Characterization, Identification and Phylogeny of the Creatine Kinase (ckma) Gene in Medaka (Oryzias latipes)

Mehtap BAYIR; Gökhan ARSLAN; Pinar OĞUZHAN YILDIZ

doi:https://doi.org/10.33714/masteb.660220

Makale detayları

Characterization, Identification and Phylogeny of the Creatine Kinase (ckma) Gene in Medaka (Oryzias latipes)

Yazarlar:

Mehtap BAYIR | Gökhan ARSLAN | Pinar OĞUZHAN YILDIZ

Yayıncı:

Adem Yavuz SÖNMEZ

Ana Başlıklar:

Ekoloji

Dergi:

Marine Science and Technology Bulletin 2020 Vol.9 Issue 1, pp.15-22

Belge Tipi:

Makale

Yayın Tarihi:

6/2020

Belge Dili:

İngilizce

Document ID (Org No.):

2000543445

Sıklık:

Üç Aylık

eISSN:

2147-9666

DOI:

https://doi.org/10.33714/masteb.660220

Özet:

 


Creatine kinase (ckma) has been characterized and described in
the medaka (Oryzias latipes), an aquatic model organism and the gene
structure has been designed using the exons, introns, produced amino acids of
the gene, TATA box, poly A tail and 5’ UTR and 3’ UTR regions of the ckma
gene. In another step, firstly, the chromosome region of the ckma gene
was determined in medaka and then the other genes which placed in the same
region were determined. Then the locations of these genes were determined in
zebrafish and human which are the orthologs of medaka. Finally, the conserved
gene synteny was designed manually, using these data. However, genetic identity
and similarity ratio between medaka and its orthologs were calculated. In this
study, characterization and identification, phylogenetic relationship,
conserved gene synteny of ckma gene in medaka (O. latipes) which
is an important model organism were analyzed by using bioinformatics tools
(NCBI database, Ensembl genomic database, Expasy, Reverse Complementary and
some programs such as MEGA6 program, BLOSUM62 matrix program and BioEdit
software). All these data will be used in future studies on molecular stress
response in fish and they were presented to the scientific world with this
study.


.

Makale detayları

Characterization, Identification and Phylogeny of the Creatine Kinase (ckma) Gene in Medaka (Oryzias latipes)

Yazarlar: Mehtap BAYIR | Gökhan ARSLAN | Pinar OĞUZHAN YILDIZ

Yayıncı: Adem Yavuz SÖNMEZ

Ana Başlıklar: Ekoloji

Dergi: Marine Science and Technology Bulletin 2020 Vol.9 Issue 1, pp.15-22

Belge Tipi: Makale

Yayın Tarihi: 6/2020

Belge Dili: İngilizce

Document ID (Org No.): 2000543445

Sıklık: Üç Aylık

eISSN: 2147-9666

DOI: https://doi.org/10.33714/masteb.660220

Sayı

Yazarlar:

Mehtap BAYIR | Gökhan ARSLAN | Pinar OĞUZHAN YILDIZ

Yayıncı:

Adem Yavuz SÖNMEZ

Ana Başlıklar:

Ekoloji

Dergi:

Marine Science and Technology Bulletin 2020 Vol.9 Issue 1, pp.15-22

Belge Tipi:

Makale

Yayın Tarihi:

6/2020

Belge Dili:

İngilizce

Document ID (Org No.):

2000543445

Sıklık:

Üç Aylık

eISSN:

2147-9666

DOI:

https://doi.org/10.33714/masteb.660220