Determination of Antimicrobial Activity of Commercially Sold Spirulina Platensis Extracts Obtained With Three Different Solvents against Selected Pathogenic Bacteria and Fungi

Aysel Veyisoğlu; Abdulğani Yeşilyer; Demet Tatar

Makale detayları

Determination of Antimicrobial Activity of Commercially Sold Spirulina Platensis Extracts Obtained With Three Different Solvents against Selected Pathogenic Bacteria and Fungi

Yazarlar:

Aysel Veyisoğlu | Abdulğani Yeşilyer | Demet Tatar

Yayıncı:

Karyay Karadeniz Yayımcılık

Ana Başlıklar:

Mikrobiyoloji (Diğer)

Dergi:

Black Sea Journal of Engineering and Science Vol.7 Issue 5, pp.1031-1035

Belge Tipi:

Makale

Yayın Tarihi:

9/2024

Belge Dili:

Türkçe

Document ID (Org No.):

2001324917

pISSN:

2619-8991

Anahtar kelimeler:

Özet:

 Members of the genus Spirulina, like many other species of cyanobacteria, are known to have the potential to produce numerous antimicrobial substances. In our study, extracts of the commercial powder form of Spirulina platensis were tested using three different solvents acetone, hexane, methanol against seven bacterial strains Bacillus cereus ATCC 10987, Bacillus subtilis ATCC 6623, Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 70060, Pseudomonas aeruginosa ATCC 27853 and two fungal species Candida albicans ATCC 10231, Aspergillus niger ATCC 16404 at five different concentrations 30 mgml, 15 mgml, 6 mgml, 3 mgml and 1 mgml using the disc diffusion method. According to our results, it was observed that the extract obtained from methanol gave the best result among the solvents used. The extract obtained from methanol showed antimicrobial activity against Bacillus cereus ATCC 10987, Bacillus subtilis ATCC 6623, Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 70060 and Pseudomonas aeruginosa ATCC 27853 strains, The extract obtained from hexane showed activity against Bacillus cereus ATCC 10987 and Bacillus subtilis ATCC 6623 strains, while the extract obtained from acetone showed antimicrobial activity only against Pseudomonas aeruginosa ATCC 27853 strain. The extracts obtained from methanol, acetone and hexane did not show activity against the fungi used.

 Members of the genus Spirulina, like many other species of cyanobacteria, are known to have the potential to produce numerous antimicrobial substances. In our study, extracts of the commercial powder form of Spirulina platensis were tested using three different solvents acetone, hexane, methanol against seven bacterial strains Bacillus cereus ATCC 10987, Bacillus subtilis ATCC 6623, Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 70060, Pseudomonas aeruginosa ATCC 27853 and two fungal species Candida albicans ATCC 10231, Aspergillus niger ATCC 16404 at five different concentrations 30 mgml, 15 mgml, 6 mgml, 3 mgml and 1 mgml using the disc diffusion method. According to our results, it was observed that the extract obtained from methanol gave the best result among the solvents used. The extract obtained from methanol showed antimicrobial activity against Bacillus cereus ATCC 10987, Bacillus subtilis ATCC 6623, Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 70060 and Pseudomonas aeruginosa ATCC 27853 strains, The extract obtained from hexane showed activity against Bacillus cereus ATCC 10987 and Bacillus subtilis ATCC 6623 strains, while the extract obtained from acetone showed antimicrobial activity only against Pseudomonas aeruginosa ATCC 27853 strain. The extracts obtained from methanol, acetone and hexane did not show activity against the fungi used.

 Members of the genus Spirulina, like many other species of cyanobacteria, are known to have the potential to produce numerous antimicrobial substances. In our study, extracts of the commercial powder form of Spirulina platensis were tested using three different solvents acetone, hexane, methanol against seven bacterial strains Bacillus cereus ATCC 10987, Bacillus subtilis ATCC 6623, Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 70060, Pseudomonas aeruginosa ATCC 27853 and two fungal species Candida albicans ATCC 10231, Aspergillus niger ATCC 16404 at five different concentrations 30 mgml, 15 mgml, 6 mgml, 3 mgml and 1 mgml using the disc diffusion method. According to our results, it was observed that the extract obtained from methanol gave the best result among the solvents used. The extract obtained from methanol showed antimicrobial activity against Bacillus cereus ATCC 10987, Bacillus subtilis ATCC 6623, Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 70060 and Pseudomonas aeruginosa ATCC 27853 strains, The extract obtained from hexane showed activity against Bacillus cereus ATCC 10987 and Bacillus subtilis ATCC 6623 strains, while the extract obtained from acetone showed antimicrobial activity only against Pseudomonas aeruginosa ATCC 27853 strain. The extracts obtained from methanol, acetone and hexane did not show activity against the fungi used.

