PCR detection of Mycobacterium genavense DNA in fecal samples of caged birds

Orkun BABACAN; Bülent BAŞ; Barış SAREYYÜPOĞLU

doi:https://doi.org/10.33988/auvfd.603515

Makale detayları

PCR detection of Mycobacterium genavense DNA in fecal samples of caged birds

Yazarlar:

Orkun BABACAN | Bülent BAŞ | Barış SAREYYÜPOĞLU

Ana Başlıklar:

Hayvancılık

Dergi:

Ankara Üniversitesi Veteriner Fakültesi Dergisi 2020 Vol.67 Issue 2, pp.201-204

Belge Tipi:

Makale

Yayın Tarihi:

3/2020

Belge Dili:

İngilizce

Document ID (Org No.):

2000849526

DOI:

https://doi.org/10.33988/auvfd.603515

Özet:

 

In this study, pathogenic mycobacteria were investigated in fecal samples of caged birds by PCR. A total of 47 feces samples collected from 4 different aviaries in Ankara. DNA extraction from fecal samples was performed with a commercial kit using spin column technology. PCR was performed with designed primers respectively amplifying 274 base pairs (bp), 128 bp, 102 bp and 219 bp nucleotide sequences of specific genes (16SrRNA, ISI245, IS901 and hypothetical 21kDa protein gene) of Mycobacterium spp., Mycobacterium avium complex (MAC), Mycobacterium avium subsp. Avium and Mycobacterium genavense, respectively. Five samples were positive and harbored the sequence for the Mycobacterium spp., of 4 of these 5 samples was identified as M. genavense by PCR. As a conclusion of this study, which is the first announcement of the detection of M. genavense DNA in fecal samples of caged birds in Turkey, PCR was seen to be a rapid, sensitive, and a reliable method in detection of avian mycobacteriosis.

Makale detayları

PCR detection of Mycobacterium genavense DNA in fecal samples of caged birds

Yazarlar: Orkun BABACAN | Bülent BAŞ | Barış SAREYYÜPOĞLU

Ana Başlıklar: Hayvancılık

Dergi: Ankara Üniversitesi Veteriner Fakültesi Dergisi 2020 Vol.67 Issue 2, pp.201-204

Belge Tipi: Makale

Yayın Tarihi: 3/2020

Belge Dili: İngilizce

Document ID (Org No.): 2000849526

DOI: https://doi.org/10.33988/auvfd.603515

Sayı

Yazarlar:

Orkun BABACAN | Bülent BAŞ | Barış SAREYYÜPOĞLU

Ana Başlıklar:

Hayvancılık

Dergi:

Ankara Üniversitesi Veteriner Fakültesi Dergisi 2020 Vol.67 Issue 2, pp.201-204

Belge Tipi:

Makale

Yayın Tarihi:

3/2020

Belge Dili:

İngilizce

Document ID (Org No.):

2000849526

DOI:

https://doi.org/10.33988/auvfd.603515