Effects of Apelin-13 on Human Prostate Cancer Lines [İnsan Prostat Kanseri Hücre Serilerinde Apelin-13’ün Etkileri]

Suat TEKİN; Suleyman SANDAL; Cemil COLAK

Makale detayları

Effects of Apelin-13 on Human Prostate Cancer Lines [İnsan Prostat Kanseri Hücre Serilerinde Apelin-13’ün Etkileri]

Yazarlar:

Suat TEKİN | Suleyman SANDAL | Cemil COLAK

Yayıncı:

Inonu University

Dergi:

Medicine Science 2014 Vol.3 Issue 3, pp.1427-1441

Belge Tipi:

Makale

Yayın Tarihi:

9/2014

Belge Dili:

Türkçe

Document ID (Org No.):

2000828458

Sıklık:

Üç Aylık

eISSN:

2147-0634

pISSN:

2147-0634

Anahtar kelimeler:

Özet:

 

Prostate cancer is one of the most prevalent type of cancer in men. The exact solution for the treatment of different types of cancers has not been fully elucideted. Apelin secreted from adipose tissue reveals its effects by binding to APJ receptor. Obesity develops together with increased adipose tissue, and the increase of obesity related-apelin secretion is known. Obesity is a risk factor in the development of cancer, and considering both apelin and its receptor widely localized in the tissues of testis and prostate and apelin may have significant effects on prostate cancer. In the present study, 0.1, 1 and 10 nM concentrations of apelin-13 and three different testosterone concentrations (1, 10 and 100 nM) were separately aplied to human prostate cancer cells with LNCaP (androgen receptor positive) and DU-145 (androgen receptor negative). Then 1, 10 and 100 nM concentrations of testosterone hormone and 10 nM dose of apelin-13 incubated for 24 h in the prostate cancer cells were administrated and were allowed to incubate for 24 h. Effects of apelin-13 and testosterone on prostate diphenyltetrazolium bromide (MTT) assay. At the end of the experiment, DU-145 did not affected cell viability while the treatment with apelin -13 and testosterone increased the viability of LNCaP cells (p<0.001). The proliferation effect was strongly occured when LNCaP cells were treated with together apelin-13 and testosterone (p<0.001) but, no effect was observed in DU-145 cells. In conclusion; apelin-13 had similar proliferative effects with testosterone on LNCaP cells, but had no effect on DU-145 cells. These results suggest that the effects of apelin-13 are similar to testosterone, and it realises their effects through an androgen receptor-dependent mechanism.

 


Abstract

Prostate cancer is one of the most prevalent type of cancer in men. The exact solution for the treatment of different types of cancers has not been fully elucideted. Apelin secreted from adipose tissue reveals its effects by binding to APJ receptor. Obesity develops together with increased adipose tissue, and the increase of obesity related-apelin secretion is known. Obesity is a risk factor in the development of cancer, and considering both apelin and its receptor widely localized in the tissues of testis and prostate and apelin may have significant effects on prostate cancer. In the present study, 0.1, 1 and 10 nM concentrations of apelin-13 and three different testosterone concentrations (1, 10 and 100 nM) were separately aplied to human prostate cancer cells with LNCaP (androgen receptor positive) and DU-145 (androgen receptor negative). Then 1, 10 and 100 nM concentrations of testosterone hormone and 10 nM dose of apelin-13 incubated for 24 h in the prostate cancer cells were administrated and were allowed to incubate for 24 h. Effects of apelin-13 and testosterone on prostate cancer cells were determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. At the end of the experiment, DU-145 did not affected cell viability while the treatment with apelin -13 and testosterone increased the viability of LNCaP cells (p<0.001). The proliferation effect was strongly occured when LNCaP cells were treated with together apelin-13 and testosterone (p<0.001) but, no effect was observed in DU-145 cells. In conclusion; apelin-13 had similar proliferative effects with testosterone on LNCaP cells, but had no effect on DU-145 cells. These results suggest that the effects of apelin-13 are similar to testosterone, and it realises their effects through an androgen receptor-dependent mechanism.

