Effects of diclofenac sodium exposure during pregnancy on COX-2 expression in the ductus epididymis and ductus deferens of the offspring rat

Oğuzhan Kuşcu; Fikret Altındağ; Rahmi Aslan; Murat Çetin Rağbetli

Makale detayları

Effects of diclofenac sodium exposure during pregnancy on COX-2 expression in the ductus epididymis and ductus deferens of the offspring rat

Yazarlar:

Oğuzhan Kuşcu | Fikret Altındağ | Rahmi Aslan | Murat Çetin Rağbetli

Yayıncı:

Ondokuz Mayıs University

Ana Başlıklar:

Histology and Embryology

Dergi:

Deneysel ve Klinik Tıp Dergisi Vol.41 Issue 1, pp.160-165

Belge Tipi:

Makale

Yayın Tarihi:

3/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001314476

pISSN:

1309-4483

Anahtar kelimeler:

Özet:

 The present study aimed to investigate the effects of diclofenac sodium application during pregnancy on COX-2 expression in the ductus epididymis DE and ductus deferens DD of the offspring rat. 8 adult Wistar Albino non-pregnant female rats and two breeding male rats were utilized. Rats determined to be pregnant were separated into two categories as sham S and diclofenac sodium DS. It was injected into the S group with 1 mlkgday saline for 10 days, and it was injected into the DS group with 6.1 mgkgday DS. 7 male offspring were used in each group. DS decreased the serum testosterone levels and incresed the number of mast. DS induced fibrotic thickening. Offspring of S group had moderate COX-2 in the caput epididymis CPE and severe COX-2 in the cauda epididymis CE, while those in the DS group had weak COX-2 in the CPE and moderate COX-2 in the CE. There was no COX-2 expression in the vas deferens and corpus epididymis COE of the offspring rats in the S and DS groups. TNF-α expression was not observed in the S and DS groups. It was observed that moderate IL-10 expression in the CPE and CE, and weak IL-10 expression in the vas deferens and COE in the S and DS groups. The findings of our study reveal that DS administration during pregnancy can cause the downregulation of COX-2 expression in the DE and DD of offspring rats and therefore may have adverse effects on the male reproductive system.

 The present study aimed to investigate the effects of diclofenac sodium application during pregnancy on COX-2 expression in the ductus epididymis DE and ductus deferens DD of the offspring rat. 8 adult Wistar Albino non-pregnant female rats and two breeding male rats were utilized. Rats determined to be pregnant were separated into two categories as sham S and diclofenac sodium DS. It was injected into the S group with 1 mlkgday saline for 10 days, and it was injected into the DS group with 6.1 mgkgday DS. 7 male offspring were used in each group. DS decreased the serum testosterone levels and incresed the number of mast. DS induced fibrotic thickening. Offspring of S group had moderate COX-2 in the caput epididymis CPE and severe COX-2 in the cauda epididymis CE, while those in the DS group had weak COX-2 in the CPE and moderate COX-2 in the CE. There was no COX-2 expression in the vas deferens and corpus epididymis COE of the offspring rats in the S and DS groups. TNF-α expression was not observed in the S and DS groups. It was observed that moderate IL-10 expression in the CPE and CE, and weak IL-10 expression in the vas deferens and COE in the S and DS groups. The findings of our study reveal that DS administration during pregnancy can cause the downregulation of COX-2 expression in the DE and DD of offspring rats and therefore may have adverse effects on the male reproductive system.

