The Effects of a Probiotic (Bacillus clausii) in Acute Kidney Injury in a Rat Model of LPS-Induced Endotoxemia

Asli KANDİL

Makale detayları

The Effects of a Probiotic (Bacillus clausii) in Acute Kidney Injury in a Rat Model of LPS-Induced Endotoxemia

Yazarlar:

Asli KANDİL

Yayıncı:

Istanbul University

Dergi:

European Journal of Biology 2021 Vol.80 Issue 1, pp.48-53

Belge Tipi:

Makale

Yayın Tarihi:

6/2021

Belge Dili:

İngilizce

Document ID (Org No.):

2000424363

eISSN:

2618-6144

pISSN:

2602-2575

Özet:

 


Objective: This study aims to examine the role of a probiotic bacterium, Bacillus clausii, on oxidative stress in a lipopolysaccharide (LPS)-induced acute kidney injury (AKI) rat model. 

Materials and Methods: The rats were divided into four groups: Control group, LPS group (1.5 mg/kg, LPS), Probiotic+LPS group in which LPS was given after administration of Bacillus clausii as a probiotic for 21 days, and Probiotic group. The kidneys of the rats were removed 24 hours after LPS injection. Total antioxidant status, total oxidant status, oxidative stressindex, malondialdehyde and myeloperoxidase (MPO) values were biochemically determined in the kidneys. Furthermore, the kidney tissue samples were immunohistochemically stained for interleukin-6 (IL-6) and tumor necrosis factor (TNF)-α expression, and leukocyte distribution. 

Results: Endotoxemia caused an increase in oxidative stress (p<0.001), lipid peroxidation (p<0.01), MPO activity (p<0.001), and the expression of IL-6 (p<0.001) and TNF-α (p<0.001). The administration of probiotic ameliorated oxidative stress, lipid peroxidation, and myeloperoxidase activity, and resulted in decreased IL-6 and TNF-α reactions that were elevated with LPS treatment. 

Conclusion: The results suggest that Bacillus clausii as a probiotic bacterium may have an antioxidative property in LPSinduced AKI.

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Makale detayları

The Effects of a Probiotic (Bacillus clausii) in Acute Kidney Injury in a Rat Model of LPS-Induced Endotoxemia

Yazarlar: Asli KANDİL

Yayıncı: Istanbul University

Dergi: European Journal of Biology 2021 Vol.80 Issue 1, pp.48-53

Belge Tipi: Makale

Yayın Tarihi: 6/2021

Belge Dili: İngilizce

Document ID (Org No.): 2000424363

eISSN: 2618-6144

pISSN: 2602-2575

Sayı

Yazarlar:

Asli KANDİL

Yayıncı:

Istanbul University

Dergi:

European Journal of Biology 2021 Vol.80 Issue 1, pp.48-53

Belge Tipi:

Makale

Yayın Tarihi:

6/2021

Belge Dili:

İngilizce

Document ID (Org No.):

2000424363

eISSN:

2618-6144

pISSN:

2602-2575