Effects of Interleukin-33 on Neopterin and IP-10 Production in Macrophages

Rahime Aksoy Bulgurcu

Makale detayları

Effects of Interleukin-33 on Neopterin and IP-10 Production in Macrophages

Yazarlar:

Rahime Aksoy Bulgurcu

Yayıncı:

Ankara University

Ana Başlıklar:

Haematology

Dergi:

Ankara Üniversitesi Tıp Fakültesi Mecmuası Vol.76 Issue 1, pp.30-36

Belge Tipi:

Makale

Yayın Tarihi:

5/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001349824

pISSN:

0365-8104

Anahtar kelimeler:

Özet:

 Objectives: Interleukin-33 IL-33 is a pleiotropic cytokine from the IL-1 family. It is known to cause plasticity in the Th2 direction. The macrophages, important cells of the innate immune system, express the IL-33 receptor ST2L and exhibit M2 macrophage polarity under the influence of IL-33. Macrophages produce interferon gamma-stimulated protein 10 IP-10, an interferon-γ-induced chemokine, and neopterin which exhibits cellular immunoreaction. The aim of this study was to investigate the effects of IL-33 on neopterin and IP-10 production in unstimulated and prestimulated macrophages with different cytokines. Materials and Methods: The J774.1 macrophage cell line was used. J774.1 macrophages were stimulated by IFN-γ 40 UmL  LPS 10 ngmL after pretreatment with IL-33 100 ngmL. Nitrit levels were measured by the Griess reaction. To examine the effect of IL-33 on polarized macrophages, J774.1 macrophages were stimulated with IL-4, IL-10 ve IL-13 cytokines to provide M1, M2a and M2c macrophage polarization. Neopterin and IP-10 measurements were carried out by ELISA method. Statistical analysis was performed with SPSS 18 software. Results: IL-33 was found to significantly increase neopterin production in unstimulated and plasticity-affected J774.1 cells. There was no change in IP-10 levels in all experiments. No correlation was found between neopterin and IP-10 levels. Conclusion: Our present study is the first study on the effects of IL-33 cytokine on neopterin production. IL-33 causes a significant increase in neopterin production in J774.1 cells.

 Objectives: Interleukin-33 IL-33 is a pleiotropic cytokine from the IL-1 family. It is known to cause plasticity in the Th2 direction. The macrophages, important cells of the innate immune system, express the IL-33 receptor ST2L and exhibit M2 macrophage polarity under the influence of IL-33. Macrophages produce interferon gamma-stimulated protein 10 IP-10, an interferon-γ-induced chemokine, and neopterin which exhibits cellular immunoreaction. The aim of this study was to investigate the effects of IL-33 on neopterin and IP-10 production in unstimulated and prestimulated macrophages with different cytokines. Materials and Methods: The J774.1 macrophage cell line was used. J774.1 macrophages were stimulated by IFN-γ 40 UmL  LPS 10 ngmL after pretreatment with IL-33 100 ngmL. Nitrit levels were measured by the Griess reaction. To examine the effect of IL-33 on polarized macrophages, J774.1 macrophages were stimulated with IL-4, IL-10 ve IL-13 cytokines to provide M1, M2a and M2c macrophage polarization. Neopterin and IP-10 measurements were carried out by ELISA method. Statistical analysis was performed with SPSS 18 software. Results: IL-33 was found to significantly increase neopterin production in unstimulated and plasticity-affected J774.1 cells. There was no change in IP-10 levels in all experiments. No correlation was found between neopterin and IP-10 levels. Conclusion: Our present study is the first study on the effects of IL-33 cytokine on neopterin production. IL-33 causes a significant increase in neopterin production in J774.1 cells.

