Effects of different extenders and additives on liquid storage of Awassi ram semen

Ömer VARIŞLI; Abdullah TAŞKIN; Numan AKYOL

Makale detayları

Effects of different extenders and additives on liquid storage of Awassi ram semen

Yazarlar:

Ömer VARIŞLI | Abdullah TAŞKIN | Numan AKYOL

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2018 Vol.42 Issue 4, pp.230-242

Belge Tipi:

Makale

Yayın Tarihi:

8/2018

Belge Dili:

İngilizce

Document ID (Org No.):

2000764563

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Özet:

 

 
                         The aim of this study was to evaluate the effects of different extenders and additives on ram semen during liquid storage and to 
demonstrate the role of oxidative stress parameters on this process. In the present study, ejaculates taken by artificial vagina twice a week 
from 4 rams during the breeding season were used. They were mixed and used if motility and viability were above 70% and there was 
a 95% intact acrosome. The semen specimens were diluted by Tris-citrate-glucose (TRIS), Tris-TES (TEST), HEPES-buffered Tyrode 
lactate (TL-HEPES), and Dulbecco?s phosphate-buffered saline (PBS) extenders supplemented with different additives [centrifuged egg 
yolk, Equex-STM, bovine serum albumin (BSA), and ethylenediaminetetraacetic acid (EDTA)]. The specimens were stored at 4 °C for 
up to 96 h and evaluated for motility, membrane integrity, acrosome integrity, mitochondrial membrane potential (MMP), and oxidative 
stress parameters. At the end of the 96-h storage, the highest sperm motility was 64.2 ± 3.7% (P < 0.05) and significant loss of sperm 
motility and membrane integrity were not detected in extender TEST-3, but the MMP rate significantly declined. Acrosome integrity 
was not affected by storage time or extender types. BSA and EDTA decreased lipid peroxidation (LPO) and total oxidant status (TOS), 
but did not positively affect motility or membrane integrity. As a result, TRIS, TEST, and TL-HEPES-3 were observed to provide better 
protection for ram semen during liquid storage at 4 °C than other extenders. The role of oxidative stress and MMP are considerable in 
liquid storage.

Makale detayları

Effects of different extenders and additives on liquid storage of Awassi ram semen

Yazarlar: Ömer VARIŞLI | Abdullah TAŞKIN | Numan AKYOL

Yayıncı: TUBITAK

Dergi: Turkish Journal of Veterinary and Animal Sciences 2018 Vol.42 Issue 4, pp.230-242

Belge Tipi: Makale

Yayın Tarihi: 8/2018

Belge Dili: İngilizce

Document ID (Org No.): 2000764563

Sıklık: İki Aylık

eISSN: 1303-6181

pISSN: 1300-0128

Sayı

Yazarlar:

Ömer VARIŞLI | Abdullah TAŞKIN | Numan AKYOL

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2018 Vol.42 Issue 4, pp.230-242

Belge Tipi:

Makale

Yayın Tarihi:

8/2018

Belge Dili:

İngilizce

Document ID (Org No.):

2000764563

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128