The potency of obestatin in improving kidney functions and apoptosis in rats with cisplatin-induced acute kidney injury

Zarife Özdemir Kumral; Mustafa Vezirhüyük; Zafer Kök; Naziye Özkan Yenal; Berrak Yegen; Mehmet Koç

Makale detayları

The potency of obestatin in improving kidney functions and apoptosis in rats with cisplatin-induced acute kidney injury

Yazarlar:

Zarife Özdemir Kumral | Mustafa Vezirhüyük | Zafer Kök | Naziye Özkan Yenal | Berrak Yegen | Mehmet Koç

Yayıncı:

Marmara University

Ana Başlıklar:

Clinical Pharmacy and Pharmacy Practice

Dergi:

Journal of Research in Pharmacy Vol.26 Issue 5, pp.1375-1385

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001347556

pISSN:

2630-6344

Anahtar kelimeler:

Özet:

 Cisplatin CP, which is the most commonly used anticancer agent to treat several solid tumors, may cause acute kidney injury AKI as the major limiting factor for its clinical use. Obestatin OB is a ghrelin gene-derived peptide produced in several tissues and has shown anti-oxidant, anti-apoptotic, and anti-inflammatory effects in many experimental models. This study investigated the effect of OB treatment on nephrotoxicity induced by CP. Rats were divided into 4 groups as two control 1 mlkg, saline, intraperitoneal ip, single dose and two CP-induced 7 mgkg, ip, single dose AKI groups 8 rats in each group. Immediately after the CP injection and the following two days, injections of OB 10 μgkg, ip were performed. Rats were decapitated at the end of 72 hours. Blood and kidney tissue samples were taken for biochemical and histopathological measurements. The results of the present study revealed that serum creatinine and BUN levels were significantly increased in the CP-induced AKI group when compared to the control group. Treatment with OB improved kidney functions and ameliorated renal oxidative injury and maintained oxidative balance in the CP-induced AKI model, which was revealed by elevated malondialdehyde and depleted glutathione levels. TUNEL scores also demonstrated that CP increased the apoptotic response, while OB treatment abolished it. CP-induced medullary and cortical injuries were also partially reversed by OB treatment. Thus, our findings show that OB alleviates CP-induced nephrotoxicity in rats through the abolishment of oxidative stress and apoptosis.

 Cisplatin CP, which is the most commonly used anticancer agent to treat several solid tumors, may cause acute kidney injury AKI as the major limiting factor for its clinical use. Obestatin OB is a ghrelin gene-derived peptide produced in several tissues and has shown anti-oxidant, anti-apoptotic, and anti-inflammatory effects in many experimental models. This study investigated the effect of OB treatment on nephrotoxicity induced by CP. Rats were divided into 4 groups as two control 1 mlkg, saline, intraperitoneal ip, single dose and two CP-induced 7 mgkg, ip, single dose AKI groups 8 rats in each group. Immediately after the CP injection and the following two days, injections of OB 10 μgkg, ip were performed. Rats were decapitated at the end of 72 hours. Blood and kidney tissue samples were taken for biochemical and histopathological measurements. The results of the present study revealed that serum creatinine and BUN levels were significantly increased in the CP-induced AKI group when compared to the control group. Treatment with OB improved kidney functions and ameliorated renal oxidative injury and maintained oxidative balance in the CP-induced AKI model, which was revealed by elevated malondialdehyde and depleted glutathione levels. TUNEL scores also demonstrated that CP increased the apoptotic response, while OB treatment abolished it. CP-induced medullary and cortical injuries were also partially reversed by OB treatment. Thus, our findings show that OB alleviates CP-induced nephrotoxicity in rats through the abolishment of oxidative stress and apoptosis.

 Cisplatin CP, which is the most commonly used anticancer agent to treat several solid tumors, may cause acute kidney injury AKI as the major limiting factor for its clinical use. Obestatin OB is a ghrelin gene-derived peptide produced in several tissues and has shown anti-oxidant, anti-apoptotic, and anti-inflammatory effects in many experimental models. This study investigated the effect of OB treatment on nephrotoxicity induced by CP. Rats were divided into 4 groups as two control 1 mlkg, saline, intraperitoneal ip, single dose and two CP-induced 7 mgkg, ip, single dose AKI groups 8 rats in each group. Immediately after the CP injection and the following two days, injections of OB 10 μgkg, ip were performed. Rats were decapitated at the end of 72 hours. Blood and kidney tissue samples were taken for biochemical and histopathological measurements. The results of the present study revealed that serum creatinine and BUN levels were significantly increased in the CP-induced AKI group when compared to the control group. Treatment with OB improved kidney functions and ameliorated renal oxidative injury and maintained oxidative balance in the CP-induced AKI model, which was revealed by elevated malondialdehyde and depleted glutathione levels. TUNEL scores also demonstrated that CP increased the apoptotic response, while OB treatment abolished it. CP-induced medullary and cortical injuries were also partially reversed by OB treatment. Thus, our findings show that OB alleviates CP-induced nephrotoxicity in rats through the abolishment of oxidative stress and apoptosis.

 Cisplatin CP, which is the most commonly used anticancer agent to treat several solid tumors, may cause acute kidney injury AKI as the major limiting factor for its clinical use. Obestatin OB is a ghrelin gene-derived peptide produced in several tissues and has shown anti-oxidant, anti-apoptotic, and anti-inflammatory effects in many experimental models. This study investigated the effect of OB treatment on nephrotoxicity induced by CP. Rats were divided into 4 groups as two control 1 mlkg, saline, intraperitoneal ip, single dose and two CP-induced 7 mgkg, ip, single dose AKI groups 8 rats in each group. Immediately after the CP injection and the following two days, injections of OB 10 μgkg, ip were performed. Rats were decapitated at the end of 72 hours. Blood and kidney tissue samples were taken for biochemical and histopathological measurements. The results of the present study revealed that serum creatinine and BUN levels were significantly increased in the CP-induced AKI group when compared to the control group. Treatment with OB improved kidney functions and ameliorated renal oxidative injury and maintained oxidative balance in the CP-induced AKI model, which was revealed by elevated malondialdehyde and depleted glutathione levels. TUNEL scores also demonstrated that CP increased the apoptotic response, while OB treatment abolished it. CP-induced medullary and cortical injuries were also partially reversed by OB treatment. Thus, our findings show that OB alleviates CP-induced nephrotoxicity in rats through the abolishment of oxidative stress and apoptosis.

Makale detayları

The potency of obestatin in improving kidney functions and apoptosis in rats with cisplatin-induced acute kidney injury

Yazarlar: Zarife Özdemir Kumral | Mustafa Vezirhüyük | Zafer Kök | Naziye Özkan Yenal | Berrak Yegen | Mehmet Koç

Yayıncı: Marmara University

Ana Başlıklar: Clinical Pharmacy and Pharmacy Practice

Dergi: Journal of Research in Pharmacy Vol.26 Issue 5, pp.1375-1385

Belge Tipi: Makale

Yayın Tarihi: 6/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001347556

pISSN: 2630-6344

cisplatin

acute kidney injury

obestatin

rat

apoptosis

Sayı

Yazarlar:

Zarife Özdemir Kumral | Mustafa Vezirhüyük | Zafer Kök | Naziye Özkan Yenal | Berrak Yegen | Mehmet Koç

Yayıncı:

Marmara University

Ana Başlıklar:

Clinical Pharmacy and Pharmacy Practice

Dergi:

Journal of Research in Pharmacy Vol.26 Issue 5, pp.1375-1385

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001347556

pISSN:

2630-6344