Investigation of the effect of quercetin on the accumulation of lipids and the level of adiponectin in 3T3-L1 adipocytes

Gizem Akan; Melek Tunç Ata; Emine Kılıç Toprak

Makale detayları

Investigation of the effect of quercetin on the accumulation of lipids and the level of adiponectin in 3T3-L1 adipocytes

Yazarlar:

Gizem Akan | Melek Tunç Ata | Emine Kılıç Toprak

Yayıncı:

Pamukkale University

Ana Başlıklar:

Tıbbi Fizyoloji (Diğer)

Dergi:

Pamukkale Tıp Dergisi Vol.18 Issue 1, pp.179-187

Belge Tipi:

Makale

Yayın Tarihi:

1/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001318851

pISSN:

1308-0865

Anahtar kelimeler:

Özet:

 Abstract Purpose: Obesity disrupts the homeostasis of adipose tissue, leading to an increase in the number and size of adipose cells. Many positive effects of quercetin polyphenol on health through various mechanisms are known in the literature. The aim of our study was to investigate the effects of quercetin on lipid accumulation and adiponectin ADP levels in mature and hypertrophic adipocytes. Materials and methods: In this study, 3T3-L1 differentiated preadipocyte cells were exposed to high glucose media for 8 days to generate mature adipocytes, then for 18 days to produce hypertrophic adipocytes. Quercetin 40 and 80 μM was administered to mature adipocytes for 24 hours and to hypertrophic adipocytes for 48 hours. Lipid content was visualized by Oil-Red-O staining method. Lipid accumulation and ADP level were measured by ELISA method. For statistical analysis, one-way analysis of variance post hoc: Tukey test was used. p0.05 was considered statistically significant. Results: The administration of both quercetin doses to mature adipocytes significantly reduced lipid accumulation p0.0001. The administration of 80 μM quercetin in mature adipocytes caused a significant increase in ADP levels p0.0001. Administration of quercetin to hypertrophic adipocytes caused a significant decrease in ADP levels p0.0001. Conclusion: Our study revealed that quercetin decreased lipid accumulation and increased ADP levels in mature adipocytes. However, in hypertrophic adipocytes, quercetin had no significant effect on lipid accumulation and decreased ADP levels. These findings suggest that quercetin has protective effects on health in the early stages of obesity, but its efficacy is limited in the later stages of obesity.

 Abstract Purpose: Obesity disrupts the homeostasis of adipose tissue, leading to an increase in the number and size of adipose cells. Many positive effects of quercetin polyphenol on health through various mechanisms are known in the literature. The aim of our study was to investigate the effects of quercetin on lipid accumulation and adiponectin ADP levels in mature and hypertrophic adipocytes. Materials and methods: In this study, 3T3-L1 differentiated preadipocyte cells were exposed to high glucose media for 8 days to generate mature adipocytes, then for 18 days to produce hypertrophic adipocytes. Quercetin 40 and 80 μM was administered to mature adipocytes for 24 hours and to hypertrophic adipocytes for 48 hours. Lipid content was visualized by Oil-Red-O staining method. Lipid accumulation and ADP level were measured by ELISA method. For statistical analysis, one-way analysis of variance post hoc: Tukey test was used. p0.05 was considered statistically significant. Results: The administration of both quercetin doses to mature adipocytes significantly reduced lipid accumulation p0.0001. The administration of 80 μM quercetin in mature adipocytes caused a significant increase in ADP levels p0.0001. Administration of quercetin to hypertrophic adipocytes caused a significant decrease in ADP levels p0.0001. Conclusion: Our study revealed that quercetin decreased lipid accumulation and increased ADP levels in mature adipocytes. However, in hypertrophic adipocytes, quercetin had no significant effect on lipid accumulation and decreased ADP levels. These findings suggest that quercetin has protective effects on health in the early stages of obesity, but its efficacy is limited in the later stages of obesity.

 Abstract Purpose: Obesity disrupts the homeostasis of adipose tissue, leading to an increase in the number and size of adipose cells. Many positive effects of quercetin polyphenol on health through various mechanisms are known in the literature. The aim of our study was to investigate the effects of quercetin on lipid accumulation and adiponectin ADP levels in mature and hypertrophic adipocytes. Materials and methods: In this study, 3T3-L1 differentiated preadipocyte cells were exposed to high glucose media for 8 days to generate mature adipocytes, then for 18 days to produce hypertrophic adipocytes. Quercetin 40 and 80 μM was administered to mature adipocytes for 24 hours and to hypertrophic adipocytes for 48 hours. Lipid content was visualized by Oil-Red-O staining method. Lipid accumulation and ADP level were measured by ELISA method. For statistical analysis, one-way analysis of variance post hoc: Tukey test was used. p0.05 was considered statistically significant. Results: The administration of both quercetin doses to mature adipocytes significantly reduced lipid accumulation p0.0001. The administration of 80 μM quercetin in mature adipocytes caused a significant increase in ADP levels p0.0001. Administration of quercetin to hypertrophic adipocytes caused a significant decrease in ADP levels p0.0001. Conclusion: Our study revealed that quercetin decreased lipid accumulation and increased ADP levels in mature adipocytes. However, in hypertrophic adipocytes, quercetin had no significant effect on lipid accumulation and decreased ADP levels. These findings suggest that quercetin has protective effects on health in the early stages of obesity, but its efficacy is limited in the later stages of obesity.