 

Key Words: Apelin, testosterone, prostate cancer, LNCaP, DU-145

Özet

Prostat kanseri erkeklerde görülen en yaygın kanser tiplerinden biridir. Değişik tipteki çoğu kanser türleri için kesin çözüm olabilecek bir tedavi yöntemi tam olarak geliştirilememiştir. Yağ dokudan salgılanan apelin, APJ reseptörüne bağlanarak etkilerini ortaya koymaktadır. Artan yağ doku kitlesi ile obezite gelişmekte ve obeziteye bağlı apelin salgısının da arttığı bilinmektedir. Obezitenin kanser gelişiminde bir risk faktörü olması, hem apelinin hem de reseptörünün testis ve prostat dokusunda yaygın bir şekilde bulunması apelinin prostat kanseri üzerinde önemli etkilerinin olabileceğini akla getirmektedir. Bu çalışmada ilk olarak, apelin-13’ün 0.1, 1 ve 10 nM'lik konsantrasyonları, testosteron hormonunun ise 1, 10, 100 nM’lik konsantrasyonları LNCaP (androjen reseptör pozitif) ve DU-145 (androjen reseptör negatif) insan prostat kanseri hücre serilerine ayrı ayrı uygulandı. Daha sonra testosteron hormonunun 1, 10, 100 nM’lik konsantrasyonları ile 24 saat inkübe edilen prostat kanser hücrelerine apelin-13’ün 10 nM’lik dozu uygulandı ve 24 saat inkübasyona bırakıldı. Apelin-13 ve testosteron hormonlarının prostat kanseri hücre canlılığı üzerine etkileri, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay ile belirlendi. Çalışma sonunda uygulanan apelin-13 ve testosteronun LNCaP hücre canlılığını arttırdığı (p<0.001), DU-145 hücre canlılığını ise etkilemediği belirlendi. Apelin-13 ile testosteronun birlikte uygulandığı LNCaP hücre serilerinde, sadece apelin ve testosteron uygulamalarına kıyasla daha güçlü bir proliferatif etki meydana geldiği (p<0.001) ancak DU-145 hücrelerinde herhangi bir etkinin olmadığı gözlendi. Sonuç olarak, apelin-13, LNCaP hücre serilerinde testosteronun etkisine benzer şekilde poliferasyona sebep olurken, DU-145 hücreleri üzerine herhangi bir etki göstermedi. Bu sonuçlar, apelin-13’ün etkilerini testosteronun etkisine benzer şekilde androjen reseptör bağımlı bir mekanizma üzerinden ortaya koyduğuna delil teşkil etmektedir.

 

Anahtar Kelimeler: Apelin, testosteron, prostat kanseri, LNCaP, DU-145

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Makale detayları

Effects of Apelin-13 on Human Prostate Cancer Lines [İnsan Prostat Kanseri Hücre Serilerinde Apelin-13’ün Etkileri]

Yazarlar: Suat TEKİN | Suleyman SANDAL | Cemil COLAK

Yayıncı: Inonu University

Dergi: Medicine Science 2014 Vol.3 Issue 3, pp.1427-1441

Belge Tipi: Makale

Yayın Tarihi: 9/2014

Belge Dili: Türkçe

Document ID (Org No.): 2000828458

Sıklık: Üç Aylık

eISSN: 2147-0634

pISSN: 2147-0634

Apelin

testosteron

prostat kanseri

LNCaP

DU-145

Sayı

Yazarlar:

Suat TEKİN | Suleyman SANDAL | Cemil COLAK

Yayıncı:

Inonu University

Dergi:

Medicine Science 2014 Vol.3 Issue 3, pp.1427-1441

Belge Tipi:

Makale

Yayın Tarihi:

9/2014

Belge Dili:

Türkçe

Document ID (Org No.):

2000828458

Sıklık:

Üç Aylık

eISSN:

2147-0634

pISSN:

2147-0634