RİFAMPİSİN KAYNAKLI BEYİN DOKUSU HASARINDA LİNALOOL’UN İYİLEŞTİRİCİ RÖLÜ

Meltem Özgöçmen; Sebile Azırak

Makale detayları

RİFAMPİSİN KAYNAKLI BEYİN DOKUSU HASARINDA LİNALOOL’UN İYİLEŞTİRİCİ RÖLÜ

Yazarlar:

Meltem Özgöçmen | Sebile Azırak

Yayıncı:

Süleyman Demirel University

Ana Başlıklar:

Clinical Sciences

Dergi:

Medical Journal of Süleyman Demirel University Vol.30 Issue 3, pp.362-370

Belge Tipi:

Makale

Yayın Tarihi:

9/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001299110

pISSN:

1300-7416

Anahtar kelimeler:

Özet:

 Objective In this study linalool LN, which has antihyperglycemic, hypolipidemic and antioxidant properties, is intended to be used in the treatment of neurodegenerations and neural disorders that may occur due to rifampicin RF. For this reason, it was aimed to examine the effects of LN on the expression of genes, biochemical and histopathological parameters in these metabolic pathways against neurotoxicity that may occur due to RF, and to investigate the protective effects of LN, which has antioxidant properties. Material and Method Thirty healthy male Spraque-Dawley rats were divided into five groups group 1; control, group 2; solvent control DMSO; group 3, RF; group 4, LN; group 5; RFLN. Brain tissues were taken for biochemical, histological and gene expressions analyses. Blood samples were taken to measure blood glucose levels. Results Rifampicin treatment significantly increased CYP1A1 and CYP1A2 mRNA gene expression and blood glucose levels, while reducing brain weight according to findings. On the other hand, there was a significant decrease in CYP1A1 and CYP1A2 mRNA gene expression and blood glucose levels in the RFLN group, while a significant improvement in brain weight was observed and as a result of histological analyzes, it was observed that the damage caused by RF decreased in the groups given LN. Conclusion LN was found to be highly effective in protecting the brain from the toxic effects of RF.

 Objective In this study linalool LN, which has antihyperglycemic, hypolipidemic and antioxidant properties, is intended to be used in the treatment of neurodegenerations and neural disorders that may occur due to rifampicin RF. For this reason, it was aimed to examine the effects of LN on the expression of genes, biochemical and histopathological parameters in these metabolic pathways against neurotoxicity that may occur due to RF, and to investigate the protective effects of LN, which has antioxidant properties. Material and Method Thirty healthy male Spraque-Dawley rats were divided into five groups group 1; control, group 2; solvent control DMSO; group 3, RF; group 4, LN; group 5; RFLN. Brain tissues were taken for biochemical, histological and gene expressions analyses. Blood samples were taken to measure blood glucose levels. Results Rifampicin treatment significantly increased CYP1A1 and CYP1A2 mRNA gene expression and blood glucose levels, while reducing brain weight according to findings. On the other hand, there was a significant decrease in CYP1A1 and CYP1A2 mRNA gene expression and blood glucose levels in the RFLN group, while a significant improvement in brain weight was observed and as a result of histological analyzes, it was observed that the damage caused by RF decreased in the groups given LN. Conclusion LN was found to be highly effective in protecting the brain from the toxic effects of RF.

 Objective In this study linalool LN, which has antihyperglycemic, hypolipidemic and antioxidant properties, is intended to be used in the treatment of neurodegenerations and neural disorders that may occur due to rifampicin RF. For this reason, it was aimed to examine the effects of LN on the expression of genes, biochemical and histopathological parameters in these metabolic pathways against neurotoxicity that may occur due to RF, and to investigate the protective effects of LN, which has antioxidant properties. Material and Method Thirty healthy male Spraque-Dawley rats were divided into five groups group 1; control, group 2; solvent control DMSO; group 3, RF; group 4, LN; group 5; RFLN. Brain tissues were taken for biochemical, histological and gene expressions analyses. Blood samples were taken to measure blood glucose levels. Results Rifampicin treatment significantly increased CYP1A1 and CYP1A2 mRNA gene expression and blood glucose levels, while reducing brain weight according to findings. On the other hand, there was a significant decrease in CYP1A1 and CYP1A2 mRNA gene expression and blood glucose levels in the RFLN group, while a significant improvement in brain weight was observed and as a result of histological analyzes, it was observed that the damage caused by RF decreased in the groups given LN. Conclusion LN was found to be highly effective in protecting the brain from the toxic effects of RF.

