Overexpression of the Glycine max chalcone isomerase (GmCHI) gene in transgenic  Talinum paniculatum plants

Thi Nhu Trang VU; Thi Hong Trang LE; Phu Hiep HOANG; Danh Thuong SY; Thi Thu Thuy VU; Hoang Mau CHU

Makale detayları

Overexpression of the Glycine max chalcone isomerase (GmCHI) gene in transgenic Talinum paniculatum plants

Yazarlar:

Thi Nhu Trang VU | Thi Hong Trang LE | Phu Hiep HOANG | Danh Thuong SY | Thi Thu Thuy VU | Hoang Mau CHU

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Botany 2018 Vol.42 Issue 5, pp.551-558

Belge Tipi:

Makale

Yayın Tarihi:

7/2018

Belge Dili:

Türkçe

Document ID (Org No.):

2000815010

Sıklık:

İki Aylık

eISSN:

1303-6106

pISSN:

1300-008X

Özet:

 

Talinum paniculatum plants contain flavonoids and saponins, which have strong antioxidant properties and are used in  the treatment of numerous diseases, such as inflammation, allergies, and gastric ulcers. Currently, there is no published research on  the flavonoid content of T. paniculatum plants; however, it has been determined that the species in the genus Talinum have very low  flavonoid content, and therefore the effective approach to enhance the flavonoid content in T. paniculatum plants is overexpression  of the Glycine max chalcone isomerase (GmCHI) gene. In this study, we analyzed GmCHI gene overexpression, which has increased  total flavonoid content in transgenic T. paniculatum lines. We have successfully transformed T. paniculatum plants with the pCB301-GmCHI vector and created 2 lines in generation T1 (T1-2.2 and T1-10) with a transgenic frequency of 0.27%. Recombinant CHI  protein expressed in transgenic lines has a molecular weight of approximately 25 kDa, and the concentration of this protein in the  two transgenic lines T1-2.2 and T1-10 was 4.29 ?g mg?1 and 6.14 ?g mg?1, respectively. Total flavonoid contents of the two transgenic  lines T1-2.2 and T1-10 were 4.24 mg g?1 and 2.74 mg g?1, respectively, which showed increases of 7.4-fold and 4.8-fold compared to  that of the nontransgenic plants, respectively. Thus, overexpression of the GmCHI gene enabled T. paniculatum plants to improve their  total flavonoid content. This work also confirmed the effectiveness of Agrobacterium-mediated plant transformation in enhancement of  flavonoid accumulation in T. paniculatum plants.

Makale detayları

Overexpression of the Glycine max chalcone isomerase (GmCHI) gene in transgenic Talinum paniculatum plants

Yazarlar: Thi Nhu Trang VU | Thi Hong Trang LE | Phu Hiep HOANG | Danh Thuong SY | Thi Thu Thuy VU | Hoang Mau CHU

Yayıncı: TUBITAK

Dergi: Turkish Journal of Botany 2018 Vol.42 Issue 5, pp.551-558

Belge Tipi: Makale

Yayın Tarihi: 7/2018

Belge Dili: Türkçe

Document ID (Org No.): 2000815010

Sıklık: İki Aylık

eISSN: 1303-6106

pISSN: 1300-008X

Sayı

Yazarlar:

Thi Nhu Trang VU | Thi Hong Trang LE | Phu Hiep HOANG | Danh Thuong SY | Thi Thu Thuy VU | Hoang Mau CHU

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Botany 2018 Vol.42 Issue 5, pp.551-558

Belge Tipi:

Makale

Yayın Tarihi:

7/2018

Belge Dili:

Türkçe

Document ID (Org No.):

2000815010

Sıklık:

İki Aylık

eISSN:

1303-6106

pISSN:

1300-008X