Effect of Vitamin B Complex on Colon Cancer by Photodynamic Therapy

Asiye Yurttaş; Kamil Çınar

Makale detayları

Effect of Vitamin B Complex on Colon Cancer by Photodynamic Therapy

Yazarlar:

Asiye Yurttaş | Kamil Çınar

Yayıncı:

Süleyman Demirel University

Ana Başlıklar:

Sağlık Kurumları Yönetimi

Dergi:

Süleyman Demirel Üniversitesi Sağlık Bilimleri Dergisi Vol.14 Issue 2, pp.142-152

Belge Tipi:

Makale

Yayın Tarihi:

8/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001296042

pISSN:

2146-247X

Anahtar kelimeler:

Özet:

 Objective: Colon cancer is a chronic fatal disease that severely affects the quality of life of patients and is associated with various complications. Vitamin B complex shows positive anti-inflammatory effects when taken as a food supplement. The aim of this study was to investigate the anti-cancer effect of vitamin B complex with liposomes in colon cancers HCT-116 and HT-29 cell lines using photodynamic therapy. Material-Method: Vitamin B complex, which is the first step of the study, was encapsulated with liposome. Vitamin B complex with liposome was characterized by Zetasizer device. The MTT 3-4,5-dimethyl-thiazolyl-2,5-diphenyltetrazolium bromide assay was used to determine the  survival rates in HCT-116 and HT-29 cell lines. The same cell lines were also analyzed by flow cytometry to show which death pathways were selected by the vitamin B complex with the effect of photodynamic therapy. Results: It was found that the dimensions of the vitamin B complex with liposomes varied between 100 nm and 200 nm. Liposomal vitamin B complex photosensitizer at 7.5 Jcm2 light dose at 0.25 mgmL in the dark experiment, while the percentage of HCT-116 cell viability was 75, this rate decreased to 47 after the light experiment p 0.001. While the percentage of HT-29 cell viability was 70 in the dark test for 0.5 mgmL vitamin B complex solution, this rate decreased to 35 after the light test p0.001. The desired photodynamic efficiency is closer to these results. In the cell death pathway assay, the concentration of liposomal vitamin B complex was applied to HCT-116 and HT-29 cells at 1 mgmL. While 40 apoptosis was observed in the HCT-116 cell line, 60 apoptosis was observed in the HT-29 cell line. Conclusion: Repositioning of marketed drugs has emerged as a new approach to the discovery of new anti-tumor drugs. The development of new uses for marketed drugs has proven to be an efficient and rapid way to develop new anti-tumor drugs. Our data suggest that the in vitro use of vitamin B complex with photodynamic therapy is promising in some types of colorectal cancer.

 Objective: Colon cancer is a chronic fatal disease that severely affects the quality of life of patients and is associated with various complications. Vitamin B complex shows positive anti-inflammatory effects when taken as a food supplement. The aim of this study was to investigate the anti-cancer effect of vitamin B complex with liposomes in colon cancers HCT-116 and HT-29 cell lines using photodynamic therapy. Material-Method: Vitamin B complex, which is the first step of the study, was encapsulated with liposome. Vitamin B complex with liposome was characterized by Zetasizer device. The MTT 3-4,5-dimethyl-thiazolyl-2,5-diphenyltetrazolium bromide assay was used to determine the  survival rates in HCT-116 and HT-29 cell lines. The same cell lines were also analyzed by flow cytometry to show which death pathways were selected by the vitamin B complex with the effect of photodynamic therapy. Results: It was found that the dimensions of the vitamin B complex with liposomes varied between 100 nm and 200 nm. Liposomal vitamin B complex photosensitizer at 7.5 Jcm2 light dose at 0.25 mgmL in the dark experiment, while the percentage of HCT-116 cell viability was 75, this rate decreased to 47 after the light experiment p 0.001. While the percentage of HT-29 cell viability was 70 in the dark test for 0.5 mgmL vitamin B complex solution, this rate decreased to 35 after the light test p0.001. The desired photodynamic efficiency is closer to these results. In the cell death pathway assay, the concentration of liposomal vitamin B complex was applied to HCT-116 and HT-29 cells at 1 mgmL. While 40 apoptosis was observed in the HCT-116 cell line, 60 apoptosis was observed in the HT-29 cell line. Conclusion: Repositioning of marketed drugs has emerged as a new approach to the discovery of new anti-tumor drugs. The development of new uses for marketed drugs has proven to be an efficient and rapid way to develop new anti-tumor drugs. Our data suggest that the in vitro use of vitamin B complex with photodynamic therapy is promising in some types of colorectal cancer.

 Objective: Colon cancer is a chronic fatal disease that severely affects the quality of life of patients and is associated with various complications. Vitamin B complex shows positive anti-inflammatory effects when taken as a food supplement. The aim of this study was to investigate the anti-cancer effect of vitamin B complex with liposomes in colon cancers HCT-116 and HT-29 cell lines using photodynamic therapy. Material-Method: Vitamin B complex, which is the first step of the study, was encapsulated with liposome. Vitamin B complex with liposome was characterized by Zetasizer device. The MTT 3-4,5-dimethyl-thiazolyl-2,5-diphenyltetrazolium bromide assay was used to determine the  survival rates in HCT-116 and HT-29 cell lines. The same cell lines were also analyzed by flow cytometry to show which death pathways were selected by the vitamin B complex with the effect of photodynamic therapy. Results: It was found that the dimensions of the vitamin B complex with liposomes varied between 100 nm and 200 nm. Liposomal vitamin B complex photosensitizer at 7.5 Jcm2 light dose at 0.25 mgmL in the dark experiment, while the percentage of HCT-116 cell viability was 75, this rate decreased to 47 after the light experiment p 0.001. While the percentage of HT-29 cell viability was 70 in the dark test for 0.5 mgmL vitamin B complex solution, this rate decreased to 35 after the light test p0.001. The desired photodynamic efficiency is closer to these results. In the cell death pathway assay, the concentration of liposomal vitamin B complex was applied to HCT-116 and HT-29 cells at 1 mgmL. While 40 apoptosis was observed in the HCT-116 cell line, 60 apoptosis was observed in the HT-29 cell line. Conclusion: Repositioning of marketed drugs has emerged as a new approach to the discovery of new anti-tumor drugs. The development of new uses for marketed drugs has proven to be an efficient and rapid way to develop new anti-tumor drugs. Our data suggest that the in vitro use of vitamin B complex with photodynamic therapy is promising in some types of colorectal cancer.