 Spirulina cinsi üyelerinin, diğer birçok siyanobakteri türü gibi çok sayıda antimikrobiyal madde üretme potansiyeline sahip olduğu bilinmektedir. Çalışmamızda, ticari olarak satılan toz formundaki Spirulina platensisin üç farklı çözücü aseton, hekzan, metanol kullanılarak elde edilen ekstraktlarının yedi bakteri suşu Bacillus cereus ATCC 10987, Bacillus subtilis ATCC 6623, Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 70060, Pseudomonas aeruginosa ATCC 27853 ve iki fungus türüne Candida albicans ATCC 10231, Aspergillus niger ATCC 16404 karşı beş farklı konsantrasyonda 30 mgml, 15 mgml, 6 mgml, 3 mgml ve 1 mgml disk difüzyon yöntemi kullanılarak antimikrobiyal aktivitesinin belirlenmesi amaçlanmıştır.Sonuçlarımıza göre, kullanılan çözücüler arasında en iyi sonucu metanolden elde edilen ekstraktın verdiği görülmüştür. Metanolden elde edilen ekstrakt Bacillus cereus ATCC 10987, Bacillus subtilis ATCC 6623, Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 70060 ve Pseudomonas aeruginosa ATCC 27853 suşlarına karşı antimikrobiyal aktivite gösterirken, hekzandan elde edilen ekstrakt Bacillus cereus ATCC 10987 ve Bacillus subtilis ATCC 6623 suşlarına karşı aktivite göstermiş, asetondan elde edilen ekstrakt ise sadece Pseudomonas aeruginosa ATCC 27853 suşuna karşı antimikrobiyal aktivite göstermiştir. Metanol, aseton ve hekzandan elde edilen ekstraktlar kullanılan funguslara karşı aktivite göstermemiştir.

Makale detayları

Determination of Antimicrobial Activity of Commercially Sold Spirulina Platensis Extracts Obtained With Three Different Solvents against Selected Pathogenic Bacteria and Fungi

Yazarlar:

Aysel Veyisoğlu | Abdulğani Yeşilyer | Demet Tatar

Yayıncı:

Karyay Karadeniz Yayımcılık

Ana Başlıklar:

Mikrobiyoloji (Diğer)

Dergi:

Black Sea Journal of Engineering and Science Vol.7 Issue 5, pp.1031-1035

Belge Tipi:

Makale

Yayın Tarihi:

9/2024

Belge Dili:

Türkçe

Document ID (Org No.):

2001324917

pISSN:

2619-8991

Spirulina platensis

Antimicrobial activity

Extract

Disc diffusionSpirulina platensis

Antimikrobiyal aktivite

Ekstrakt

Disk difüzyon

Sayı

Makale detayları

Yazarlar: Aysel Veyisoğlu | Abdulğani Yeşilyer | Demet Tatar

Yayıncı: Karyay Karadeniz Yayımcılık

Ana Başlıklar: Mikrobiyoloji (Diğer)

Dergi: Black Sea Journal of Engineering and Science Vol.7 Issue 5, pp.1031-1035

Belge Tipi: Makale

Yayın Tarihi: 9/2024

Belge Dili: Türkçe

Document ID (Org No.): 2001324917

pISSN: 2619-8991

Sayı

Yazarlar:

Aysel Veyisoğlu | Abdulğani Yeşilyer | Demet Tatar

Yayıncı:

Karyay Karadeniz Yayımcılık

Ana Başlıklar:

Mikrobiyoloji (Diğer)

Dergi:

Black Sea Journal of Engineering and Science Vol.7 Issue 5, pp.1031-1035

Belge Tipi:

Makale

Yayın Tarihi:

9/2024

Belge Dili:

Türkçe

Document ID (Org No.):

2001324917

pISSN:

2619-8991