Cytotoxic and Electrophysiological Effects of Imipramine on Eag1 Potassium Channels in The Mcf-7 Cell Line

Barış Uçar; Ali Aşkın; Fatma Söğüt; Ülkü Çömelekoğlu; Hatice Oruç Demirbağ; Şakir Necat Yılmaz

Makale detayları

Cytotoxic and Electrophysiological Effects of Imipramine on Eag1 Potassium Channels in The Mcf-7 Cell Line

Yazarlar:

Barış Uçar | Ali Aşkın | Fatma Söğüt | Ülkü Çömelekoğlu | Hatice Oruç Demirbağ | Şakir Necat Yılmaz

Yayıncı:

Osmaniye Korkut Ata Üniversitesi

Ana Başlıklar:

Yapısal Biyoloji

Dergi:

Osmaniye Korkut Ata Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.7 Issue 1, pp.263-275

Belge Tipi:

Makale

Yayın Tarihi:

1/2024

Belge Dili:

Türkçe

Document ID (Org No.):

2001310150

pISSN:

2687-3729

Anahtar kelimeler:

Özet:

 It is noteworthy that some ion channels are overexpressed in cancer cells. One of these ion channels is the Ether à-go-go 1 KCNH1, Kv10.1 Eag1 K channel. In this study, cytotoxic and electrophysiological effects of different concentrations of tricyclic antidepressant, Imipramine, on Eag1 K channels were investigated in MCF-7 cell line breast cancer cell. Its cytotoxic effect was investigated by MTT analysis method, and its electrophysiological effect was researched by patch-clamping technique. By MTT analysis, it was determined that 20 µM and higher concentrations of Imipramine on MCF-7 cells, had an approximately 85 suppressive effect, compared to the control group. On the other hand, in patch-clamping experiments, 2μM, 5μM and 20μM effects on Eag1 K channels were investigated. It has been shown that low-dose, 2μM Imipramine, inhibits Eag1 K channel currents by approximately 75 and its high-dose, 20μM, inhibits the channel currents by approximately 82, compared to the control group.

 It is noteworthy that some ion channels are overexpressed in cancer cells. One of these ion channels is the Ether à-go-go 1 KCNH1, Kv10.1 Eag1 K channel. In this study, cytotoxic and electrophysiological effects of different concentrations of tricyclic antidepressant, Imipramine, on Eag1 K channels were investigated in MCF-7 cell line breast cancer cell. Its cytotoxic effect was investigated by MTT analysis method, and its electrophysiological effect was researched by patch-clamping technique. By MTT analysis, it was determined that 20 µM and higher concentrations of Imipramine on MCF-7 cells, had an approximately 85 suppressive effect, compared to the control group. On the other hand, in patch-clamping experiments, 2μM, 5μM and 20μM effects on Eag1 K channels were investigated. It has been shown that low-dose, 2μM Imipramine, inhibits Eag1 K channel currents by approximately 75 and its high-dose, 20μM, inhibits the channel currents by approximately 82, compared to the control group.

 It is noteworthy that some ion channels are overexpressed in cancer cells. One of these ion channels is the Ether à-go-go 1 KCNH1, Kv10.1 Eag1 K channel. In this study, cytotoxic and electrophysiological effects of different concentrations of tricyclic antidepressant, Imipramine, on Eag1 K channels were investigated in MCF-7 cell line breast cancer cell. Its cytotoxic effect was investigated by MTT analysis method, and its electrophysiological effect was researched by patch-clamping technique. By MTT analysis, it was determined that 20 µM and higher concentrations of Imipramine on MCF-7 cells, had an approximately 85 suppressive effect, compared to the control group. On the other hand, in patch-clamping experiments, 2μM, 5μM and 20μM effects on Eag1 K channels were investigated. It has been shown that low-dose, 2μM Imipramine, inhibits Eag1 K channel currents by approximately 75 and its high-dose, 20μM, inhibits the channel currents by approximately 82, compared to the control group.

 Kanser hücrelerinde bazı iyon kanallarının aşırı eksprese edilmesi dikkat çekmektedir. Bu iyon kanallarından biri de Ether à-go-go 1 KCNH1, Kv10.1 Eag1 K kanalıdır. Bu çalışmada, MCF-7 hücre hattında meme kanseri hücresi trisiklik antidepresan olan imipraminin farklı konsantrasyonları kullanılarak, Eag1 K kanalları üzerine sitotoksik ve elektrofizyolojik etkileri incelenmiştir. Sitotoksik etkisi MTT analiz yöntemiyle, Elektrofizyolojik etkisi ise yama-kenetleme tekniği ile araştırılmıştır. MTT analiz yöntemiyle imipraminin MCF-7 hücreleri üzerinde kontrol grubuna göre 20μM ve üzeri konsantrasyonlarının yaklaşık 85 oranında baskılayıcı etkiye sahip olduğu belirlenmiştir. Yama-kenetleme deneylerinde MCF-7 hücrelerine 2μM, 5μM ve 20μM imipramin uygulanarak Eag1 K kanalları üzerine etkisi incelenmiştir. Düşük doz olan 2μM imipraminin kontrol grubuna göre Eag1 K kanal akımları yaklaşık 75 ve yüksek doz olan 20μM imipraminin Eag1 K kanal akımlarını yaklaşık 82 gibi yüksek oranda inhibe ettiği gösterilmiştir.

Makale detayları

Cytotoxic and Electrophysiological Effects of Imipramine on Eag1 Potassium Channels in The Mcf-7 Cell Line

Yazarlar:

Barış Uçar | Ali Aşkın | Fatma Söğüt | Ülkü Çömelekoğlu | Hatice Oruç Demirbağ | Şakir Necat Yılmaz

Yayıncı:

Osmaniye Korkut Ata Üniversitesi

Ana Başlıklar:

Yapısal Biyoloji

Dergi:

Osmaniye Korkut Ata Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.7 Issue 1, pp.263-275

Belge Tipi:

Makale

Yayın Tarihi:

1/2024

Belge Dili:

Türkçe

Document ID (Org No.):

2001310150

pISSN:

2687-3729

Imipramine

Eag1 Potassium Channel

Breast Cancer

MCF-7 Cell Line

Electrophysiology

Cytotoxicityİmipramin

Eag1 Potasyum Kanalı

Meme Kanseri

MCF-7 Hücre Hattı

Elektrofizyoloji

Sitotoksisite

Sayı

Makale detayları

Yazarlar: Barış Uçar | Ali Aşkın | Fatma Söğüt | Ülkü Çömelekoğlu | Hatice Oruç Demirbağ | Şakir Necat Yılmaz

Yayıncı: Osmaniye Korkut Ata Üniversitesi

Ana Başlıklar: Yapısal Biyoloji

Dergi: Osmaniye Korkut Ata Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.7 Issue 1, pp.263-275

Belge Tipi: Makale

Yayın Tarihi: 1/2024

Belge Dili: Türkçe

Document ID (Org No.): 2001310150

pISSN: 2687-3729

Sayı

Yazarlar:

Barış Uçar | Ali Aşkın | Fatma Söğüt | Ülkü Çömelekoğlu | Hatice Oruç Demirbağ | Şakir Necat Yılmaz

Yayıncı:

Osmaniye Korkut Ata Üniversitesi

Ana Başlıklar:

Yapısal Biyoloji

Dergi:

Osmaniye Korkut Ata Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.7 Issue 1, pp.263-275

Belge Tipi:

Makale

Yayın Tarihi:

1/2024

Belge Dili:

Türkçe

Document ID (Org No.):

2001310150

pISSN:

2687-3729