Acid-free synthesis of S-nitrosothiols at neutral pH by shock-freezing in liquid nitrogen

DIMITRIOS TSIKAS; Dimitrios TSIKAS; ANKE BÖHMER; ARNE TRETTIN; ARSLAN ARINÇ KAYAÇELEBİ

Makale detayları

Acid-free synthesis of S-nitrosothiols at neutral pH by shock-freezing in liquid nitrogen

Yazarlar:

DIMITRIOS TSIKAS | Dimitrios TSIKAS | ANKE BÖHMER | ARNE TRETTIN | ARSLAN ARINÇ KAYAÇELEBİ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Chemistry 2015 Vol.39 Issue 3, pp.676-682

Belge Tipi:

Makale

Yayın Tarihi:

8/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000726893

Sıklık:

İki Aylık

eISSN:

1303-6130

pISSN:

1300-0527

Özet:

 

A laboratory method for the acid-free synthesis of S-nitrosothiols (RSNO) from NaNO22_{2} and thiols in aqueous buffer of neutral pH is reported. Equimolar amounts of NaNO22_{2} and thiol are added to a 100-μμ\mu M Na44_{4}EDTA-containing Na22_{2}HPO44_{4} buffer (100 mM, pH 7.4). Samples are set into liquid nitrogen for 1 min and then thawed to room temperature or in an ice bath. Dependent upon the RSNO, repetition of the procedure may be required. This method may be of particular importance when the classical acid-based method fails or releases harmful gases such as H22_{2}S.

 

A laboratory method for the acid-free synthesis of S-nitrosothiols (RSNO) from NaNO22_{2} and thiols in aqueous buffer of neutral pH is reported. Equimolar amounts of NaNO22_{2} and thiol are added to a 100-μμ\mu M Na44_{4}EDTA-containing Na22_{2}HPO44_{4} buffer (100 mM, pH 7.4). Samples are set into liquid nitrogen for 1 min and then thawed to room temperature or in an ice bath. Dependent upon the RSNO, repetition of the procedure may be required. This method may be of particular importance when the classical acid-based method fails or releases harmful gases such as H22_{2}S.

Makale detayları

Acid-free synthesis of S-nitrosothiols at neutral pH by shock-freezing in liquid nitrogen

Yazarlar: DIMITRIOS TSIKAS | Dimitrios TSIKAS | ANKE BÖHMER | ARNE TRETTIN | ARSLAN ARINÇ KAYAÇELEBİ

Yayıncı: TUBITAK

Dergi: Turkish Journal of Chemistry 2015 Vol.39 Issue 3, pp.676-682

Belge Tipi: Makale

Yayın Tarihi: 8/2015

Belge Dili: Türkçe

Document ID (Org No.): 2000726893

Sıklık: İki Aylık

eISSN: 1303-6130

pISSN: 1300-0527

Sayı

Yazarlar:

DIMITRIOS TSIKAS | Dimitrios TSIKAS | ANKE BÖHMER | ARNE TRETTIN | ARSLAN ARINÇ KAYAÇELEBİ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Chemistry 2015 Vol.39 Issue 3, pp.676-682

Belge Tipi:

Makale

Yayın Tarihi:

8/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000726893

Sıklık:

İki Aylık

eISSN:

1303-6130

pISSN:

1300-0527