INVESTIGATION OF ANTIMICROBIAL, ANTIBIOFILM AND ANTI-QUORUM SENSING ACTIVITIES OF EXTRACTS FROM SCUTELLARIA YILDIRIMLII M. ÇİÇEK & A.E. YAPRAK

Damla Haddur Acikalin; Gülçin Saltan İşcan; Suna Sibel Rızvanoğlu; Hediye Kamuran İleri Özler; Mustafa Korkmazyiğit; Müjde Eryılmaz

Makale detayları

INVESTIGATION OF ANTIMICROBIAL, ANTIBIOFILM AND ANTI-QUORUM SENSING ACTIVITIES OF EXTRACTS FROM SCUTELLARIA YILDIRIMLII M. ÇİÇEK & A.E. YAPRAK

Yazarlar:

Damla Haddur Acikalin | Gülçin Saltan İşcan | Suna Sibel Rızvanoğlu | Hediye Kamuran İleri Özler | Mustafa Korkmazyiğit | Müjde Eryılmaz

Yayıncı:

Ankara University

Ana Başlıklar:

Farmakognozi | Farmasotik Mikrobiyoloji

Dergi:

Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.49 Issue 3, pp.756-765

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001356411

pISSN:

1015-3918

Anahtar kelimeler:

Özet:

 Objective: This study aims to investigate the antimicrobial, antibiofilm, and anti-quorum sensing anti-QS properties of extracts derived from the aerial parts of Scutellaria yildirimlii M. Çiçek  A.E. Yaprak. Material and Method: Using a liquid-liquid extraction method, petroleum ether PEE, ethyl acetate EAE, and aqueous AE sub-extracts were obtained from the total extract TE prepared with 80 methanol. Broth microdilution method assessed antimicrobial activity, microplate-based biofilm model and crystal violet assay assessed antibiofilm activity, and disk diffusion method assessed anti-quorum sensing activity Result and Discussion: The extracts exhibited MIC values ranging from 625 to 10000 µgml against the tested bacteria. Among them, the TE and PEE extracts showed the strongest antibacterial activity against both Gram-positive and Gram-negative test bacteria. Notably, only the PEE extract demonstrated antifungal activity against Candida albicans ATCC 10231, with an MIC value of 625 µgml, while no antifungal activity was observed for the other extracts. However, the antimicrobial effects were generally weak compared to standard antimicrobials. At a concentration of 2500 µgml, the biofilm inhibition percentages for AE, TE, PEE, and EAE extracts were 76.79, 57.47, 54.81, and 51.09, respectively. An anti-QS activity test, conducted based on MIC values against Chromobacterium violaceum ATCC 12472, revealed no violacein inhibition zones, indicating a lack of anti-QS activity. The test results demonstrated that S. yildirimlii possesses greater antimicrobial properties than many other Scutellaria species. This indicates that its extracts could serve as promising candidates for developing treatments against the increasing threat of antibiotic-resistant bacteria. Nonetheless, additional research is essential to thoroughly investigate and confirm the plants potential as a medicinal resource.

 Objective: This study aims to investigate the antimicrobial, antibiofilm, and anti-quorum sensing anti-QS properties of extracts derived from the aerial parts of Scutellaria yildirimlii M. Çiçek  A.E. Yaprak. Material and Method: Using a liquid-liquid extraction method, petroleum ether PEE, ethyl acetate EAE, and aqueous AE sub-extracts were obtained from the total extract TE prepared with 80 methanol. Broth microdilution method assessed antimicrobial activity, microplate-based biofilm model and crystal violet assay assessed antibiofilm activity, and disk diffusion method assessed anti-quorum sensing activity Result and Discussion: The extracts exhibited MIC values ranging from 625 to 10000 µgml against the tested bacteria. Among them, the TE and PEE extracts showed the strongest antibacterial activity against both Gram-positive and Gram-negative test bacteria. Notably, only the PEE extract demonstrated antifungal activity against Candida albicans ATCC 10231, with an MIC value of 625 µgml, while no antifungal activity was observed for the other extracts. However, the antimicrobial effects were generally weak compared to standard antimicrobials. At a concentration of 2500 µgml, the biofilm inhibition percentages for AE, TE, PEE, and EAE extracts were 76.79, 57.47, 54.81, and 51.09, respectively. An anti-QS activity test, conducted based on MIC values against Chromobacterium violaceum ATCC 12472, revealed no violacein inhibition zones, indicating a lack of anti-QS activity. The test results demonstrated that S. yildirimlii possesses greater antimicrobial properties than many other Scutellaria species. This indicates that its extracts could serve as promising candidates for developing treatments against the increasing threat of antibiotic-resistant bacteria. Nonetheless, additional research is essential to thoroughly investigate and confirm the plants potential as a medicinal resource.

 Objective: This study aims to investigate the antimicrobial, antibiofilm, and anti-quorum sensing anti-QS properties of extracts derived from the aerial parts of Scutellaria yildirimlii M. Çiçek  A.E. Yaprak. Material and Method: Using a liquid-liquid extraction method, petroleum ether PEE, ethyl acetate EAE, and aqueous AE sub-extracts were obtained from the total extract TE prepared with 80 methanol. Broth microdilution method assessed antimicrobial activity, microplate-based biofilm model and crystal violet assay assessed antibiofilm activity, and disk diffusion method assessed anti-quorum sensing activity Result and Discussion: The extracts exhibited MIC values ranging from 625 to 10000 µgml against the tested bacteria. Among them, the TE and PEE extracts showed the strongest antibacterial activity against both Gram-positive and Gram-negative test bacteria. Notably, only the PEE extract demonstrated antifungal activity against Candida albicans ATCC 10231, with an MIC value of 625 µgml, while no antifungal activity was observed for the other extracts. However, the antimicrobial effects were generally weak compared to standard antimicrobials. At a concentration of 2500 µgml, the biofilm inhibition percentages for AE, TE, PEE, and EAE extracts were 76.79, 57.47, 54.81, and 51.09, respectively. An anti-QS activity test, conducted based on MIC values against Chromobacterium violaceum ATCC 12472, revealed no violacein inhibition zones, indicating a lack of anti-QS activity. The test results demonstrated that S. yildirimlii possesses greater antimicrobial properties than many other Scutellaria species. This indicates that its extracts could serve as promising candidates for developing treatments against the increasing threat of antibiotic-resistant bacteria. Nonetheless, additional research is essential to thoroughly investigate and confirm the plants potential as a medicinal resource.

