Effect of Thidiazuron on Shoot Regeneration from Different Explants of Lentil  (Lens culinaris Medik.) via Organogenesis

Khalid Mahmood KHAWAR; Cengiz SANCAK; Serkan URANBEY; Sebahattin ÖZCAN

Makale detayları

Effect of Thidiazuron on Shoot Regeneration from Different Explants of Lentil (Lens culinaris Medik.) via Organogenesis

Yazarlar:

Khalid Mahmood KHAWAR | Cengiz SANCAK | Serkan URANBEY | Sebahattin ÖZCAN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Botany 2004 Vol.28 Issue 4, pp.421-426

Belge Tipi:

Makale

Yayın Tarihi:

3/2004

Belge Dili:

Türkçe

Document ID (Org No.):

2000724113

Sıklık:

İki Aylık

eISSN:

1303-6106

pISSN:

1300-008X

Özet:

 

Thidiazuron (TDZ) is among the most active cytokinin like substances and induces greater in vitro shoot proliferation than many other cytokinins in many plant species. Leaf, stem, stem node and cotyledonary node explants of 2 extensively cultivated Turkish lentil cultivars, Ali Dayı and Kayı 91, were cultured on Murashige and Skoog (MS) media supplemented with various concentrations of TDZ. The present study was conducted to develop a rapid and efficient shoot regeneration system suitable for the transformation of lentil (Lens culinaris Medik.) using TDZ.  Cotyledonary nodes and stem nodes after the initial callus stage regenerated prolific adventitious shoots via organogenesis. Shoot or callus formation was not achieved from leaf or stem explants. DMSO as a solvent for TDZ was necrotic on plant tissues and therefore TDZ was dissolved in 50% ethanol to carry out the studies. Cotyledonary nodes showed a higher shoot formation capacity than stem nodes. MS medium supplemented with 0.25 mg/l TDZ produced the highest frequency of shoot formation from cotyledonary nodes in both genotypes. Regenerated shoots (10-20 mm long) rooted in MS medium containing 0.25 mg/l indole-3-butyric acid (IBA). Rooted plantlets were finally transferred to sand in pots.  Abbreviations: TDZ - thidiazuron [1 Phenyl 3-(1,2,3-thiadiazol -5YL) urea], IBA - indole-3-butyric acid, MS - Murashige and Skoog, DMSO - dimethylsulphoxide.

 

Thidiazuron (TDZ) is among the most active cytokinin like substances and induces greater in vitro shoot proliferation than many other cytokinins in many plant species. Leaf, stem, stem node and cotyledonary node explants of 2 extensively cultivated Turkish lentil cultivars, Ali Dayı and Kayı 91, were cultured on Murashige and Skoog (MS) media supplemented with various concentrations of TDZ. The present study was conducted to develop a rapid and efficient shoot regeneration system suitable for the transformation of lentil (Lens culinaris Medik.) using TDZ.  Cotyledonary nodes and stem nodes after the initial callus stage regenerated prolific adventitious shoots via organogenesis. Shoot or callus formation was not achieved from leaf or stem explants. DMSO as a solvent for TDZ was necrotic on plant tissues and therefore TDZ was dissolved in 50% ethanol to carry out the studies. Cotyledonary nodes showed a higher shoot formation capacity than stem nodes. MS medium supplemented with 0.25 mg/l TDZ produced the highest frequency of shoot formation from cotyledonary nodes in both genotypes. Regenerated shoots (10-20 mm long) rooted in MS medium containing 0.25 mg/l indole-3-butyric acid (IBA). Rooted plantlets were finally transferred to sand in pots.  Abbreviations: TDZ - thidiazuron [1 Phenyl 3-(1,2,3-thiadiazol -5YL) urea], IBA - indole-3-butyric acid, MS - Murashige and Skoog, DMSO - dimethylsulphoxide.

Makale detayları

Effect of Thidiazuron on Shoot Regeneration from Different Explants of Lentil (Lens culinaris Medik.) via Organogenesis

Yazarlar: Khalid Mahmood KHAWAR | Cengiz SANCAK | Serkan URANBEY | Sebahattin ÖZCAN

Yayıncı: TUBITAK

Dergi: Turkish Journal of Botany 2004 Vol.28 Issue 4, pp.421-426

Belge Tipi: Makale

Yayın Tarihi: 3/2004

Belge Dili: Türkçe

Document ID (Org No.): 2000724113

Sıklık: İki Aylık

eISSN: 1303-6106

pISSN: 1300-008X

Sayı

Yazarlar:

Khalid Mahmood KHAWAR | Cengiz SANCAK | Serkan URANBEY | Sebahattin ÖZCAN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Botany 2004 Vol.28 Issue 4, pp.421-426

Belge Tipi:

Makale

Yayın Tarihi:

3/2004

Belge Dili:

Türkçe

Document ID (Org No.):

2000724113

Sıklık:

İki Aylık

eISSN:

1303-6106

pISSN:

1300-008X