 The present study aimed to investigate the effects of diclofenac sodium application during pregnancy on COX-2 expression in the ductus epididymis DE and ductus deferens DD of the offspring rat. 8 adult Wistar Albino non-pregnant female rats and two breeding male rats were utilized. Rats determined to be pregnant were separated into two categories as sham S and diclofenac sodium DS. It was injected into the S group with 1 mlkgday saline for 10 days, and it was injected into the DS group with 6.1 mgkgday DS. 7 male offspring were used in each group. DS decreased the serum testosterone levels and incresed the number of mast. DS induced fibrotic thickening. Offspring of S group had moderate COX-2 in the caput epididymis CPE and severe COX-2 in the cauda epididymis CE, while those in the DS group had weak COX-2 in the CPE and moderate COX-2 in the CE. There was no COX-2 expression in the vas deferens and corpus epididymis COE of the offspring rats in the S and DS groups. TNF-α expression was not observed in the S and DS groups. It was observed that moderate IL-10 expression in the CPE and CE, and weak IL-10 expression in the vas deferens and COE in the S and DS groups. The findings of our study reveal that DS administration during pregnancy can cause the downregulation of COX-2 expression in the DE and DD of offspring rats and therefore may have adverse effects on the male reproductive system.

 The present study aimed to investigate the effects of diclofenac sodium application during pregnancy on COX-2 expression in the ductus epididymis DE and ductus deferens DD of the offspring rat. 8 adult Wistar Albino non-pregnant female rats and two breeding male rats were utilized. Rats determined to be pregnant were separated into two categories as sham S and diclofenac sodium DS. It was injected into the S group with 1 mlkgday saline for 10 days, and it was injected into the DS group with 6.1 mgkgday DS. 7 male offspring were used in each group. DS decreased the serum testosterone levels and incresed the number of mast. DS induced fibrotic thickening. Offspring of S group had moderate COX-2 in the caput epididymis CPE and severe COX-2 in the cauda epididymis CE, while those in the DS group had weak COX-2 in the CPE and moderate COX-2 in the CE. There was no COX-2 expression in the vas deferens and corpus epididymis COE of the offspring rats in the S and DS groups. TNF-α expression was not observed in the S and DS groups. It was observed that moderate IL-10 expression in the CPE and CE, and weak IL-10 expression in the vas deferens and COE in the S and DS groups. The findings of our study reveal that DS administration during pregnancy can cause the downregulation of COX-2 expression in the DE and DD of offspring rats and therefore may have adverse effects on the male reproductive system.

Makale detayları

Effects of diclofenac sodium exposure during pregnancy on COX-2 expression in the ductus epididymis and ductus deferens of the offspring rat

Yazarlar:

Oğuzhan Kuşcu | Fikret Altındağ | Rahmi Aslan | Murat Çetin Rağbetli

Yayıncı:

Ondokuz Mayıs University

Ana Başlıklar:

Histology and Embryology

Dergi:

Deneysel ve Klinik Tıp Dergisi Vol.41 Issue 1, pp.160-165

Belge Tipi:

Makale

Yayın Tarihi:

3/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001314476

pISSN:

1309-4483

COX-2

Diclofenac sodium

Ductus epididymis

Rat

Vas deferens.

Sayı

Makale detayları

Effects of diclofenac sodium exposure during pregnancy on COX-2 expression in the ductus epididymis and ductus deferens of the offspring rat

Yazarlar: Oğuzhan Kuşcu | Fikret Altındağ | Rahmi Aslan | Murat Çetin Rağbetli

Yayıncı: Ondokuz Mayıs University

Ana Başlıklar: Histology and Embryology

Dergi: Deneysel ve Klinik Tıp Dergisi Vol.41 Issue 1, pp.160-165

Belge Tipi: Makale

Yayın Tarihi: 3/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001314476

pISSN: 1309-4483

COX-2

Diclofenac sodium

Ductus epididymis

Rat

Vas deferens.

Sayı

Yazarlar:

Oğuzhan Kuşcu | Fikret Altındağ | Rahmi Aslan | Murat Çetin Rağbetli

Yayıncı:

Ondokuz Mayıs University

Ana Başlıklar:

Histology and Embryology

Dergi:

Deneysel ve Klinik Tıp Dergisi Vol.41 Issue 1, pp.160-165

Belge Tipi:

Makale

Yayın Tarihi:

3/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001314476

pISSN:

1309-4483