 Amaç: Bu çalışma kapsamında, Scutellaria yıldırımlii bitkisinin toprak üstü kısımlarından elde edilen ekstrelerin antimikrobiyal, antibiyofilm, ve çoğunluğu algılama mekanizması aktivite potansiyelinin araştırılması amaçlanmıştır. Gereç ve Yöntem: Sıvı- sıvı ekraksiyon metodu ile, 80 metanollü total ektsreden, petrol eteri PEE, etil asetat EAE, ve sulu AE alt ekstreler elde edilmiştir. Antimikrobiyal aktivite testi, broth mikrodilüsyon metodu ile; antibiyofilm aktivite testi, mikroplaka-bazlı biyofilm modeli ve kristal viyole deneyi ile; çoğunluğu algılama mekanizması aktivite testi, disk difüzyon metodu ile yapılmıştır. Sonuç ve Tartışma: Ekstreler, test edilen bakterilere karşı 625 ile 10.000 µgml arasında değişen MIC değerleri sergilemiştir. Bunlar arasında, TE ve PEE ekstreleri, hem Gram-pozitif hem de Gram-negatif test bakterilerine karşı en güçlü antibakteriyel aktiviteyi göstermiştir. Özellikle, PEE ekstreleri yalnızca Candida albicans ATCC 10231e karşı antifungal aktivite göstermiş olup, MIC değeri 625 µgml olarak belirlenmiştir. Diğer ekstrelerde ise herhangi bir antifungal aktivite gözlemlenmemiştir. Ancak, genel olarak, antimikrobiyal etkiler standart antimikrobiyallerle kıyaslandığında zayıf kalmıştır. 2500 µgml konsantrasyonda, AE, TE, PEE ve EAE ekstrelerinin biyofilm inhibisyon yüzdeleri sırasıyla 76.79, 57.47, 54.81 ve 51.09 olarak ölçülmüştür. Chromobacterium violaceum ATCC 12472ye karşı MIC değerlerine dayalı olarak gerçekleştirilen çoğunluğu algılama mekanizması aktivite testi, herhangi bir violasein inhibisyon bölgesi göstermemiştir; bu da çoğunluğu algılama mekanizması aktivitesinin bulunmadığını ortaya koymuştur. Test sonuçları, S. yıldırımliinin, birçok Scutellaria türüne kıyasla daha güçlü antimikrobiyal özelliklere sahip olduğunu göstermiştir. Bu durum, bitki ekstrelerinin antibiyotiklere dirençli bakterilere karşı tedavi geliştirme açısından umut verici adaylar olabileceğini düşündürmektedir. Bununla birlikte, bitkinin tıbbi potansiyelinin tam olarak anlaşılması ve doğrulanması için daha fazla araştırma yapılması gerekmektedir.

Makale detayları

INVESTIGATION OF ANTIMICROBIAL, ANTIBIOFILM AND ANTI-QUORUM SENSING ACTIVITIES OF EXTRACTS FROM SCUTELLARIA YILDIRIMLII M. ÇİÇEK & A.E. YAPRAK

Yazarlar:

Damla Haddur Acikalin | Gülçin Saltan İşcan | Suna Sibel Rızvanoğlu | Hediye Kamuran İleri Özler | Mustafa Korkmazyiğit | Müjde Eryılmaz

Yayıncı:

Ankara University

Ana Başlıklar:

Farmakognozi | Farmasotik Mikrobiyoloji

Dergi:

Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.49 Issue 3, pp.756-765

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001356411

pISSN:

1015-3918

Antibiofilm activity

antimicrobial activity

anti-quorum sensing activity

extraction

Scutellaria yildirimliiAntibiyofilm aktivite

antimikrobiyal aktivite

çoğunluğu algılama mekanizması aktivitesi

ekstraksiyon

Scutellaria yıldırımlii

Sayı

Makale detayları

Yazarlar: Damla Haddur Acikalin | Gülçin Saltan İşcan | Suna Sibel Rızvanoğlu | Hediye Kamuran İleri Özler | Mustafa Korkmazyiğit | Müjde Eryılmaz

Yayıncı: Ankara University

Ana Başlıklar: Farmakognozi | Farmasotik Mikrobiyoloji

Dergi: Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.49 Issue 3, pp.756-765

Belge Tipi: Makale

Yayın Tarihi: 9/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001356411

pISSN: 1015-3918

Sayı

Yazarlar:

Damla Haddur Acikalin | Gülçin Saltan İşcan | Suna Sibel Rızvanoğlu | Hediye Kamuran İleri Özler | Mustafa Korkmazyiğit | Müjde Eryılmaz

Yayıncı:

Ankara University

Ana Başlıklar:

Farmakognozi | Farmasotik Mikrobiyoloji

Dergi:

Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.49 Issue 3, pp.756-765

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001356411

pISSN:

1015-3918