 Amaç: Kolon kanserleri, hastanın yaşam kalitesini derinden etkileyen ve çeşitli komplikasyonlarla ilişkili kronik ölümcül bir hastalıktır. Vitamin B kompleksi, gıda takviyesi olarak alındığında olumlu anti-inflamatuar etkiler göstermektedir. Bu çalışma, fotodinamik terapiyle lipozomlu vitamin B kompleksinin kolon kanserlerinde HCT-116 ve HT-29 hücre hattı anti-kanser etkisini araştırmayı amaçlamıştır. Materyal- Metot: Çalışmanın birinci basamağını oluşturan Vitamin B kompleksi lipozom ile kapsüle edildi. Lipozomlu vitamin B kompleksi Zetasizer cihazı ile karakterize edildi. Lipozomlu vitamin B kompleksi MTT 3-4,5-dimetil-tiyazolil-2,5-difeniltetrazolyum bromür testi ile HCT-116 ve HT-29 hücre hattında  canlılık oranlarına bakılmıştır. Aynı hücre hatlarında vitamin B kompleksinin fotodinamik terapi etkisiyle hangi ölüm yolaklarını seçtiğini göstermek için de akış sitometrisi kullanılarak analiz edilmiştir. Bulgular: Lipozomlu vitamin B kompleksinin boyutları 100 nm ile 200 nm aralığında değiştiği belirlendi. Lipozomlu vitamin B kompleksi fotosensitizer 7,5 Jcm2 ışık dozunda 0,25 mg mLde karanlık deneyinde HCT-116 hücre canlılık yüzdesi 75 iken ışık deneyi sonrası bu oran 47lere düşmüştür p0.001. 0,5 mgmL vitamin B kompleksi çözeltisi için yapılan karanlık deneyinde HT-29 hücre canlılık yüzdesi 70 iken ışık deneyi sonrası bu oran 35lere düşmüştür p0.001. Arzu edilen fotodinamik etkinlik bu sonuçlara daha yakındır. Hücre ölüm yolakları belirleme deneyinde lipozomlanmış vitamin B kompleksinin konsantrasyonu 1mgmL olarak HCT-116 ve HT-29 hücrelerine uygulanmıştır. HCT-116 hücre hattında 40 oranında apoptoz görülürken HT-29 hücre hattında 60 oranında apoptoz görüldü. Sonuç: Pazarlanan ilaçları yeniden konumlandırmak, yeni antitümör ilaçları keşfetmek için yeni bir yaklaşım olarak ortaya çıkmıştır. Pazarlanan ilaçlar için yeni kullanım alanlarının geliştirilmesi, yeni anti-tümör ilaçları geliştirmenin etkili ve hızlı yolu olduğu kanıtlanmıştır. Verilerimiz in-vitro olarak bazı kolon kanser türlerinde vitamin B kompleksinin fotodinamik terapiyle kullanımının ümit vaat ettiğini savunmaktadır.

Makale detayları

Effect of Vitamin B Complex on Colon Cancer by Photodynamic Therapy

Yazarlar:

Asiye Yurttaş | Kamil Çınar

Yayıncı:

Süleyman Demirel University

Ana Başlıklar:

Sağlık Kurumları Yönetimi

Dergi:

Süleyman Demirel Üniversitesi Sağlık Bilimleri Dergisi Vol.14 Issue 2, pp.142-152

Belge Tipi:

Makale

Yayın Tarihi:

8/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001296042

pISSN:

2146-247X

Photodynamic therapy

colon cancer

drug repositioning

vitamin B complex

laserFotodinamik terapi

kolon kanseri

ilaç yeniden konumlandırma

vitamin B kompleksi

lazer

Sayı

Makale detayları

Yazarlar: Asiye Yurttaş | Kamil Çınar

Yayıncı: Süleyman Demirel University

Ana Başlıklar: Sağlık Kurumları Yönetimi

Dergi: Süleyman Demirel Üniversitesi Sağlık Bilimleri Dergisi Vol.14 Issue 2, pp.142-152

Belge Tipi: Makale

Yayın Tarihi: 8/2023

Belge Dili: Türkçe

Document ID (Org No.): 2001296042

pISSN: 2146-247X

Sayı

Yazarlar:

Asiye Yurttaş | Kamil Çınar

Yayıncı:

Süleyman Demirel University

Ana Başlıklar:

Sağlık Kurumları Yönetimi

Dergi:

Süleyman Demirel Üniversitesi Sağlık Bilimleri Dergisi Vol.14 Issue 2, pp.142-152

Belge Tipi:

Makale

Yayın Tarihi:

8/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001296042

pISSN:

2146-247X