Role of CAPE on Protein Profile and Oxidative Stress Panel in Healthy Liver Tissue

Seher Elif Koçoğlu; Leyla Çimen; Nur Tufanoğlu; Semih Yılmaz; Aysun Çetin

Makale detayları

Role of CAPE on Protein Profile and Oxidative Stress Panel in Healthy Liver Tissue

Yazarlar:

Seher Elif Koçoğlu | Leyla Çimen | Nur Tufanoğlu | Semih Yılmaz | Aysun Çetin

Yayıncı:

Dokuz Eylul University

Ana Başlıklar:

Analytical Biochemistry | Enzymes | Medical Biochemistry and Metabolomics (Other) | Biologically Active Molecules | Proteins and Peptides

Dergi:

Journal of Basic and Clinical Health Sciences Vol.9 Issue 3, pp.457-464

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001359212

pISSN:

2564-7288

Anahtar kelimeler:

Özet:

 Purpose: We aimed to investigate the effects of caffeic acid phenethyl ester CAPE, one of the active ingredients of propolis, on protein expression differences and some antioxidant parameters in rat liver. Methods: 3 groups were formed as control ip SF, CAPE 10 ip 10 μmolkg CAPE and CAPE 50 ip 10 μmolkg CAPE for 5 days. Protein expression differences were analyzed by SDS-PAGE gel electrophoresis, GSH-Px, SOD enzyme activities and MDA levels were measured by ELISA. Results: The amount of protein with a molecular weight MW of 53 kDa in CAPE 10 was found to be higher than control group p0.05. The amount of protein with a MW of 35 kDa in CAPE 10 group was found to be higher than control p0.001. The amount of protein with a MW of 14 kDa in CAPE 50 group was found to be higher than control p0.05. MDA levels were statistically higher in CAPE groups compared to control group p0.001. GSH-Px and SOD activities in CAPE groups was found to be higher than control group, and the difference was not statistically significant. Conclusions: CAPE didnt show the expected antioxidant effect on liver tissues, a prooxidant effect was observed.

 Purpose: We aimed to investigate the effects of caffeic acid phenethyl ester CAPE, one of the active ingredients of propolis, on protein expression differences and some antioxidant parameters in rat liver. Methods: 3 groups were formed as control ip SF, CAPE 10 ip 10 μmolkg CAPE and CAPE 50 ip 10 μmolkg CAPE for 5 days. Protein expression differences were analyzed by SDS-PAGE gel electrophoresis, GSH-Px, SOD enzyme activities and MDA levels were measured by ELISA. Results: The amount of protein with a molecular weight MW of 53 kDa in CAPE 10 was found to be higher than control group p0.05. The amount of protein with a MW of 35 kDa in CAPE 10 group was found to be higher than control p0.001. The amount of protein with a MW of 14 kDa in CAPE 50 group was found to be higher than control p0.05. MDA levels were statistically higher in CAPE groups compared to control group p0.001. GSH-Px and SOD activities in CAPE groups was found to be higher than control group, and the difference was not statistically significant. Conclusions: CAPE didnt show the expected antioxidant effect on liver tissues, a prooxidant effect was observed.

 Purpose: We aimed to investigate the effects of caffeic acid phenethyl ester CAPE, one of the active ingredients of propolis, on protein expression differences and some antioxidant parameters in rat liver. Methods: 3 groups were formed as control ip SF, CAPE 10 ip 10 μmolkg CAPE and CAPE 50 ip 10 μmolkg CAPE for 5 days. Protein expression differences were analyzed by SDS-PAGE gel electrophoresis, GSH-Px, SOD enzyme activities and MDA levels were measured by ELISA. Results: The amount of protein with a molecular weight MW of 53 kDa in CAPE 10 was found to be higher than control group p0.05. The amount of protein with a MW of 35 kDa in CAPE 10 group was found to be higher than control p0.001. The amount of protein with a MW of 14 kDa in CAPE 50 group was found to be higher than control p0.05. MDA levels were statistically higher in CAPE groups compared to control group p0.001. GSH-Px and SOD activities in CAPE groups was found to be higher than control group, and the difference was not statistically significant. Conclusions: CAPE didnt show the expected antioxidant effect on liver tissues, a prooxidant effect was observed.

 Purpose: We aimed to investigate the effects of caffeic acid phenethyl ester CAPE, one of the active ingredients of propolis, on protein expression differences and some antioxidant parameters in rat liver. Methods: 3 groups were formed as control ip SF, CAPE 10 ip 10 μmolkg CAPE and CAPE 50 ip 10 μmolkg CAPE for 5 days. Protein expression differences were analyzed by SDS-PAGE gel electrophoresis, GSH-Px, SOD enzyme activities and MDA levels were measured by ELISA. Results: The amount of protein with a molecular weight MW of 53 kDa in CAPE 10 was found to be higher than control group p0.05. The amount of protein with a MW of 35 kDa in CAPE 10 group was found to be higher than control p0.001. The amount of protein with a MW of 14 kDa in CAPE 50 group was found to be higher than control p0.05. MDA levels were statistically higher in CAPE groups compared to control group p0.001. GSH-Px and SOD activities in CAPE groups was found to be higher than control group, and the difference was not statistically significant. Conclusions: CAPE didnt show the expected antioxidant effect on liver tissues, a prooxidant effect was observed.

Makale detayları

Role of CAPE on Protein Profile and Oxidative Stress Panel in Healthy Liver Tissue

Yazarlar:

Seher Elif Koçoğlu | Leyla Çimen | Nur Tufanoğlu | Semih Yılmaz | Aysun Çetin

Yayıncı:

Dokuz Eylul University

Ana Başlıklar:

Analytical Biochemistry | Enzymes | Medical Biochemistry and Metabolomics (Other) | Biologically Active Molecules | Proteins and Peptides

Dergi:

Journal of Basic and Clinical Health Sciences Vol.9 Issue 3, pp.457-464

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001359212

pISSN:

2564-7288

propolis

caffeic acid phenethyl ester

liver

prooxidant

antioxidant

oxidative stress

Sayı

Makale detayları

Role of CAPE on Protein Profile and Oxidative Stress Panel in Healthy Liver Tissue

Yazarlar: Seher Elif Koçoğlu | Leyla Çimen | Nur Tufanoğlu | Semih Yılmaz | Aysun Çetin

Yayıncı: Dokuz Eylul University

Ana Başlıklar: Analytical Biochemistry | Enzymes | Medical Biochemistry and Metabolomics (Other) | Biologically Active Molecules | Proteins and Peptides

Dergi: Journal of Basic and Clinical Health Sciences Vol.9 Issue 3, pp.457-464

Belge Tipi: Makale

Yayın Tarihi: 9/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001359212

pISSN: 2564-7288

propolis

caffeic acid phenethyl ester

liver

prooxidant

antioxidant

oxidative stress

Sayı

Yazarlar:

Seher Elif Koçoğlu | Leyla Çimen | Nur Tufanoğlu | Semih Yılmaz | Aysun Çetin

Yayıncı:

Dokuz Eylul University

Ana Başlıklar:

Analytical Biochemistry | Enzymes | Medical Biochemistry and Metabolomics (Other) | Biologically Active Molecules | Proteins and Peptides

Dergi:

Journal of Basic and Clinical Health Sciences Vol.9 Issue 3, pp.457-464

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001359212

pISSN:

2564-7288