics 2018 Issue 3, pp.56-71

Belge Tipi: Makale

Yayın Tarihi: 11/2018

Belge Dili: İngilizce

Document ID (Org No.): 2000661020

Sayı

Yazarlar:

Luma Abdalmunim BAKER | Hazha Jamal HIDAYET | Nahida S. AL-CHALABI

Dergi:

The Eurasia Proceedings of Science Technology Engineering and Mathematics 2018 Issue 3, pp.56-71

Belge Tipi:

Makale

Yayın Tarihi:

11/2018

Belge Dili:

İngilizce

Document ID (Org No.):

2000661020