Evaluating the effectiveness of gabapentin and ginkgo biloba in the neuroinflammatory process within a co-culture model of GBM and neurons

Çiğdem Sevim; Ali Taghizadehghalehjoughi

Makale detayları

Evaluating the effectiveness of gabapentin and ginkgo biloba in the neuroinflammatory process within a co-culture model of GBM and neurons

Yazarlar:

Çiğdem Sevim | Ali Taghizadehghalehjoughi

Yayıncı:

Hacettepe University

Ana Başlıklar:

Gene Expression

Dergi:

Hacettepe Journal of Biology and Chemistry Vol.53 Issue 2, pp.39-48

Belge Tipi:

Makale

Yayın Tarihi:

1/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001332717

pISSN:

2687-475X

Anahtar kelimeler:

Özet:

 This study aimed to investigate the anti-inflammatory effects of Ginkgo Biloba extract and Gabapentin on a neuron-Glioblastoma cell line. Utilizing advanced co-culture techniques, we simulated a tumor environment that closely mimics in vivo conditions and performed a detailed genetic analysis of cytokine expression. Testing a range of doses from 20 to 80 µgml, we found that the combination of Gabapentin 80 µgml and Ginkgo Biloba extract 80 µgml did not alter levels of proinflammatory cytokines such as IL1β, IL6, IL8, and TNF-α. However, it significantly increased the levels of the anti-inflammatory cytokine IL10 compared to other groups. These findings suggest that the co-administration of Gabapentin and Ginkgo Biloba can modulate the inflammatory response, maintaining it at levels similar to the control group. Determining the precise effective dosage range and understanding the mechanisms to halt neuroinflammation will be crucial for advancing therapeutic options for glioblastoma multiforme. This research provides a promising foundation for developing new treatment strategies aimed at reducing inflammation in glioblastoma patients.

 This study aimed to investigate the anti-inflammatory effects of Ginkgo Biloba extract and Gabapentin on a neuron-Glioblastoma cell line. Utilizing advanced co-culture techniques, we simulated a tumor environment that closely mimics in vivo conditions and performed a detailed genetic analysis of cytokine expression. Testing a range of doses from 20 to 80 µgml, we found that the combination of Gabapentin 80 µgml and Ginkgo Biloba extract 80 µgml did not alter levels of proinflammatory cytokines such as IL1β, IL6, IL8, and TNF-α. However, it significantly increased the levels of the anti-inflammatory cytokine IL10 compared to other groups. These findings suggest that the co-administration of Gabapentin and Ginkgo Biloba can modulate the inflammatory response, maintaining it at levels similar to the control group. Determining the precise effective dosage range and understanding the mechanisms to halt neuroinflammation will be crucial for advancing therapeutic options for glioblastoma multiforme. This research provides a promising foundation for developing new treatment strategies aimed at reducing inflammation in glioblastoma patients.

 This study aimed to investigate the anti-inflammatory effects of Ginkgo Biloba extract and Gabapentin on a neuron-Glioblastoma cell line. Utilizing advanced co-culture techniques, we simulated a tumor environment that closely mimics in vivo conditions and performed a detailed genetic analysis of cytokine expression. Testing a range of doses from 20 to 80 µgml, we found that the combination of Gabapentin 80 µgml and Ginkgo Biloba extract 80 µgml did not alter levels of proinflammatory cytokines such as IL1β, IL6, IL8, and TNF-α. However, it significantly increased the levels of the anti-inflammatory cytokine IL10 compared to other groups. These findings suggest that the co-administration of Gabapentin and Ginkgo Biloba can modulate the inflammatory response, maintaining it at levels similar to the control group. Determining the precise effective dosage range and understanding the mechanisms to halt neuroinflammation will be crucial for advancing therapeutic options for glioblastoma multiforme. This research provides a promising foundation for developing new treatment strategies aimed at reducing inflammation in glioblastoma patients.

 Bu çalışma, Ginkgo Biloba ekstresi ve Gabapentinin nöron-Glioblastoma hücre hattı üzerindeki anti-inflamatuar etkilerini araştırmayı amaçladı. Gelişmiş ko-kültür teknikleri kullanarak in vivo koşulları yakından taklit eden bir tümör ortamı simüle ettik ve sitokin ekspresyonunun detaylı genetik analizini gerçekleştirdik. 20 ila 80 µgml aralığında farklı dozları test ettik ve Gabapentin 80 µgml ile Ginkgo Biloba ekstresinin 80 µgml kombinasyonunun, IL1β, IL6, IL8 ve TNF-α gibi proinflamatuar sitokin seviyelerini değiştirmediğini, ancak diğer gruplara kıyasla anti-inflamatuar sitokin IL10 seviyelerini önemli ölçüde artırdığını bulduk. Bu bulgular, Gabapentin ve Ginkgo Bilobanın birlikte uygulanmasının inflamatuar yanıtı düzenleyebileceğini ve bunu kontrol grubuna benzer seviyelerde tutabileceğini göstermektedir. Kesin etkili doz aralığının belirlenmesi ve nöroinflamasyonun durdurulma mekanizmalarının anlaşılması, glioblastoma multiforme için terapötik seçeneklerin ilerletilmesi açısından kritik olacaktır. Bu araştırma, glioblastoma hastalarında inflamasyonu azaltmayı hedefleyen yeni tedavi stratejilerinin geliştirilmesi için umut verici bir temel sağlamaktadır.

Makale detayları

Evaluating the effectiveness of gabapentin and ginkgo biloba in the neuroinflammatory process within a co-culture model of GBM and neurons

Yazarlar:

Çiğdem Sevim | Ali Taghizadehghalehjoughi

Yayıncı:

Hacettepe University

Ana Başlıklar:

Gene Expression

Dergi:

Hacettepe Journal of Biology and Chemistry Vol.53 Issue 2, pp.39-48

Belge Tipi:

Makale

Yayın Tarihi:

1/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001332717

pISSN:

2687-475X

Cell culture

Proinflammatory cytokines

Glioblastoma multiforme

Interleukinhücre kültürü

proinflamatuar sitokin

glioblastoma multiforme

interlökin

Sayı

Makale detayları

Yazarlar: Çiğdem Sevim | Ali Taghizadehghalehjoughi

Yayıncı: Hacettepe University

Ana Başlıklar: Gene Expression

Dergi: Hacettepe Journal of Biology and Chemistry Vol.53 Issue 2, pp.39-48

Belge Tipi: Makale

Yayın Tarihi: 1/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001332717

pISSN: 2687-475X

Sayı

Yazarlar:

Çiğdem Sevim | Ali Taghizadehghalehjoughi

Yayıncı:

Hacettepe University

Ana Başlıklar:

Gene Expression

Dergi:

Hacettepe Journal of Biology and Chemistry Vol.53 Issue 2, pp.39-48

Belge Tipi:

Makale

Yayın Tarihi:

1/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001332717

pISSN:

2687-475X