IFN-γ  stimulated dental follicle mesenchymal stem cells regulate activated lymphocyte response in rheumatoid arthritis patients in vitro

Noushin ZIBANDEH; Deniz GENÇ; Güzide Nevsun İNANÇ; Rafi Haner DİRESKENELİ; Tunç AKKOÇ

Makale detayları

IFN-γ stimulated dental follicle mesenchymal stem cells regulate activated lymphocyte response in rheumatoid arthritis patients in vitro

Yazarlar:

Noushin ZIBANDEH | Deniz GENÇ | Güzide Nevsun İNANÇ | Rafi Haner DİRESKENELİ | Tunç AKKOÇ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2019 Vol.49 Issue 6, pp.1779-1788

Belge Tipi:

Makale

Yayın Tarihi:

11/2019

Belge Dili:

İngilizce

Document ID (Org No.):

2000749810

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Özet:

 

Background/aim: Multipotent  mesenchymal  stem  cells  (MSCs)  have  been  investigated  in  autoimmune  diseases  such  as  rheumatoid  arthritis (RA) due to their immunomodulatory and regenerative properties. In this study, their immunosuppressive effects on peripheral blood mononuclear cells (PBMC) of RA patients were studied.Materials and methods: Dental follicle stem cells (DFSCs) were isolated from follicle tissue in the orofacial region. Characterization and  multipotency  analyses  were  performed.  Lymphocytes  were  isolated  from  peripheral  venous  blood  of  RA  patients  (n  =  5)  and  healthy  individuals  (n  =  5).  DFSCs  were  preincubated  with  IFN-γ  for  48  h.  PBMCs  of  RA  patients  and  healthy  individuals  were  separately cultured with or without DFSCs for 72 h. After culture period, lymphocyte proliferation and viability, the frequency of CD4+CD25+FoxP3+ T regulatory cells, IL-10 and TNF-α levels in the culture supernatants were measured via flow cytometry.Results: Our results demonstrated that DFSCs suppressed proliferation of T lymphocytes by increasing the number of FoxP3 expressing CD4+CD25+ T regulatory cells and suppressed lymphocyte apoptosis in RA patients. Also, DFSCs reduced TNF-α cytokine secretion and upregulated IL-10 secreting cells.Conclusions: Such cells could potentially be a source for future immunomodulatory treatments of RA patients.

Makale detayları

IFN-γ stimulated dental follicle mesenchymal stem cells regulate activated lymphocyte response in rheumatoid arthritis patients in vitro

Yazarlar: Noushin ZIBANDEH | Deniz GENÇ | Güzide Nevsun İNANÇ | Rafi Haner DİRESKENELİ | Tunç AKKOÇ

Yayıncı: TUBITAK

Dergi: Turkish Journal of Medical Sciences 2019 Vol.49 Issue 6, pp.1779-1788

Belge Tipi: Makale

Yayın Tarihi: 11/2019

Belge Dili: İngilizce

Document ID (Org No.): 2000749810

Sıklık: İki Aylık

eISSN: 1303-6165

pISSN: 1300-0144

Sayı

Yazarlar:

Noushin ZIBANDEH | Deniz GENÇ | Güzide Nevsun İNANÇ | Rafi Haner DİRESKENELİ | Tunç AKKOÇ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2019 Vol.49 Issue 6, pp.1779-1788

Belge Tipi:

Makale

Yayın Tarihi:

11/2019

Belge Dili:

İngilizce

Document ID (Org No.):

2000749810

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144