Quantitation of Neuronal Loss Induced by Status Epilepticus and the Effects of Nitric Oxide

Güldal GÜLEÇ

Makale detayları

Quantitation of Neuronal Loss Induced by Status Epilepticus and the Effects of Nitric Oxide

Yazarlar:

Güldal GÜLEÇ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2000 Vol.30 Issue 3, pp.229-234

Belge Tipi:

Makale

Yayın Tarihi:

2/2000

Belge Dili:

Türkçe

Document ID (Org No.):

2000757616

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Anahtar kelimeler:

Özet:

 

Neuronal loss in the hippocampal  CA1 and CA3 fields due to lithium-pilocarpine-  induced status epilepticus (SE)  and the effects of a nitric oxide (NO)  substrate, L-Arginine and a NO synthase  inhibitor, Nw -nitro-L-Arginine methyl ester  (L-NAME) on SE-induced brain damage were  determined using the neuron counting  procedure in rats. Rats were implanted with  four chronic, epidural screw electrodes in  order to obtain electrocortical recordings. SE  was determined from the electrocortical  recordings. After decapitation, the brains  were removed, and the hippocampal sections  were obtained and stained with hematoxylin  and eosin. An analysis of variance (ANOVA)  and Student's t- test were used for statistical  purposes. The normal pyramidal neuron  numbers of rats which had exhibited SE were  significantly lower than those of rats that had  not displayed SE. Both L-Arginine and L-NAME  increased the neuronal loss induced by  SE. We concluded that lithium-pilocarpine-induced  SE leads to neuronal loss in the  hippocampal CA1 and CA3 fields. L-Arginine  and L-NAME, which were expected to show  opposite actions, acted in a similar manner at  the given doses.

 

Neuronal loss in the hippocampal  CA1 and CA3 fields due to lithium-pilocarpine-  induced status epilepticus (SE)  and the effects of a nitric oxide (NO)  substrate, L-Arginine and a NO synthase  inhibitor, Nw -nitro-L-Arginine methyl ester  (L-NAME) on SE-induced brain damage were  determined using the neuron counting  procedure in rats. Rats were implanted with  four chronic, epidural screw electrodes in  order to obtain electrocortical recordings. SE  was determined from the electrocortical  recordings. After decapitation, the brains  were removed, and the hippocampal sections  were obtained and stained with hematoxylin  and eosin. An analysis of variance (ANOVA)  and Student's t- test were used for statistical  purposes. The normal pyramidal neuron  numbers of rats which had exhibited SE were  significantly lower than those of rats that had  not displayed SE. Both L-Arginine and L-NAME  increased the neuronal loss induced by  SE. We concluded that lithium-pilocarpine-induced  SE leads to neuronal loss in the  hippocampal CA1 and CA3 fields. L-Arginine  and L-NAME, which were expected to show  opposite actions, acted in a similar manner at  the given doses.

Makale detayları

Quantitation of Neuronal Loss Induced by Status Epilepticus and the Effects of Nitric Oxide

Yazarlar:

Güldal GÜLEÇ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2000 Vol.30 Issue 3, pp.229-234

Belge Tipi:

Makale

Yayın Tarihi:

2/2000

Belge Dili:

Türkçe

Document ID (Org No.):

2000757616

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Lithium-pilocarpine

Status epilepticus

L-Arginine

L-NAME

Neuron loss.

Sayı

Makale detayları

Quantitation of Neuronal Loss Induced by Status Epilepticus and the Effects of Nitric Oxide

Yazarlar: Güldal GÜLEÇ

Yayıncı: TUBITAK

Dergi: Turkish Journal of Medical Sciences 2000 Vol.30 Issue 3, pp.229-234

Belge Tipi: Makale

Yayın Tarihi: 2/2000

Belge Dili: Türkçe

Document ID (Org No.): 2000757616

Sıklık: İki Aylık

eISSN: 1303-6165

pISSN: 1300-0144

Lithium-pilocarpine

Status epilepticus

L-Arginine

L-NAME

Neuron loss.

Sayı

Yazarlar:

Güldal GÜLEÇ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2000 Vol.30 Issue 3, pp.229-234

Belge Tipi:

Makale

Yayın Tarihi:

2/2000

Belge Dili:

Türkçe

Document ID (Org No.):

2000757616

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144