 Amaç Bu çalışmada antihiperglisemik, hipolipidemik ve antioksidan özelliklere sahip olan linalool LN, rifampisine RF bağlı olarak oluşabilen nörodejenerasyonlar ve nöral bozuklukların tedavisi amacıyla kullanılmıştır. Bu amaçla, RFye bağlı oluşabilecek nörotoksisitede, antioksidan özelliği olan LNnin koruyucu etkinliği hem gen ekspresyonu seviyesinde hem de biyokimyasal ve histopatolojik bulgularla araştırılmıştır. Gereç ve Yöntem 30 adet sağlıklı erkek Spraque-Dawley rat beş gruba ayrıldı Grup 1: Kontrol grubu; Grup 2: Çözücü Kontrol Dimetilsülfoksit-DMSO grubu; Grup 3: Rifampisin RF grubu; Grup 4: Linalool LN grubu; Grup 5: Rifampisin-Linalool RFLN grubu. Biyokimyasal ve histolojik analizler ile gen ekspresyon seviyeleri için beyin dokuları, kan glikoz seviyelerini ölçmek için kan örnekleri alındı. Bulgular Rifampisin uygulaması, beyin ağırlığını azaltırken, CYP1A1 ve CYP1A2 mRNA gen ekspresyonunu ve kan glukoz düzeylerini önemli ölçüde artırmıştır. Diğer yandan RFLN grubunda CYP1A1 ve CYP1A2 mRNA gen ekspresyonu ve kan glukoz düzeylerinde anlamlı azalmalar olurken, beyin ağırlığında anlamlı düzelme gözlenmiştir. Histolojik bulgular LN verilen gruplarda, RF ye bağlı oluşan hasarların azaldığını göstermiştir. Sonuç LNnin beyin dokusunu RFnin toksik etkilerinden korumada oldukça etkili olduğu tespit edilmiştir.

Makale detayları

RİFAMPİSİN KAYNAKLI BEYİN DOKUSU HASARINDA LİNALOOL’UN İYİLEŞTİRİCİ RÖLÜ

Yazarlar:

Meltem Özgöçmen | Sebile Azırak

Yayıncı:

Süleyman Demirel University

Ana Başlıklar:

Clinical Sciences

Dergi:

Medical Journal of Süleyman Demirel University Vol.30 Issue 3, pp.362-370

Belge Tipi:

Makale

Yayın Tarihi:

9/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001299110

pISSN:

1300-7416

CYP1A2

Linalool

Brain

CYP1A1

Rifampicin.CYP1A2

Linalool

Rifampisin

Beyin

CYP1A1

Sayı

Makale detayları

Yazarlar: Meltem Özgöçmen | Sebile Azırak

Yayıncı: Süleyman Demirel University

Ana Başlıklar: Clinical Sciences

Dergi: Medical Journal of Süleyman Demirel University Vol.30 Issue 3, pp.362-370

Belge Tipi: Makale

Yayın Tarihi: 9/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001299110

pISSN: 1300-7416

Sayı

Yazarlar:

Meltem Özgöçmen | Sebile Azırak

Yayıncı:

Süleyman Demirel University

Ana Başlıklar:

Clinical Sciences

Dergi:

Medical Journal of Süleyman Demirel University Vol.30 Issue 3, pp.362-370

Belge Tipi:

Makale

Yayın Tarihi:

9/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001299110

pISSN:

1300-7416