Elevated Serum Levels of Inducible Nitric Oxide Synthase, Monocyte Chemoattractant Protein-1, And Cyclooxygenase-2 In Patients with Lung Cancer

Emine Yağcı; Cansu Özbayer; Güntülü Ak; Hülyam Kurt; Selma Metintaş; Muzaffer Metintaş

Makale detayları

Elevated Serum Levels of Inducible Nitric Oxide Synthase, Monocyte Chemoattractant Protein-1, And Cyclooxygenase-2 In Patients with Lung Cancer

Yazarlar:

Emine Yağcı | Cansu Özbayer | Güntülü Ak | Hülyam Kurt | Selma Metintaş | Muzaffer Metintaş

Yayıncı:

Eskişehir Osmangazi University

Ana Başlıklar:

Göğüs Hastalıkları | Tıbbi Genetik (Kanser Genetiği hariç)

Dergi:

Osmangazi Tıp Dergisi Vol.46 Issue 3, pp.388-393

Belge Tipi:

Makale

Yayın Tarihi:

5/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001313497

pISSN:

1305-4953

Anahtar kelimeler:

Özet:

 Lung cancer is a malignant lung tumor characterized by uncontrolled cell growth in lung tissue. Genetic and epigenetic abnormalities can be seen in lung cancer. These abnormalities can lead to activation of oncogene and inactivation of tumor suppressor genes. Inflammation is a powerful mediator of cancer development. Pulmonary inflammation may play a role in the initiation or progression of cancer. The main mediator of inflammation is inducible nitric oxide synthase iNOS, which synthesizes nitric oxide from L-arginine. Monocyte chemoattractant protein-1 MCP-1 is one of the important chemokines that regulate the migration and infiltration of monocytesmacrophages. It has been determined that MCP-1 plays an important role in lung allergic inflammation, lung leukocyte infiltration and bronchial hyperresponsiveness in the pathogenesis of asthma. Cyclooxygenases COX are responsible for prostaglandin production from arachidonic acid. They contribute to inflammation-induced carcinogenesis. COX2 is the enzyme responsible for inflammation induced by inflammatory stimuli, hormones and growth factors. In line with the information given, in this study, serum levels of COX2, iNOS and MCP-1 were determined using the ELISA method in 90 36 adenocarcinoma, 36 squamous cell, 18 small cell carcinoma lung cancer patients and 90 healthy control individuals. It was determined that COX2, iNOS and MCP-1 serum concentrations in lung cancer patients were significantly higher than in control individuals p0.001. However, no statistically significant difference was detected between lung cancer histological subtypes p0.05. It is thought that our findings may contribute to early diagnosis and development of new treatments for lung cancer.

 Lung cancer is a malignant lung tumor characterized by uncontrolled cell growth in lung tissue. Genetic and epigenetic abnormalities can be seen in lung cancer. These abnormalities can lead to activation of oncogene and inactivation of tumor suppressor genes. Inflammation is a powerful mediator of cancer development. Pulmonary inflammation may play a role in the initiation or progression of cancer. The main mediator of inflammation is inducible nitric oxide synthase iNOS, which synthesizes nitric oxide from L-arginine. Monocyte chemoattractant protein-1 MCP-1 is one of the important chemokines that regulate the migration and infiltration of monocytesmacrophages. It has been determined that MCP-1 plays an important role in lung allergic inflammation, lung leukocyte infiltration and bronchial hyperresponsiveness in the pathogenesis of asthma. Cyclooxygenases COX are responsible for prostaglandin production from arachidonic acid. They contribute to inflammation-induced carcinogenesis. COX2 is the enzyme responsible for inflammation induced by inflammatory stimuli, hormones and growth factors. In line with the information given, in this study, serum levels of COX2, iNOS and MCP-1 were determined using the ELISA method in 90 36 adenocarcinoma, 36 squamous cell, 18 small cell carcinoma lung cancer patients and 90 healthy control individuals. It was determined that COX2, iNOS and MCP-1 serum concentrations in lung cancer patients were significantly higher than in control individuals p0.001. However, no statistically significant difference was detected between lung cancer histological subtypes p0.05. It is thought that our findings may contribute to early diagnosis and development of new treatments for lung cancer.

 Lung cancer is a malignant lung tumor characterized by uncontrolled cell growth in lung tissue. Genetic and epigenetic abnormalities can be seen in lung cancer. These abnormalities can lead to activation of oncogene and inactivation of tumor suppressor genes. Inflammation is a powerful mediator of cancer development. Pulmonary inflammation may play a role in the initiation or progression of cancer. The main mediator of inflammation is inducible nitric oxide synthase iNOS, which synthesizes nitric oxide from L-arginine. Monocyte chemoattractant protein-1 MCP-1 is one of the important chemokines that regulate the migration and infiltration of monocytesmacrophages. It has been determined that MCP-1 plays an important role in lung allergic inflammation, lung leukocyte infiltration and bronchial hyperresponsiveness in the pathogenesis of asthma. Cyclooxygenases COX are responsible for prostaglandin production from arachidonic acid. They contribute to inflammation-induced carcinogenesis. COX2 is the enzyme responsible for inflammation induced by inflammatory stimuli, hormones and growth factors. In line with the information given, in this study, serum levels of COX2, iNOS and MCP-1 were determined using the ELISA method in 90 36 adenocarcinoma, 36 squamous cell, 18 small cell carcinoma lung cancer patients and 90 healthy control individuals. It was determined that COX2, iNOS and MCP-1 serum concentrations in lung cancer patients were significantly higher than in control individuals p0.001. However, no statistically significant difference was detected between lung cancer histological subtypes p0.05. It is thought that our findings may contribute to early diagnosis and development of new treatments for lung cancer.