 Objectives: Interleukin-33 IL-33 is a pleiotropic cytokine from the IL-1 family. It is known to cause plasticity in the Th2 direction. The macrophages, important cells of the innate immune system, express the IL-33 receptor ST2L and exhibit M2 macrophage polarity under the influence of IL-33. Macrophages produce interferon gamma-stimulated protein 10 IP-10, an interferon-γ-induced chemokine, and neopterin which exhibits cellular immunoreaction. The aim of this study was to investigate the effects of IL-33 on neopterin and IP-10 production in unstimulated and prestimulated macrophages with different cytokines. Materials and Methods: The J774.1 macrophage cell line was used. J774.1 macrophages were stimulated by IFN-γ 40 UmL  LPS 10 ngmL after pretreatment with IL-33 100 ngmL. Nitrit levels were measured by the Griess reaction. To examine the effect of IL-33 on polarized macrophages, J774.1 macrophages were stimulated with IL-4, IL-10 ve IL-13 cytokines to provide M1, M2a and M2c macrophage polarization. Neopterin and IP-10 measurements were carried out by ELISA method. Statistical analysis was performed with SPSS 18 software. Results: IL-33 was found to significantly increase neopterin production in unstimulated and plasticity-affected J774.1 cells. There was no change in IP-10 levels in all experiments. No correlation was found between neopterin and IP-10 levels. Conclusion: Our present study is the first study on the effects of IL-33 cytokine on neopterin production. IL-33 causes a significant increase in neopterin production in J774.1 cells.

 Amaç: Testis torsiyonu, çocuk cerrahisi ve çocuk üroloji pratiğinde yaygın görülen cerrahi acildir. Ameliyatın zamanlaması ve torsiyon derecesi testis kurtarılmasını etkileyen faktörlerdir. Bu çalışmanın amacı, deneysel bir sıçan testis torsiyon modelinde Pentraxin 3 PTX 3 düzeylerinin tanısal bir belirteç olarak etkinliğini araştırmaktır. Gereç ve Yöntem: Toplam 17 Wistar albino erkek sıçan rastgele olarak yedisi Sham grubu Grup S, onu testiküler torsiyon grubu Grup TT olmak üzere 2 gruba ayrıldı. Grup Sde 4. saatte, Grup TTde sırasıyla 4., 24. ve 72. saatlerde kan alındı. Histopatolojik değerlendirme yapıldı. PTX 3 seviyeleri her sıçan için ölçüldü. Bulgular: Grup Snin PTX 3 medyan plazma seviyesi 1.409 ngmL ve grup TTnin 4., 24. ve 72. saatlerindeki PTX 3 medyan plazma seviyeleri sırasıyla 1.393 ngmL, 1.283 ngmL, 1.094 ngmL olarak bulundu. Grup TTnin 72. saat ölçümleri ile Grup Snin ölçümleri arasında istatistiksel olarak anlamlı fark vardı p0,004. Sonuç: Deneysel sıçan testis tosiyon modelinde 72. saatte PTX 3 seviyeleri düştüğü gözlendi. Ancak testislerin korunması için tanı, semptomların başlamasından itibaren 6 saat içinde konulmalıdır. PTX 3 seviyeleri, testis torsiyonunun erken teşhisi için yararlı bir belirteç olarak görülmemektedir.

Makale detayları

Effects of Interleukin-33 on Neopterin and IP-10 Production in Macrophages

Yazarlar:

Rahime Aksoy Bulgurcu

Yayıncı:

Ankara University

Ana Başlıklar:

Haematology

Dergi:

Ankara Üniversitesi Tıp Fakültesi Mecmuası Vol.76 Issue 1, pp.30-36

Belge Tipi:

Makale

Yayın Tarihi:

5/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001349824

pISSN:

0365-8104

Interleukin-33

IP-10

Neopterin

Macrophage

ELISABiyokimyasal Marker

Pentraxin 3

Sıçan Modeli

Testis Torsiyonu

Sayı

Makale detayları

Yazarlar: Rahime Aksoy Bulgurcu

Yayıncı: Ankara University

Ana Başlıklar: Haematology

Dergi: Ankara Üniversitesi Tıp Fakültesi Mecmuası Vol.76 Issue 1, pp.30-36

Belge Tipi: Makale

Yayın Tarihi: 5/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001349824

pISSN: 0365-8104

Sayı

Yazarlar:

Rahime Aksoy Bulgurcu

Yayıncı:

Ankara University

Ana Başlıklar:

Haematology

Dergi:

Ankara Üniversitesi Tıp Fakültesi Mecmuası Vol.76 Issue 1, pp.30-36

Belge Tipi:

Makale

Yayın Tarihi:

5/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001349824

pISSN:

0365-8104