 Amaç: Obezite, adipoz dokunun homeostazını bozarak adipoz hücrelerinin sayısında ve büyüklüğünde artışa neden olur. Literatürde quercetin polifenolünün çeşitli mekanizmalar aracılığıyla sağlık üzerinde pek çok olumlu etkileri bilinmektedir. Araştırmamızın amacı, quercetinin olgun ve hipertrofik adipositlerde lipit birikimi ve adiponektin ADP seviyeleri üzerindeki etkilerini araştırmaktır. Gereç ve yöntem: Çalışmada, 3T3-L1 preadiposit hücreler farklılaştırıldıktan sonra yüksek glikoz içeren ortamda 8 gün muamele edilerek olgun adipositler, 18 gün muamele edilerek ise hipertrofik adipositler elde edildi. Quercetin 40 ve 80 μM, olgun adipositlere 24 saat, hipertrofik adipositlere ise 48 saat boyunca uygulandı. Oil-Red-O boyama yöntemiyle lipit miktarı görüntülendi. ELISA yöntemi aracılığıyla lipit birikimi ve ADP seviyesi ölçüldü. İstatistiksel analiz için; tek yönlü varyans analizi post hoc: Tukey testi kullanıldı. p0,05 değeri istatistiksel olarak anlamlı kabul edildi. Bulgular: Olgun adipositlere her iki quercetin dozunun uygulaması lipit birikimini anlamlı olarak azalttı p0,0001. Olgun adipositlerde 80 μM quercetin uygulaması, ADP seviyelerinde anlamlı bir artışa yol açtı p0,0001. Hipertrofik adipositlere quercetin uygulaması ise, ADP seviyelerinde anlamlı bir azalmaya yol açtı p0,0001. Sonuç: Çalışmamız, quercetinin olgun adipositlerde lipit birikimini azaltıp ADP seviyesini artırdığını ortaya koydu. Ancak, hipertrofik adipositlerde ise quercetinin lipit birikiminde anlamlı bir etkisi olmayıp ADP seviyesini azalttığı gözlendi. Bu bulgular, quercetinin obezitenin erken döneminde sağlık üzerindeki koruyucu etkilerini ortaya koymakla birlikte, obezitenin daha ileri döneminde etkinliğinin sınırlı kaldığını göstermektedir.

Makale detayları

Investigation of the effect of quercetin on the accumulation of lipids and the level of adiponectin in 3T3-L1 adipocytes

Yazarlar:

Gizem Akan | Melek Tunç Ata | Emine Kılıç Toprak

Yayıncı:

Pamukkale University

Ana Başlıklar:

Tıbbi Fizyoloji (Diğer)

Dergi:

Pamukkale Tıp Dergisi Vol.18 Issue 1, pp.179-187

Belge Tipi:

Makale

Yayın Tarihi:

1/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001318851

pISSN:

1308-0865

Adiponectin

adipocyte

lipid accumulation

obesity

quercetin.Adiponektin

adiposit

lipit birikimi

obezite

quercetin

Sayı

Makale detayları

Yazarlar: Gizem Akan | Melek Tunç Ata | Emine Kılıç Toprak

Yayıncı: Pamukkale University

Ana Başlıklar: Tıbbi Fizyoloji (Diğer)

Dergi: Pamukkale Tıp Dergisi Vol.18 Issue 1, pp.179-187

Belge Tipi: Makale

Yayın Tarihi: 1/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001318851

pISSN: 1308-0865

Sayı

Yazarlar:

Gizem Akan | Melek Tunç Ata | Emine Kılıç Toprak

Yayıncı:

Pamukkale University

Ana Başlıklar:

Tıbbi Fizyoloji (Diğer)

Dergi:

Pamukkale Tıp Dergisi Vol.18 Issue 1, pp.179-187

Belge Tipi:

Makale

Yayın Tarihi:

1/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001318851

pISSN:

1308-0865