Antibacterial compound of Bacillus Amyloliquefaciens and Bacillus Siamensis: screening, characterization, and evaluation

Rajendrabhai Vasaıt

Makale detayları

Antibacterial compound of Bacillus Amyloliquefaciens and Bacillus Siamensis: screening, characterization, and evaluation

Yazarlar:

Rajendrabhai Vasaıt

Yayıncı:

İzzet KARA

Ana Başlıklar:

Pharmacology and Pharmaceutical Sciences | Structural Biology | Organic Chemistry

Dergi:

International Journal of Secondary Metabolite Vol.10 Issue 2, pp.175-189

Belge Tipi:

Makale

Yayın Tarihi:

6/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001289449

pISSN:

2148-6905

Anahtar kelimeler:

Özet:

 This study was aimed at isolating potential antimicrobial compound AMC producing bacteria. AMC produced by a Bacillus species was evaluated further for its antimicrobial potential. Antimicrobial compound-producing bacteria were isolated from the soil of crop fields from the local region of Satana, Nashik India, and tested against clinical isolates. Both isolates exhibited remarkable antibacterial potential against Gram-positive and Gram-negative clinical isolates. The AMCs of both SYS 1 and SYS 2 exhibited excellent antibacterial activity against Salmonella paratyphi B and Staphylococcus aureus. Both AMC-producing isolates were characterized and identified. Bacillus species SYS 1 and SYS 2 were identified as Bacillus amyloliquefaciens SYS 1 and Bacillus siamensis SYS 2, respectively. The highest antimicrobial activity of AMC produced by Bacillus amyloliquefaciens SYS 1 was exhibited against Salmonella paratyphi B 28 mm, followed by Staphylococcus aureus 26 mm. Bacillus siamensis SYS 2 AMC extracted by the solvent ethyl acetate exerted the highest antimicrobial activity against Salmonella paratyphi B 18 mm, followed by Staphylococcus aureus 16 mm. A partial characterization of the AMC was conducted and evaluated to contain amino acids and proteins. A higher total protein content of 17.9 μgmL was estimated in the partially purified AMC of Bacillus amyloliquefaciens SYS 1. A detailed evaluation of the structural characteristics of AMC could prove its importance in commercial applications.

 This study was aimed at isolating potential antimicrobial compound AMC producing bacteria. AMC produced by a Bacillus species was evaluated further for its antimicrobial potential. Antimicrobial compound-producing bacteria were isolated from the soil of crop fields from the local region of Satana, Nashik India, and tested against clinical isolates. Both isolates exhibited remarkable antibacterial potential against Gram-positive and Gram-negative clinical isolates. The AMCs of both SYS 1 and SYS 2 exhibited excellent antibacterial activity against Salmonella paratyphi B and Staphylococcus aureus. Both AMC-producing isolates were characterized and identified. Bacillus species SYS 1 and SYS 2 were identified as Bacillus amyloliquefaciens SYS 1 and Bacillus siamensis SYS 2, respectively. The highest antimicrobial activity of AMC produced by Bacillus amyloliquefaciens SYS 1 was exhibited against Salmonella paratyphi B 28 mm, followed by Staphylococcus aureus 26 mm. Bacillus siamensis SYS 2 AMC extracted by the solvent ethyl acetate exerted the highest antimicrobial activity against Salmonella paratyphi B 18 mm, followed by Staphylococcus aureus 16 mm. A partial characterization of the AMC was conducted and evaluated to contain amino acids and proteins. A higher total protein content of 17.9 μgmL was estimated in the partially purified AMC of Bacillus amyloliquefaciens SYS 1. A detailed evaluation of the structural characteristics of AMC could prove its importance in commercial applications.

