Identification of Self Incompatibility (S) Alleles in Turkish Apple Gene Sources using Allele-specific PCR

Merve Dilek Karataş; Nahid Hazrati; Canan Yüksel Özmen; Mohammad Hasanzadeh; Serdar Altıntaş; Mehmet Emin Akçay; Ali Ergül

Makale detayları

Identification of Self Incompatibility (S) Alleles in Turkish Apple Gene Sources using Allele-specific PCR

Yazarlar:

Merve Dilek Karataş | Nahid Hazrati | Canan Yüksel Özmen | Mohammad Hasanzadeh | Serdar Altıntaş | Mehmet Emin Akçay | Ali Ergül

Yayıncı:

Ankara University

Dergi:

Journal of Agricultural Sciences Vol.29 Issue 1, pp.287-297

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001282534

pISSN:

1300-7580

Anahtar kelimeler:

Özet:

 Self-incompatibility SI is a genetic mechanism in many flowering plants by which generative reproduction is prevented. The self-incompatibility caused by the genetic functions of the cell is controlled by genes called S genes or self-incompatibility genes. Self-incompatibility results in decreased pollination and ultimately yield loss. In apple Malus domestica L., self-incompatibility is controlled by multi-allelic S-locus. Approaches in the S-glycoprotein profiles and allele-specific PCR methods using the gene profiles and S-glycoprotein profiles for determination of the incompatibility levels are of great importance. In current study, the self-incompatibility status of 192 apple genotypes such as, Amasya, Hüryemez, Şah elması, Tokat, Demir elması etc. obtained from the National Collection of Atatürk Horticultural Central Research Institute, Yalova, Turkey, has been determined. For this purpose, genotype-specific allele status and compatibility levels were screened via PCR Polymerase Chain Reaction using 4 different S-alleles Sd, Sf, S26 and S9. 181 genotypes containing at least 1 S-allele were identified as Partially Incompatible and 12 genotypes involving 4 S-alleles were assigned Totally Incompatible. No S-alleles were observed in 2 genotypes Pancarlık and Hüryemez which exhibited Compatibility status.

 Self-incompatibility SI is a genetic mechanism in many flowering plants by which generative reproduction is prevented. The self-incompatibility caused by the genetic functions of the cell is controlled by genes called S genes or self-incompatibility genes. Self-incompatibility results in decreased pollination and ultimately yield loss. In apple Malus domestica L., self-incompatibility is controlled by multi-allelic S-locus. Approaches in the S-glycoprotein profiles and allele-specific PCR methods using the gene profiles and S-glycoprotein profiles for determination of the incompatibility levels are of great importance. In current study, the self-incompatibility status of 192 apple genotypes such as, Amasya, Hüryemez, Şah elması, Tokat, Demir elması etc. obtained from the National Collection of Atatürk Horticultural Central Research Institute, Yalova, Turkey, has been determined. For this purpose, genotype-specific allele status and compatibility levels were screened via PCR Polymerase Chain Reaction using 4 different S-alleles Sd, Sf, S26 and S9. 181 genotypes containing at least 1 S-allele were identified as Partially Incompatible and 12 genotypes involving 4 S-alleles were assigned Totally Incompatible. No S-alleles were observed in 2 genotypes Pancarlık and Hüryemez which exhibited Compatibility status.

 Self-incompatibility SI is a genetic mechanism in many flowering plants by which generative reproduction is prevented. The self-incompatibility caused by the genetic functions of the cell is controlled by genes called S genes or self-incompatibility genes. Self-incompatibility results in decreased pollination and ultimately yield loss. In apple Malus domestica L., self-incompatibility is controlled by multi-allelic S-locus. Approaches in the S-glycoprotein profiles and allele-specific PCR methods using the gene profiles and S-glycoprotein profiles for determination of the incompatibility levels are of great importance. In current study, the self-incompatibility status of 192 apple genotypes such as, Amasya, Hüryemez, Şah elması, Tokat, Demir elması etc. obtained from the National Collection of Atatürk Horticultural Central Research Institute, Yalova, Turkey, has been determined. For this purpose, genotype-specific allele status and compatibility levels were screened via PCR Polymerase Chain Reaction using 4 different S-alleles Sd, Sf, S26 and S9. 181 genotypes containing at least 1 S-allele were identified as Partially Incompatible and 12 genotypes involving 4 S-alleles were assigned Totally Incompatible. No S-alleles were observed in 2 genotypes Pancarlık and Hüryemez which exhibited Compatibility status.

 Self-incompatibility SI is a genetic mechanism in many flowering plants by which generative reproduction is prevented. The self-incompatibility caused by the genetic functions of the cell is controlled by genes called S genes or self-incompatibility genes. Self-incompatibility results in decreased pollination and ultimately yield loss. In apple Malus domestica L., self-incompatibility is controlled by multi-allelic S-locus. Approaches in the S-glycoprotein profiles and allele-specific PCR methods using the gene profiles and S-glycoprotein profiles for determination of the incompatibility levels are of great importance. In current study, the self-incompatibility status of 192 apple genotypes such as, Amasya, Hüryemez, Şah elması, Tokat, Demir elması etc. obtained from the National Collection of Atatürk Horticultural Central Research Institute, Yalova, Turkey, has been determined. For this purpose, genotype-specific allele status and compatibility levels were screened via PCR Polymerase Chain Reaction using 4 different S-alleles Sd, Sf, S26 and S9. 181 genotypes containing at least 1 S-allele were identified as Partially Incompatible and 12 genotypes involving 4 S-alleles were assigned Totally Incompatible. No S-alleles were observed in 2 genotypes Pancarlık and Hüryemez which exhibited Compatibility status.

Makale detayları

Identification of Self Incompatibility (S) Alleles in Turkish Apple Gene Sources using Allele-specific PCR

Yazarlar:

Merve Dilek Karataş | Nahid Hazrati | Canan Yüksel Özmen | Mohammad Hasanzadeh | Serdar Altıntaş | Mehmet Emin Akçay | Ali Ergül

Yayıncı:

Ankara University

Dergi:

Journal of Agricultural Sciences Vol.29 Issue 1, pp.287-297

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001282534

pISSN:

1300-7580

Malus domestica L.

Self-incompatibility

Anatolian gen resourses

S-alleles

Sayı

Makale detayları

Identification of Self Incompatibility (S) Alleles in Turkish Apple Gene Sources using Allele-specific PCR

Yazarlar: Merve Dilek Karataş | Nahid Hazrati | Canan Yüksel Özmen | Mohammad Hasanzadeh | Serdar Altıntaş | Mehmet Emin Akçay | Ali Ergül

Yayıncı: Ankara University

Dergi: Journal of Agricultural Sciences Vol.29 Issue 1, pp.287-297

Belge Tipi: Makale

Yayın Tarihi: 1/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001282534

pISSN: 1300-7580

Malus domestica L.

Self-incompatibility

Anatolian gen resourses

S-alleles

Sayı

Yazarlar:

Merve Dilek Karataş | Nahid Hazrati | Canan Yüksel Özmen | Mohammad Hasanzadeh | Serdar Altıntaş | Mehmet Emin Akçay | Ali Ergül

Yayıncı:

Ankara University

Dergi:

Journal of Agricultural Sciences Vol.29 Issue 1, pp.287-297

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001282534

pISSN:

1300-7580