 Akciğer kanseri, akciğer dokusunda kontrolsüz hücre büyümesi ile karakterize kötü huylu bir akciğer tümörüdür. Akciğer kanserinde genetik ve epigenetik anormallikler görülebilir. Bu anormallikler, onkogenin aktivasyonuna ve tümör supresör genlerin inaktivasyonuna neden olabilir. İnflamasyon kanser gelişiminde güçlü bir aracıdır, tümörün büyümesine ve vücuda yayılmasına yardımcı olur. İnflamatuar hücrelerden salınan reaktif azot veya oksijen türleri DNAya bağlanabilir ve genetik değişikliklere neden olabilir. Böylece, pulmoner inflamasyon kanserin başlamasında veya ilerlemesinde rol oynayabilir. İnflamasyonun ana aracısı, nitrik oksidi L-argininden sentezlenen indüklenebilir nitrik oksit sentazdır iNOS. iNOSun tümör ilerlemesindeki rolü çok karmaşıktır, hem promotör hem de inhibitör etkiler belirlenmiştir. Akut ve kronik inflamatuar reaksiyonlarda lökosit göçü ve kemokinlerin aktivasyonu önemli fonksiyonlardır. Monosit kemoatraktan protein-1 MCP-1, monositmakrofajların migrasyonunu ve infiltrasyonunu düzenleyen önemli kemokinlerden biridir. MCP-1in astım patogenezinde akciğer allerjik inflamasyonu, akciğer lökosit infiltrasyonu, bronş aşırı duyarlılığı ve eozinofillerin alınmasında önemli rol oynadığı tespit edilmiştir. Siklooksijenazlar COX, araşidonik asitten prostaglandin üretiminden sorumludur. Prostaglandinlerin sentezi inflamatuar bölgede önemli ölçüde artar. Ayrıca, inflamasyona bağlı karsinogeneze katkıda bulunurlar. Siklooksijenaz 2 COX2, inflamasyon uyaranları, hormonlar ve büyüme faktörleri tarafından indüklenen inflamasyondan sorumlu olan enzimdir. Verilen bilgiler doğrultusunda bu çalışmada, COX2, iNOS ve MCP-1in serum seviyeleri 90 36 adenokarsinom, 36 skuamöz hücreli, 18 küçük hücreli karsinom akciğer kanseri hastası ve 90 sağlıklı kontrol bireyde ELISA yöntemi kullanılarak belirlendi. Akciğer kanserli hastalarda COX2, iNOS ve MCP-1 serum konsantrasyonlarının kontrol bireylere göre anlamlı derecede yüksek olduğu belirlenmiştir p0.001. Ancak akciğer kanseri histolojik alt tipleri arasında istatistiksel olarak anlamlı fark tespit edilememiştir p0.05. Bulgularımızın akciğer kanserinde erken teşhise ve yeni tedavilerin geliştirilmesine katkıda bulunabileceği düşünülmektedir.

Makale detayları

Elevated Serum Levels of Inducible Nitric Oxide Synthase, Monocyte Chemoattractant Protein-1, And Cyclooxygenase-2 In Patients with Lung Cancer

Yazarlar:

Emine Yağcı | Cansu Özbayer | Güntülü Ak | Hülyam Kurt | Selma Metintaş | Muzaffer Metintaş

Yayıncı:

Eskişehir Osmangazi University

Ana Başlıklar:

Göğüs Hastalıkları | Tıbbi Genetik (Kanser Genetiği hariç)

Dergi:

Osmangazi Tıp Dergisi Vol.46 Issue 3, pp.388-393

Belge Tipi:

Makale

Yayın Tarihi:

5/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001313497

pISSN:

1305-4953

Lung Cancer

Inflammation

iNOS

MCP-1

COX2Akciğer Kanseri

İnflamasyon

iNOS

MCP-1

COX2

Sayı

Makale detayları

Yazarlar: Emine Yağcı | Cansu Özbayer | Güntülü Ak | Hülyam Kurt | Selma Metintaş | Muzaffer Metintaş

Yayıncı: Eskişehir Osmangazi University

Ana Başlıklar: Göğüs Hastalıkları | Tıbbi Genetik (Kanser Genetiği hariç)

Dergi: Osmangazi Tıp Dergisi Vol.46 Issue 3, pp.388-393

Belge Tipi: Makale

Yayın Tarihi: 5/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001313497

pISSN: 1305-4953

Sayı

Yazarlar:

Emine Yağcı | Cansu Özbayer | Güntülü Ak | Hülyam Kurt | Selma Metintaş | Muzaffer Metintaş

Yayıncı:

Eskişehir Osmangazi University

Ana Başlıklar:

Göğüs Hastalıkları | Tıbbi Genetik (Kanser Genetiği hariç)

Dergi:

Osmangazi Tıp Dergisi Vol.46 Issue 3, pp.388-393

Belge Tipi:

Makale

Yayın Tarihi:

5/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001313497

pISSN:

1305-4953