 This study was aimed at isolating potential antimicrobial compound AMC producing bacteria. AMC produced by a Bacillus species was evaluated further for its antimicrobial potential. Antimicrobial compound-producing bacteria were isolated from the soil of crop fields from the local region of Satana, Nashik India, and tested against clinical isolates. Both isolates exhibited remarkable antibacterial potential against Gram-positive and Gram-negative clinical isolates. The AMCs of both SYS 1 and SYS 2 exhibited excellent antibacterial activity against Salmonella paratyphi B and Staphylococcus aureus. Both AMC-producing isolates were characterized and identified. Bacillus species SYS 1 and SYS 2 were identified as Bacillus amyloliquefaciens SYS 1 and Bacillus siamensis SYS 2, respectively. The highest antimicrobial activity of AMC produced by Bacillus amyloliquefaciens SYS 1 was exhibited against Salmonella paratyphi B 28 mm, followed by Staphylococcus aureus 26 mm. Bacillus siamensis SYS 2 AMC extracted by the solvent ethyl acetate exerted the highest antimicrobial activity against Salmonella paratyphi B 18 mm, followed by Staphylococcus aureus 16 mm. A partial characterization of the AMC was conducted and evaluated to contain amino acids and proteins. A higher total protein content of 17.9 μgmL was estimated in the partially purified AMC of Bacillus amyloliquefaciens SYS 1. A detailed evaluation of the structural characteristics of AMC could prove its importance in commercial applications.

 This study was aimed at isolating potential antimicrobial compound AMC producing bacteria. AMC produced by a Bacillus species was evaluated further for its antimicrobial potential. Antimicrobial compound-producing bacteria were isolated from the soil of crop fields from the local region of Satana, Nashik India, and tested against clinical isolates. Both isolates exhibited remarkable antibacterial potential against Gram-positive and Gram-negative clinical isolates. The AMCs of both SYS 1 and SYS 2 exhibited excellent antibacterial activity against Salmonella paratyphi B and Staphylococcus aureus. Both AMC-producing isolates were characterized and identified. Bacillus species SYS 1 and SYS 2 were identified as Bacillus amyloliquefaciens SYS 1 and Bacillus siamensis SYS 2, respectively. The highest antimicrobial activity of AMC produced by Bacillus amyloliquefaciens SYS 1 was exhibited against Salmonella paratyphi B 28 mm, followed by Staphylococcus aureus 26 mm. Bacillus siamensis SYS 2 AMC extracted by the solvent ethyl acetate exerted the highest antimicrobial activity against Salmonella paratyphi B 18 mm, followed by Staphylococcus aureus 16 mm. A partial characterization of the AMC was conducted and evaluated to contain amino acids and proteins. A higher total protein content of 17.9 μgmL was estimated in the partially purified AMC of Bacillus amyloliquefaciens SYS 1. A detailed evaluation of the structural characteristics of AMC could prove its importance in commercial applications.

Makale detayları

Antibacterial compound of Bacillus Amyloliquefaciens and Bacillus Siamensis: screening, characterization, and evaluation

Yazarlar:

Rajendrabhai Vasaıt

Yayıncı:

İzzet KARA

Ana Başlıklar:

Pharmacology and Pharmaceutical Sciences | Structural Biology | Organic Chemistry

Dergi:

International Journal of Secondary Metabolite Vol.10 Issue 2, pp.175-189

Belge Tipi:

Makale

Yayın Tarihi:

6/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001289449

pISSN:

2148-6905

Antimicrobial compound

Bioactive compound

Antimicrobial activity

Bacillus amyloliquefaciens Bacillus siamensisAntimicrobial compound

Bioactive compound

Antimicrobial activity

Bacillus amyloliquefaciens Bacillus siamensis

Sayı

Makale detayları

Yazarlar: Rajendrabhai Vasaıt

Yayıncı: İzzet KARA

Ana Başlıklar: Pharmacology and Pharmaceutical Sciences | Structural Biology | Organic Chemistry

Dergi: International Journal of Secondary Metabolite Vol.10 Issue 2, pp.175-189

Belge Tipi: Makale

Yayın Tarihi: 6/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001289449

pISSN: 2148-6905

Sayı

Yazarlar:

Rajendrabhai Vasaıt

Yayıncı:

İzzet KARA

Ana Başlıklar:

Pharmacology and Pharmaceutical Sciences | Structural Biology | Organic Chemistry

Dergi:

International Journal of Secondary Metabolite Vol.10 Issue 2, pp.175-189

Belge Tipi:

Makale

Yayın Tarihi:

6/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001289449

pISSN:

2148-6905