Thermostable a-amylase from moderately halophilic Halomonas sp. AAD21

Kamil Serkan UZYOL; Berna SARIYAR AKBULUT; Aziz Akın DENİZCİ; Dilek KAZAN

Makale detayları

Thermostable a-amylase from moderately halophilic Halomonas sp. AAD21

Yazarlar:

Kamil Serkan UZYOL | Berna SARIYAR AKBULUT | Aziz Akın DENİZCİ | Dilek KAZAN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2012 Vol.36 Issue 3, pp.327-338

Belge Tipi:

Makale

Yayın Tarihi:

2/2012

Belge Dili:

Türkçe

Document ID (Org No.):

2000812668

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Anahtar kelimeler:

Özet:

 

The moderately halophilic Halomonas sp. strain AAD21, which produces extracellular thermostable a-amylase, was isolated from the Çamaltı Saltern area located in İzmir, Turkey. NaCl, carbon, and nitrogen sources in the growth medium were optimized to enhance a-amylase yield. The highest enzyme yield was measured in the presence of 20% NaCl with peptone as the nitrogen and starch as the carbon sources in the fermentation broth. This microorganism was also found to utilize waste potato peel as a carbon source for a-amylase production. Concentrations of carbon and nitrogen sources were optimized using a statistical approach, and a-amylase activity increased from 4.07 U mL-1 min-1 to 26.25 U mL-1 min-1. Maximum a-amylase production was achieved at the end of 48 h of growth in the presence of 20% NaCl, 4.12% starch, 1.0% peptone, 0.2% KCl, 2% MgSO4.7H2O, and 0.03% trisodium citrate pentahydrate. The optimum pH and temperature of the a-amylase were found to be 7.0 and 50 °C, respectively. The a-amylase synthesized by Halomonas sp. AAD21 was also thermostable. Crude a-amylase did not lose its original activity after 2 h of incubation at 50 °C and 60 °C, and it retained 70% of its original activity after 120 min of incubation at 90 °C.

 

The moderately halophilic Halomonas sp. strain AAD21, which produces extracellular thermostable a-amylase, was isolated from the Çamaltı Saltern area located in İzmir, Turkey. NaCl, carbon, and nitrogen sources in the growth medium were optimized to enhance a-amylase yield. The highest enzyme yield was measured in the presence of 20% NaCl with peptone as the nitrogen and starch as the carbon sources in the fermentation broth. This microorganism was also found to utilize waste potato peel as a carbon source for a-amylase production. Concentrations of carbon and nitrogen sources were optimized using a statistical approach, and a-amylase activity increased from 4.07 U mL-1 min-1 to 26.25 U mL-1 min-1. Maximum a-amylase production was achieved at the end of 48 h of growth in the presence of 20% NaCl, 4.12% starch, 1.0% peptone, 0.2% KCl, 2% MgSO4.7H2O, and 0.03% trisodium citrate pentahydrate. The optimum pH and temperature of the a-amylase were found to be 7.0 and 50 °C, respectively. The a-amylase synthesized by Halomonas sp. AAD21 was also thermostable. Crude a-amylase did not lose its original activity after 2 h of incubation at 50 °C and 60 °C, and it retained 70% of its original activity after 120 min of incubation at 90 °C.

Makale detayları

Thermostable a-amylase from moderately halophilic Halomonas sp. AAD21

Yazarlar:

Kamil Serkan UZYOL | Berna SARIYAR AKBULUT | Aziz Akın DENİZCİ | Dilek KAZAN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2012 Vol.36 Issue 3, pp.327-338

Belge Tipi:

Makale

Yayın Tarihi:

2/2012

Belge Dili:

Türkçe

Document ID (Org No.):

2000812668

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Key words: Moderate halophile

Halomonas sp. AAD21

a-amylase

thermostable

optimization

Sayı

Makale detayları

Thermostable a-amylase from moderately halophilic Halomonas sp. AAD21

Yazarlar: Kamil Serkan UZYOL | Berna SARIYAR AKBULUT | Aziz Akın DENİZCİ | Dilek KAZAN

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2012 Vol.36 Issue 3, pp.327-338

Belge Tipi: Makale

Yayın Tarihi: 2/2012

Belge Dili: Türkçe

Document ID (Org No.): 2000812668

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Key words: Moderate halophile

Halomonas sp. AAD21

a-amylase

thermostable

optimization

Sayı

Yazarlar:

Kamil Serkan UZYOL | Berna SARIYAR AKBULUT | Aziz Akın DENİZCİ | Dilek KAZAN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2012 Vol.36 Issue 3, pp.327-338

Belge Tipi:

Makale

Yayın Tarihi:

2/2012

Belge Dili:

Türkçe

Document ID (Org No.):

2000812668

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152