Comparison of the ovine prion protein genotype profiles of breeds Tigaie with Black Head and Merino

Stelian BARAITAREANU; Maria Rodica OTELEA; Mihaela ZAULET; Kurt SCHNEIDER; Mihai TURCITU; Leonardo LEONARDI; Doina DANES

Makale detayları

Comparison of the ovine prion protein genotype profiles of breeds Tigaie with Black Head and Merino

Yazarlar:

Stelian BARAITAREANU | Maria Rodica OTELEA | Mihaela ZAULET | Kurt SCHNEIDER | Mihai TURCITU | Leonardo LEONARDI | Doina DANES

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2013 Vol.37 Issue 6, pp.625-630

Belge Tipi:

Makale

Yayın Tarihi:

5/2013

Belge Dili:

Türkçe

Document ID (Org No.):

2000766176

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Anahtar kelimeler:

Özet:

 

A comparison between a rare old breed and a common breed of ovine prion protein (PrP) genotype profiles was performed in 3 Romanian sheep flocks.  The total number of animals used in the research was 698 sheep, of which 53 were Merino breed (MB) rams, 12 were Tigaie with Black Head breed (TBHB) rams, 340 were MB ewes, and 293 were TBHB ewes. The PrP genotype was investigated by identification of codons 136, 154, and 171 and by gene sequencing between codons 101 and 227. All sheep groups contained the ARR, AHQ, ARH, ARQ, and VRQ haplotypes, with frequencies ranging from 1.37% (ARH in TBHB ewes) to 51.71% (ARQ in TBHB ewes). TBHB had a low prevalence of AHQ (2.05% in ewes, 4.17% in rams), ARH (1.37% in ewes, 4.17% in rams), and VRQ (1.88% in ewes, 8.33% in rams). The highest haplotype in TBHB was ARQ (51.71% in ewes, 37.50% in rams). ARQ was the most frequent haplotype in the MB (49.41% in ewes, 42.45% in rams). MB had a low prevalence of AHQ (3.24% in ewes, 6.60% in rams), ARH (1.47% in ewes, 5.66% in rams), and VRQ (3.53% in ewes, 9.43% in rams). The results indicated that 66.66% of TBHB rams and 68.26% of TBHB ewes have scrapie-resistant genotypes, despite the absence of a genetic selection program for scrapie. The proportion of scrapie resistant alleles was higher in the TBHB rams than in the MB rams.

 

A comparison between a rare old breed and a common breed of ovine prion protein (PrP) genotype profiles was performed in 3 Romanian sheep flocks.  The total number of animals used in the research was 698 sheep, of which 53 were Merino breed (MB) rams, 12 were Tigaie with Black Head breed (TBHB) rams, 340 were MB ewes, and 293 were TBHB ewes. The PrP genotype was investigated by identification of codons 136, 154, and 171 and by gene sequencing between codons 101 and 227. All sheep groups contained the ARR, AHQ, ARH, ARQ, and VRQ haplotypes, with frequencies ranging from 1.37% (ARH in TBHB ewes) to 51.71% (ARQ in TBHB ewes). TBHB had a low prevalence of AHQ (2.05% in ewes, 4.17% in rams), ARH (1.37% in ewes, 4.17% in rams), and VRQ (1.88% in ewes, 8.33% in rams). The highest haplotype in TBHB was ARQ (51.71% in ewes, 37.50% in rams). ARQ was the most frequent haplotype in the MB (49.41% in ewes, 42.45% in rams). MB had a low prevalence of AHQ (3.24% in ewes, 6.60% in rams), ARH (1.47% in ewes, 5.66% in rams), and VRQ (3.53% in ewes, 9.43% in rams). The results indicated that 66.66% of TBHB rams and 68.26% of TBHB ewes have scrapie-resistant genotypes, despite the absence of a genetic selection program for scrapie. The proportion of scrapie resistant alleles was higher in the TBHB rams than in the MB rams.

Makale detayları

Comparison of the ovine prion protein genotype profiles of breeds Tigaie with Black Head and Merino

Yazarlar:

Stelian BARAITAREANU | Maria Rodica OTELEA | Mihaela ZAULET | Kurt SCHNEIDER | Mihai TURCITU | Leonardo LEONARDI | Doina DANES

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2013 Vol.37 Issue 6, pp.625-630

Belge Tipi:

Makale

Yayın Tarihi:

5/2013

Belge Dili:

Türkçe

Document ID (Org No.):

2000766176

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Key words: TSEs

PrP genotyping

scrapie

Sayı

Makale detayları

Comparison of the ovine prion protein genotype profiles of breeds Tigaie with Black Head and Merino

Yazarlar: Stelian BARAITAREANU | Maria Rodica OTELEA | Mihaela ZAULET | Kurt SCHNEIDER | Mihai TURCITU | Leonardo LEONARDI | Doina DANES

Yayıncı: TUBITAK

Dergi: Turkish Journal of Veterinary and Animal Sciences 2013 Vol.37 Issue 6, pp.625-630

Belge Tipi: Makale

Yayın Tarihi: 5/2013

Belge Dili: Türkçe

Document ID (Org No.): 2000766176

Sıklık: İki Aylık

eISSN: 1303-6181

pISSN: 1300-0128

Key words: TSEs

PrP genotyping

scrapie

Sayı

Yazarlar:

Stelian BARAITAREANU | Maria Rodica OTELEA | Mihaela ZAULET | Kurt SCHNEIDER | Mihai TURCITU | Leonardo LEONARDI | Doina DANES

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2013 Vol.37 Issue 6, pp.625-630

Belge Tipi:

Makale

Yayın Tarihi:

5/2013

Belge Dili:

Türkçe

Document ID (Org No.):

2000766176

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128